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Human iPSC-Derived Forebrain Neuron Precursor Cells

Frozen human forebrain neuron precursor cells differentiated from human induced pluripotent stem cell line, SCTi003-A
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产品优势


  • Start your experiments faster with a mixed population of excitatory and inhibitory forebrain neuron precursor cells

  • Obtain high-quality neurons, derived from the highly characterized iPSC control line, SCTi003-A

  • Achieve physiologically relevant results with validated neuronal activity

  • Maintain neurons long-term with STEMdiff™ Forebrain Neuron Maturation Kit

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    概述
    实验数据
    产品说明书及文档
    应用领域
    相关材料与文献
    Achieve better data faster by starting your neural workflow with an easy-to-use, high-quality, highly pure population of forebrain-type neuron precursor cells. This mixed population of excitatory and inhibitory neurons provides a ready-to-use model for physiologically relevant results, with functionally active iPSC-derived mature neurons obtained just two weeks after thawing.

    To ensure optimal quality in your neural research, Human iPSC-Derived Forebrain Neuron Precursor Cells are manufactured from the human induced pluripotent stem cell (iPSC) control line, SCTi003-A, using STEMdiff™ SMADi Neural Induction Kit and STEMdiff™ Forebrain Neuron Differentiation Kit. These neuronal precursors can be matured and maintained long-term in culture with STEMdiff™ Forebrain Neuron Maturation Kit, and are versatile tools for modeling human neurological development and disease, drug screening, toxicity testing, and cell therapy validation.

    For advanced in vitro models of cell-cell interactions, these neurons can also be co-cultured with cells generated with select STEMdiff™ hPSC differentiation kits, such as:
    • STEMdiff™ Astrocyte Differentiation Kit
    • STEMdiff™ Microglia Differentiation Kit

    Additional details can be found on the Frequently Asked Questions on iPSCs page.

    Data Figures

    Phase contrast image of Human iPSC-Derived Forebrain Neuron Precursor Cells exhibiting high-quality morphology at various time points.

    Figure 1. Human iPSC-Derived Forebrain Neuron Precursor Cells Exhibit High-Quality Morphology

    Cryopreserved Human iPSC-Derived Forebrain Neuron Precursor Cells were generated from SCTi003-A iPSCs were thawed and plated onto PLO/Laminin-coated plates at 80,000 cells/cm2 in STEMdiff™ Forebrain Neuron Maturation Kit. Neurons were incubated at 37°C and subsequently analyzed by brightfield microscopy at 10x magnification. Forebrain neurons display the expected neuronal morphology with minimal clumping over long-term culture. iPSC = induced pluripotent stem cell

    Immunocytochemistry image of Day 19 neurons matured from Human iPSC-Derived Forebrain Neuron Precursor Cells exhibiting a highly pure βIII-TUB-positive neuronal population.

    Figure 2. Neurons Matured from Human iPSC-Derived Forebrain Neuron Precursor Cells Consist of a Highly Pure Neuronal Population

    Human iPSC-Derived Neuron Precursor Cells generated from SCTi003-A iPSCs were thawed, established in culture, and fixed for immunocytochemistry. (A) Day 14 neurons express high levels of neuronal marker βIII-TUB (red) with low expression of S100B (green). In addition, they display the typical neuronal morphology with healthy neurites and minimal cell clumping. (B) The percentage expression of these markers were quantified. Neuronal marker βIII-TUB was found to be expressed in 95% of neurons, while glial marker S100B was expressed in less than 4% of neurons. Error bars represent standard deviation (n = 2 biological replicates). iPSC = induced pluripotent stem cell

    Immunocytochemistry image of Day 24 neurons matured from Human iPSC-Derived Forebrain Neuron Precursor Cells exhibiting MAP2, GABA, FOXG1 and Synapsin markers.

    Figure 3. Neurons Matured from Human iPSC-Derived Forebrain Neuron Precursor Cells Express Characteristic Neuronal Markers

    Human iPSC-Derived Neuron Precursor Cells generated from SCTi003-A iPSCs were thawed, established in culture, and fixed for immunocytochemistry. Day 24 neurons express neuronal marker MAP2, (A) GABA, (B) forebrain neuron marker FOXG1, and (C) pre-synaptic marker Synapsin. In addition, they display the typical neuronal morphology with healthy neurites and minimal cell clumping. iPSC = induced pluripotent stem cell

    Bulk RNA-seq heatmap showing that Human iPSC-Derived Forebrain Neuron Precursor Cells express expected markers at various time points.

    Figure 4. Human iPSC-Derived Forebrain Neuron Precursor Cells Express Expected Neuronal Markers

    Human iPSC-Derived Forebrain Neuron Precursor Cells were generated from iPS cell line SCTi003-A. The neuron precursors were then matured with STEMdiff™ Forebrain Neuron Maturation Kit. RNA from forebrain neurons was harvested at various time points during maturation (Day 0, Day 14, and Day 21 in maturation media). This RNA from forebrain neurons along with RNA from the parent hPSC control were subsequently sequenced using bulk RNA-seq. The heat map displays expression levels for select genes associated with hPSCs, NPCs or forebrain neuron precursors, and forebrain neurons. iPS = induced pluripotent stem; hPSC = human pluripotent stem cell; NPC = neural progenitor cell

    Electrical activity of Human iPSC-Derived Forebrain Neuron Precursor Cells were measured using the Maestro Pro™ MEA system from Axio Biosystems

    Figure 5. Human iPSC-Derived Forebrain Neuron Precursor Cells Increase Neuronal Activity Over 42 Days in Culture

    Human iPSC-Derived Forebrain Neuron Precursor Cells were generated from the hiPSC line SCTi003-A (Catalog #200-0511). The neuron precursors were then matured on a 48-well MEA plate (Axion Biosystems) with STEMdiff™ Forebrain Neuron Maturation Kit (Catalog #08605). Electrical activity from 16 electrodes was measured over time using the Maestro Pro™ MEA system (Axion Biosystems). (A - D) Detected spikes (black lines), single channel bursts (blue lines; a collection of at least 5 spikes, each separated by an ISI of no more than 100 ms), and network bursts (orange boxes; a collection of at least 50 spikes from a minimum of 35% of participating electrodes, each separated by an ISI of no more than 100 ms) were recorded for each timepoint. Neuronal activity can be detected by Day 14 and increases over time throughout the 42-day culture period. (E) Mean firing rate, (F) number of bursts, and (G) synchrony index were all shown to increase over the 42-day culture period. hiPSC = human induced pluripotent stem cell; MEA = microelectrode array; ISI = inter-spike interval

    Protocols and Documentation

    Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

    Document Type
    Product Name
    Catalog #
    Lot #
    Language
    Document Type
    Product Information Sheet
    Product Name
    Human iPSC-Derived Forebrain Neuron Precursor Cells
    Catalog #
    200-0770
    Lot #
    All
    Language
    English

    Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    Research Area
    Workflow Stages
    Legal Statement: These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) shall not be used or administered in (1) human subjects for human clinical use; (2) animals for veterinary use for therapeutic, diagnostic, or prophylactic purposes or (3) any subject in relation to clinical applications, cell therapy, transplantation, and/or regenerative medicines, without limiting the generality of the foregoing. These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for monetization or commercialization purposes, including without limitation, used to, or with the goal to, perform services or supply products or rights, including in the manufacture of cellular therapies or other therapeutics, for monetary gain or the generation of royalties, revenues, sales or other valuable consideration. For clarity, these iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for screening compounds, antibodies, proteins or peptides, except for the purposes of target discovery, target validation, or assay development, provided such activities and the results of such activities are not further used for monetization or commercialization purposes. It may be possible to obtain a further license for the prohibited uses referred to in this Limited Use License. Please contact iPSCrequests@stemcell.com for more details. UNLESS OTHERWISE STATED, PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES. FOR PRODUCT-SPECIFIC COMPLIANCE AND INTENDED USE INFORMATION, REFER TO THE PRODUCT INFORMATION SHEET. GENERAL INFORMATION ON QUALITY AT STEMCELL MAY BE FOUND AT WWW.STEMCELL.COM/COMPLIANCE.
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