切换导航
  • 比较产品
我的购物车

STEMdiff™ 脉络丛类器官成熟试剂盒

用于建立包含人多能干细胞衍生的脉络丛样上皮的类器官模型的培养基试剂盒
率先评论此产品
添加并比较

产品号 #（选择产品）

产品号 #100-0825_C

用于建立包含人多能干细胞衍生的脉络丛样上皮的类器官模型的培养基试剂盒

产品优势

  • 采用相关的人类中枢神经系统屏障模型，通过精确控制体外系统进行筛选和建模应用
  • 无需在筛选流程中使用复杂的共培养或 Transwell® 系统
  • 提取类脑脊液，在不需要腰椎穿刺的情况下，在100%无血液的系统中识别特定于人类CNS的生物标志物
  • 从单个试剂盒中提取的类脑脊液是从单只小鼠中提取量的50倍，并消除了临床样本池中供体间的变异性

产品组分包括

  • STEMdiff™ 神经类器官基础培养基 4，250 mL
  • STEMdiff™ 神经类器官补充剂 I，4.5 mL
main product photo
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献

总览

利用人类多能干细胞 (hPSC) 衍生的类器官，以脉络丛（构成血脑脊液屏障的特殊脑上皮）为模型，采用体外方法探索人类神经生物标志物并测定中枢神经系统 (CNS) 的通透性。通过其三维组织结构和功能性屏障上皮，您将获得前所未有的可及性和可控性，用于药物分布研究以及脑脊液 (CSF) 的制备和提取。

使用 STEMdiff™ 脉络丛分化试剂盒的简易五阶段方案，您可以高效且可重复地生成脉络丛类器官，用于各种下游应用。该试剂盒基于 Pellegrini 等人发表的配方（Science，2020），该配方因其在动物减少、替代和精炼方面的突破而荣获3Rs 奖。脉络丛类器官的形成始于一个中间拟胚体 (EB) 形成步骤，随后神经上皮细胞扩增并逐渐形成脉络丛样上皮。经过一段成熟期后，使用该试剂盒生成的类器官呈囊性结构，其内部充满类似脑脊液的液体，并被一层表达脑室上皮脉络丛特异性标志物（TTR、CLIC6、AQP1）的上皮层包裹。

如需长时间培养（> 40 天），可购买 STEMdiff™ 脉络丛类器官成熟试剂盒（货号 100-0825）来获取类器官成熟所需的成分。

细胞类型
上皮细胞，PSC衍生，神经细胞，PSC衍生，神经干/祖细胞，多能干细胞
 
种属
人
 
应用
细胞培养，分化，功能学筛选，类器官培养，表型鉴定，细胞毒性检测
 
品牌
STEMdiff
 
研究领域
疾病建模，药物发现和毒理检测，上皮细胞研究，细胞外囊泡研究，神经科学，类器官
 
制剂类别
无血清
 

查看更多 显示更少

Data Figures

Cell culture protocol diagram to generate hPSC-derived choroid plexus organoids

Figure 1. Protocol Schematic for the STEMdiff™ Choroid Plexus Organoid Differentiation and Maturation Kits

Choroid plexus organoids can be generated from human embryonic stem (ES) or induced pluripotent stem (iPS) cells in 30 days. The protocol begins with embryoid body (EB) formation, followed by expansion of neuroepithelia and patterning to choroid plexus-like epithelium. After a period of epithelial maturation including extensive bubbling, the organoids develop cystic structures surrounded by an ependymal epithelial layer and filled with a fluid resembling cerebrospinal fluid (CSF).

Brightfield phase-contrast images of whole choroid plexus organoid and of cuboidal epithelial morphology

Figure 2. Organoids Generated Using the STEMdiff™ Choroid Plexus Organoid Differentiation and Maturation Kits Exhibit Fluid-Filled Cysts and Ependymal Epithelial Morphology

(A) Phase-contrast image of a whole choroid plexus organoid at day 40 using the STEMdiff™ Choroid Plexus Organoid Differentiation and Maturation Kits. Organoids exhibit translucent cysts containing a CSF-like fluid. (B) Epithelia on choroid plexus organoids exhibit a tightly packed and cuboidal morphology typical of ependymal cells that line the brain ventricles in vivo.

Immunostained choroid plexus organoids showing markers of ependymal epithelium TTR and CLIC6, with separate bundles of MAP2-positive neurons

Figure 3. Choroid Plexus Organoids Express Markers of Ependymal Epithelium and Show Limited Expression of Neuroepithelial Markers

Immunohistological analysis on whole cleared and stained (Masselink et al. 2019) choroid plexus organoids at day 40 reveals that their cystic structures are (A) TTR- (green) and (B) CLIC6- (red) positive. (C) These ependymal cell markers are highly co-localized. (D) Bundles of MAP2-positive (magenta) neurons are observed but are not co-localized with the ependymal cell markers.

Western blot with 2 columns showing a band in extracted CSF between the 37 and 50 kDa molecular weight markers, but not in spent culture media

Figure 4. Fluid Extracted from Cysts in Choroid Plexus Organoids is Enriched with Clusterin Protein, A Marker of Cerebrospinal Fluid (CSF)

CSF-like fluid was extracted from cysts contained in day 40 choroid plexus organoids using a 28G syringe. A western blot was performed on the extracted fluid to detect Clusterin and shows a band between the 37 and 50 kDa molecular weight marker. Clusterin is a soluble secreted chaperone protein found in high abundance in CSF.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
STEMdiff Choroid Plexus Organoid Maturation Kit
Catalog #
100-0825
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff Choroid Plexus Organoid Maturation Kit
Catalog #
100-0825
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff Choroid Plexus Organoid Maturation Kit
Catalog #
100-0825
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Workflow Stages for Organoids
View All

更多信息

更多信息
Species Human
Formulation Category Serum-Free
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
版权 © 2025 STEMCELL Technologies 技术有限公司。保留所有权利。