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人iPSC衍生的前脑神经元前体细胞

人诱导多能干细胞系SCTi003-A分化的冻存的前脑神经元前体细胞
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产品号 #200-0770_C

人诱导多能干细胞系SCTi003-A分化的冻存的前脑神经元前体细胞

产品优势

  • 采用混合的兴奋性和抑制性前脑神经元前体细胞加速您的研究进程
  • 通过经充分鉴定的iPSC标准细胞系SCTi003-A分化获得高品质神经元
  • 通过验证神经元电生理活性可证实其生理相关性强
  • 结合STEMdiff™前脑神经元成熟试剂盒使用以实现神经元的长期培养
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  1. STEMdiff™ Forebrain Neuron Maturation Kit
    STEMdiff™ Forebrain Neuron Maturation Kit

    Maturation kit for generation of functional neurons from human ES and iPS cell-derived neuronal precursor cells

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相关材料与文献
选择即用型、高纯度的前脑神经元前体细胞开启您的神经研究流程，加速获得更好的实验数据。本产品为混合的兴奋性与抑制性神经元细胞群，复苏后仅需两周即可获得功能活跃的iPSC衍生成熟神经元，为您提供生理相关性强的即用型研究模型。

为了确保您的神经研究的最佳质量，这些人iPSC衍生的前脑神经元前体细胞使用STEMdiff™ SMADi神经诱导试剂盒和STEMdiff™前脑神经元分化试剂盒，通过人iPSC标准细胞系SCTi003-A制备。结合STEMdiff™前脑神经元成熟试剂盒，可实现神经元前体细胞的长期培养与成熟。该细胞是研究人类神经发育与疾病、药物筛选、毒性测试及细胞疗法验证的多功能工具。

对于细胞间相互作用的体外高级模型，这些神经元也可以与以下STEMdiff™ hPSC分化试剂盒生成的细胞共培养，例如：
• STEMdiff™ 星形胶质细胞分化试剂盒
• STEMdiff™ 小胶质细胞分化试剂盒

更多详细信息请浏览iPSCs常见问题解答页面。
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Data Figures

Phase contrast image of Human iPSC-Derived Forebrain Neuron Precursor Cells exhibiting high-quality morphology at various time points.

Figure 1. Human iPSC-Derived Forebrain Neuron Precursor Cells Exhibit High-Quality Morphology

Cryopreserved Human iPSC-Derived Forebrain Neuron Precursor Cells were generated from SCTi003-A iPSCs were thawed and plated onto PLO/Laminin-coated plates at 80,000 cells/cm2 in STEMdiff™ Forebrain Neuron Maturation Kit. Neurons were incubated at 37°C and subsequently analyzed by brightfield microscopy at 10x magnification. Forebrain neurons display the expected neuronal morphology with minimal clumping over long-term culture. iPSC = induced pluripotent stem cell

Immunocytochemistry image of Day 19 neurons matured from Human iPSC-Derived Forebrain Neuron Precursor Cells exhibiting a highly pure βIII-TUB-positive neuronal population.

Figure 2. Neurons Matured from Human iPSC-Derived Forebrain Neuron Precursor Cells Consist of a Highly Pure Neuronal Population

Human iPSC-Derived Neuron Precursor Cells generated from SCTi003-A iPSCs were thawed, established in culture, and fixed for immunocytochemistry. (A) Day 14 neurons express high levels of neuronal marker βIII-TUB (red) with low expression of S100B (green). In addition, they display the typical neuronal morphology with healthy neurites and minimal cell clumping. (B) The percentage expression of these markers were quantified. Neuronal marker βIII-TUB was found to be expressed in 95% of neurons, while glial marker S100B was expressed in less than 4% of neurons. Error bars represent standard deviation (n = 2 biological replicates). iPSC = induced pluripotent stem cell

Immunocytochemistry image of Day 24 neurons matured from Human iPSC-Derived Forebrain Neuron Precursor Cells exhibiting MAP2, GABA, FOXG1 and Synapsin markers.

Figure 3. Neurons Matured from Human iPSC-Derived Forebrain Neuron Precursor Cells Express Characteristic Neuronal Markers

Human iPSC-Derived Neuron Precursor Cells generated from SCTi003-A iPSCs were thawed, established in culture, and fixed for immunocytochemistry. Day 24 neurons express neuronal marker MAP2, (A) GABA, (B) forebrain neuron marker FOXG1, and (C) pre-synaptic marker Synapsin. In addition, they display the typical neuronal morphology with healthy neurites and minimal cell clumping. iPSC = induced pluripotent stem cell

Bulk RNA-seq heatmap showing that Human iPSC-Derived Forebrain Neuron Precursor Cells express expected markers at various time points.

Figure 4. Human iPSC-Derived Forebrain Neuron Precursor Cells Express Expected Neuronal Markers

Human iPSC-Derived Forebrain Neuron Precursor Cells were generated from iPS cell line SCTi003-A. The neuron precursors were then matured with STEMdiff™ Forebrain Neuron Maturation Kit. RNA from forebrain neurons was harvested at various time points during maturation (Day 0, Day 14, and Day 21 in maturation media). This RNA from forebrain neurons along with RNA from the parent hPSC control were subsequently sequenced using bulk RNA-seq. The heat map displays expression levels for select genes associated with hPSCs, NPCs or forebrain neuron precursors, and forebrain neurons. iPS = induced pluripotent stem; hPSC = human pluripotent stem cell; NPC = neural progenitor cell

Electrical activity of Human iPSC-Derived Forebrain Neuron Precursor Cells were measured using the Maestro Pro™ MEA system from Axio Biosystems

Figure 5. Human iPSC-Derived Forebrain Neuron Precursor Cells Increase Neuronal Activity Over 42 Days in Culture

Human iPSC-Derived Forebrain Neuron Precursor Cells were generated from the hiPSC line SCTi003-A (Catalog #200-0511). The neuron precursors were then matured on a 48-well MEA plate (Axion Biosystems) with STEMdiff™ Forebrain Neuron Maturation Kit (Catalog #08605). Electrical activity from 16 electrodes was measured over time using the Maestro Pro™ MEA system (Axion Biosystems). (A - D) Detected spikes (black lines), single channel bursts (blue lines; a collection of at least 5 spikes, each separated by an ISI of no more than 100 ms), and network bursts (orange boxes; a collection of at least 50 spikes from a minimum of 35% of participating electrodes, each separated by an ISI of no more than 100 ms) were recorded for each timepoint. Neuronal activity can be detected by Day 14 and increases over time throughout the 42-day culture period. (E) Mean firing rate, (F) number of bursts, and (G) synchrony index were all shown to increase over the 42-day culture period. hiPSC = human induced pluripotent stem cell; MEA = microelectrode array; ISI = inter-spike interval

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Human iPSC-Derived Forebrain Neuron Precursor Cells
Catalog #
200-0770
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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Species Human
Contains CryoStor® CS10
Cell And Tissue Source Pluripotent Stem Cells
Donor Status Normal
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