Culture medium kit for establishment of organoid models containing human pluripotent stem cell-derived choroid plexus-like epithelium
产品优势
Adopt a relevant human CNS barrier model for screening and modeling applications with the precise control of an in vitro system
Eliminate the need for complex co-cultures or Transwell® systems in your screening pipeline
Extract cerebrospinal-like fluid to identify human CNS-specific biomarkers in a 100% blood-free system, without requiring a lumbar puncture
Obtain 50X more cerebrospinal-like fluid from a single kit than obtained from a single mouse, and eliminate the donor-to-donor variability of pooled clinical samples
Take an in vitro approach to human neural biomarker discovery and central nervous system (CNS) permeability with human pluripotent stem cell (hPSC)-derived organoids patterned to the choroid plexus, the specialized brain epithelium that forms the blood-cerebrospinal fluid barrier. With a three-dimensional tissue structure and functional barrier epithelium, you’ll achieve unprecedented access and control in studying CNS permeability for drug distribution studies, as well as for production and extraction of cerebrospinal fluid (CSF).
Using STEMdiff™ Choroid Plexus Differentiation Kit’s simple five-stage protocol, you can efficiently and reproducibly generate choroid plexus organoids for a variety of downstream applications. The kit is based on the formulation published by Pellegrini et al. (Science, 2020), which was awarded a 3Rs Prize for its breakthroughs in animal reduction, replacement, and refinement. Choroid plexus organoid formation is initiated through an intermediate embryoid body (EB) formation step followed by expansion of neuroepithelia and patterning to choroid plexus-like epithelium. After a maturation period, organoids generated using this kit feature cystic structures filled with a fluid resembling CSF and surrounded by an epithelial layer expressing ependymal choroid plexus-specific markers (TTR, CLIC6, AQP1).
For extended periods of organoid culture (> 40 days), the components required for organoid maturation can be purchased as STEMdiff™ Choroid Plexus Organoid Maturation Kit (Catalog #100-0825).
Cell Type Epithelial Cells, PSC-Derived, Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Pluripotent Stem Cells
Area of Interest Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Extracellular Vesicle Research, Neuroscience, Organoids
Formulation Category Serum-Free
Data Figures
Figure 1. Protocol Schematic for the STEMdiff™ Choroid Plexus Organoid Differentiation and Maturation Kits
Choroid plexus organoids can be generated from human embryonic stem (ES) or induced pluripotent stem (iPS) cells in 30 days. The protocol begins with embryoid body (EB) formation, followed by expansion of neuroepithelia and patterning to choroid plexus-like epithelium. After a period of epithelial maturation including extensive bubbling, the organoids develop cystic structures surrounded by an ependymal epithelial layer and filled with a fluid resembling cerebrospinal fluid (CSF).
Figure 2. Organoids Generated Using the STEMdiff™ Choroid Plexus Organoid Differentiation and Maturation Kits Exhibit Fluid-Filled Cysts and Ependymal Epithelial Morphology
(A) Phase-contrast image of a whole choroid plexus organoid at day 40 using the STEMdiff™ Choroid Plexus Organoid Differentiation and Maturation Kits. Organoids exhibit translucent cysts containing a CSF-like fluid. (B) Epithelia on choroid plexus organoids exhibit a tightly packed and cuboidal morphology typical of ependymal cells that line the brain ventricles in vivo.
Figure 3. Choroid Plexus Organoids Express Markers of Ependymal Epithelium and Show Limited Expression of Neuroepithelial Markers
Immunohistological analysis on whole cleared and stained (Masselink et al. 2019) choroid plexus organoids at day 40 reveals that their cystic structures are (A) TTR- (green) and (B) CLIC6- (red) positive. (C) These ependymal cell markers are highly co-localized. (D) Bundles of MAP2-positive (magenta) neurons are observed but are not co-localized with the ependymal cell markers.
Figure 4. Fluid Extracted from Cysts in Choroid Plexus Organoids is Enriched with Clusterin Protein, A Marker of Cerebrospinal Fluid (CSF)
CSF-like fluid was extracted from cysts contained in day 40 choroid plexus organoids using a 28G syringe. A western blot was performed on the extracted fluid to detect Clusterin and shows a band between the 37 and 50 kDa molecular weight marker. Clusterin is a soluble secreted chaperone protein found in high abundance in CSF.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they
can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
By submitting this form, you are providing your consent to STEMCELL Technologies Canada Inc. and its subsidiaries and affiliates (“STEMCELL”) to collect and use your information, and send you newsletters and emails in accordance with our
privacy policy. Please contact us with any questions that you may have. You can unsubscribe or change your email preferences at any time.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
