产品号 #78151_C
Nerve growth factor-beta, animal component-free
概述
Nerve growth factor (NGF)-beta is a prototypical member of the neurotrophin family and has a role in the survival and growth of neural cells, regulating cell growth, promoting differentiation into neurons, and neuron migration. The beta subtype of NGF is biologically active in comparison to the alpha-2 and gamma-2 subtypes. NGF-beta in its secreted form can bind to tyrosine kinase A (trkA) receptor with high affinity and to p75 (NTR) with low affinity (Levi & Alemà; Sofroniew et al.). NGF has been shown to possess pro-inflammatory and pro-fibrogenic properties (Micera et al.). It has also been shown that overexpression of NGF-beta promotes differentiation of bone marrow mesenchymal stem cells into neurons through regulation of AKT and MAPK pathways (Yuan et al.). This product is animal component-free.
Subtype
Cytokines, Growth Factors
Alternative Names
Beta-nerve growth factor, beta-NGF, nerve growth factor (beta polypeptide)
Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons
Species
Human
Area of Interest
Immunology, Stem Cell Biology
Formulation Category
Animal Component-Free
Molecular Weight
13.6 kDa
Purity
≥ 95%
Data Figures
(A) The biological activity of Human Recombinant NGF-beta, ACF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured after 60 hours in culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 1.31 ng/mL. (B) 1 μg of Human Recombinant NGF-beta, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Human Recombinant NGF-beta, ACF Catalog #
78151.2, 78151.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant NGF-beta, ACF Catalog #
78151.2, 78151.1 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Resources and Publications
Educational Materials (3)
Request Pricing
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
By submitting this form, you are providing your consent to STEMCELL Technologies Canada Inc. and its subsidiaries and affiliates (“STEMCELL”) to collect and use your information, and send you newsletters and emails in accordance with our
privacy policy. Please contact us with any questions that you may have. You can unsubscribe or change your email preferences at any time.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
欢迎您关注STEMCELL Technologies的微信公众平台
扫描二维码或搜索微信号STEMCELLTech，即可关注我们的微信平台，第一时间接收丰富的技术资源和最新的活动信息。
如您有任何问题，欢迎发消息给STEMCELLTech微信公众平台，或与我们通过电话/邮件联系：400 885 9050 INFO.CN@STEMCELL.COM。