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NeuroFluor™ NeuO

Membrane-permeable fluorescent probe for the detection of live neurons
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产品号 #01801_C

Membrane-permeable fluorescent probe for the detection of live neurons

产品优势


  • Enables selective labeling of mouse, rat, or human neurons without fixation

  • Can be used to confirm neuronal differentiation of human pluripotent stem cell-derived neural progenitor cells

  • Can be used to label neurons in the presence of other brain cells

  • Non-toxic and non-permanent

  • Simple and rapid labeling protocol

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NeuroFluor™ NeuO
概述
实验数据
产品说明书及文档
应用领域
相关材料与文献

概述

NeuroFluor™ NeuO is a membrane-permeable fluorescent probe that selectively labels primary and pluripotent stem cell-derived neurons in the presence of other brain cells (J C Er et al. Angew Chem Int Ed, 2015). Cells labeled with NeuroFluor™ NeuO can be visualized using fluorescent imaging. Labeling with this probe is non-permanent; it can be washed off, providing unlabeled, viable cells for downstream applications. For additional information, see References.

Contains
• 100 µM NeuO (CAS Number: 1616355-50-0, 1668597-38-3) <br> <br>• DMSO
 
Cell Type
Neural Cells, PSC-Derived, Neurons
 
Species
Human, Mouse, Rat
 
Application
Characterization, Phenotyping
 
Brand
NeuroFluor
 
Area of Interest
Disease Modeling, Neuroscience, Stem Cell Biology
 

Data Figures

Figure 1. NeuroFluor™ NeuO Selectively Labels Primary E18 Rat Neurons

(A) Primary rat E18 cortical neurons were labeled with 0.25μM NeuroFluor™ NeuO (green) and incubated for 1 hour. Image was taken after 2 hours of incubation. (B) The same culture was later fixed and stained for β-tubulin III (red). The image shows that NeuroFluor™ NeuO specifically labels β-tubulin III-positive neurons. Nuclei are counterstained with DAPI. Images were taken at 20X magnification.

Figure 2. NeuroFluor™ NeuO Selectively Labels hPSC-Derived Neurons

(A) The neuronal precursors generated from hPSC-derived (XCL-1) neural progenitor cells were cultured in STEMdiff™ Neuron Maturation Medium. After 18 days of culture, hPSC-derived neurons were labeled with NeuroFluor™ NeuO (green). Nuclei are counterstained with DAPI. (B) The same culture was later fixed and stained with β-tubulin III (red). The image shows that NeuroFluor™ NeuO specifically labels β-tubulin III-positive neurons. Images were taken at 20X magnification.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
NeuroFluor™ NeuO
Catalog #
01801
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
NeuroFluor™ NeuO
Catalog #
01801
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

Primary Neuronal Culture: Standardized Media and Reagents
Brochure
Primary Neuronal Culture: Standardized Media and Reagents
NeuroFluor™ NeuO Selectively Label Live Neurons
Brochure
NeuroFluor™ NeuO Selectively Label Live Neurons

Publications (1)

Neuo: A fluorescent chemical probe for live neuron labeling Er JC et al. Angewandte Chemie - International Edition 2015 FEB

Abstract

To address existing limitations in live neuron imaging, we have developed NeuO, a novel cell-permeable fluorescent probe with an unprecedented ability to label and image live neurons selectively over other cells in the brain. NeuO enables robust live neuron imaging and isolation in vivo and in vitro across species; its versatility and ease of use sets the basis for its development in a myriad of neuronal targeting applications.
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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