产品号 #78136_C
Epidermal growth factor, animal component-free
概述
Epidermal growth factor (EGF) is characterized by high-affinity binding to various EGF receptors (EGFRs) and the production of mitogenic responses (Carpenter & Cohen). EGF promotes EGFR dimerization, resulting in activation of downstream pathways including PI3K, ERK1/2, JAK/STAT, β-catenin, and calcium signaling. EGF is secreted by the gut-associated salivary and Brunner’s glands, is found in a variety of body fluids, and stimulates cell proliferation and differentiation in rodent and neonatal human intestine (Wright et al.). Central nervous system stem cells also proliferate in response to the EGF stimulus (Reynolds & Weiss). This product is animal component-free.
Subtype
Cytokines, Growth Factors
Cell Type
Brain Tumor Stem Cells, Endoderm, PSC-Derived, Hematopoietic Stem and Progenitor Cells, Mesenchymal Stem and Progenitor Cells, Mesoderm, PSC-Derived, Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons, Pluripotent Stem Cells, Prostate Cells
Species
Human
Area of Interest
Epithelial Cell Biology, Neuroscience, Stem Cell Biology
Formulation Category
Animal Component-Free
Purity
≥ 95%
Data Figures
(A) The biological activity of Human Recombinant EGF, ACF was tested by its ability to promote the proliferation of BALB/c 3T3 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.0459 ng/mL. (B) 1 μg of Human Recombinant EGF, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Human Recombinant EGF, ACF Catalog #
78136.1, 78136.2, 78136 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant EGF, ACF Catalog #
78136.1, 78136.2, 78136 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Brain Tumor Stem Cell Research
Workflow Stages for Human Pluripotent Stem Cell Research
Workflow Stages for Hematopoietic Stem and Progenitor Cell Research
Workflow Stages for hPSC-Derived Neural Cell Research
Workflow Stages for hPSC-Derived Mesoderm Cell Research
Workflow Stages for Mammary Epithelial Cell Research
Workflow Stages for Neural Stem and Progenitor Cell Research
Resources and Publications
Educational Materials (2)
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STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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