hPSC衍生神经细胞研究

神经诱导后，人类多能干细胞 (hPSC) 衍生的神经祖细胞 (NPC) 可进一步分化为中枢神经系统的主要神经细胞类型：神经元、星形胶质细胞和少突胶质细胞。 查看更多 These specialized neural cell types hold great promise for studying human neurological development and disease. After using STEMdiff™ neural induction protocols to generate NPCs, you can use cell type-specific STEMdiff™ differentiation and maturation kits to differentiate forebrain-type neurons, midbrain neurons, or astrocytes.

A two-step differentiation and maturation approach allows for increased overall efficiency and experimental flexibility. First, STEMdiff™ differentiation kits for forebrain-type neurons, midbrain-type neurons or astrocytes generate lineage-restricted precursors from NPCs. Researchers can then mature these precursors to functional forebrain-type neurons, midbrain- neurons or astrocytes, using the corresponding STEMdiff™ maturation kits. Many of our maturation kits have a basal medium based on BrainPhys™ Neuronal Medium, a serum-free physiological medium that is based on the formulation from the laboratory of Fred H. Gage (C Bardy et al. Proc Natl Acad Sci USA, 2015) and supports neuronal activity. hPSC-derived neuronal cultures show activity-dependent maturation, expressing more synaptically-associated proteins (e.g. J Ruden et al. Front Mol Neurosci, 2021) and a wider range of adult protein isoforms (e.g. L Miguel et al. Stem Cell Res, 2019) when cultured in BrainPhys™-based media—potentially uncovering crucial information about their complex phenotypes.

Downstream of neural crest induction, researchers can further differentiate hPSC-derived neural crest cells (NCCs) to cell types of the peripheral nervous system. NCCs generated using the STEMdiff™ Neural Crest Differentiation Kit differentiate to sensory neurons using a two-step approach with STEMdiff™ Sensory Neuron Differentiation and Maturation Kits.

For a flexible approach to generating many different neural cell subtypes according to published protocols, you may combine neural supplements, such as NeuroCult™ SM1 Neuronal Supplement (based on the published B-27 formulation) and N2 Supplement A or B , with small molecules or cytokines and a suitable basal medium, such as BrainPhys™ Neuronal Medium . Compared to traditional medium formulations, BrainPhys™ Neuronal Medium is more representative of the CNS extracellular environment, which results in hPSC-derived neuronal cultures with increased proportions of synaptically active neurons.

如果您的实验更适合 3D 模型，您可以使用 STEMdiff™ 神经类器官试剂盒， 直接从 hPSC 中区分出包含排列成 3D 组织结构的各种神经细胞类型的类器官。神经类器官具有可实现发育自组织或特定神经系统亚区域模式化的选项，是研究人类发育过程和复杂细胞相互作用的有力工具。