产品号 #78065_C
Sonic hedgehog
概述
Sonic hedgehog (Shh) is a member of the Hedgehog family of secreted signaling proteins that has a prominent role in patterning during early mammalian development (Ho & Scott). It is expressed during early embryogenesis in a variety of tissues (Finco et al.). During patterning of the central nervous system, Shh directly acts on cells to specify neural cell fates, regulates proliferation and survival of oligodendrocytes precursors and neural crest cells and stimulates proliferation, differentiation and axon growth (Ho & Scott; Martí). Shh also regulates T cell differentiation and activation (Crompton et al.). Recombinant human Shh (C24II; rhShh) is a fully biologically active molecule.
Subtype
Cytokines
Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Pluripotent Stem Cells
Species
Human
Area of Interest
Neuroscience, Stem Cell Biology
Purity
≥ 95%
Data Figures
(A) The biological activity of Human Recombinant Shh (C24II) was tested by its ability to promote alkaline phosphatase production of CCL-226 cells. Alkaline phosphatase production was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example 0.7 μg/mL.
(B) 4 μg of Human Recombinant Shh (C24II) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant Shh (C24II) has a predicted molecular mass of 19.7 kDa.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Human Recombinant Shh (C24II) Catalog #
78065.2, 78065, 78065.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant Shh (C24II) Catalog #
78065.2, 78065, 78065.1 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Human Pluripotent Stem Cell Research
Resources and Publications
Educational Materials (1)
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