产品号 #60048_C
抗人、小鼠、大鼠胶质纤维酸性蛋白（GFAP）的小鼠单克隆IgG2b抗体
总览
2E1.E9抗体可与胶质纤维酸性蛋白 (GFAP) 发生反应。GFAP是一种约 49 kDa的 III 型中间丝 (IF) 蛋白，在中枢神经系统中主要由星形胶质细胞表达，但在某些胶质细胞衍生的肿瘤中也发现其含量较高。GFAP被认为有助于细胞骨架的结构和强度。2E1.E9抗体不会与其他 IF 蛋白发生交叉反应，可用于区分星形胶质细胞与其他胶质细胞。GFAP也已在间质细胞、角质形成细胞、软骨细胞和骨细胞中被发现。GFAP多肽包含一个 N 端头部、一个中央杆状结构和一个 C 端尾部结构域，并通过依赖于 N 端结构域磷酸化和去磷酸化的过程组装成二聚体。目前已鉴定出几种剪接变体，编码三种不同的亚型。 GFAP基因的多种突变（>50）与亚历山大症 (Alexander disease) 有关，这是一种进行性白质脑病，其特征是细胞质内含体和髓鞘形成功能障碍。该抗体克隆已验证可用于标记在NeuroCult™ NS-A扩增试剂盒（人；产品号 #05751）和NeuroCult™扩增试剂盒（小鼠；产品号 #05702）中生长的神经干细胞和祖细胞。
亚型
一抗
靶抗原
GFAP (Glial Fibrillary Acidic Protein)
别名
胶质纤维酸性蛋白（GFAP）
活性物种
人，小鼠，大鼠
偶联
未偶联的
宿主物种
小鼠
细胞类型
星形胶质细胞，神经细胞，PSC衍生
种属
人，小鼠，大鼠
应用
流式细胞术，免疫细胞化学，免疫荧光，免疫组化，Western印迹
研究领域
神经科学
克隆
2E1.E9
基因编号
2670
同种型
IgG2b
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (2)
Publications (1)
Onset of rosette formation during spontaneous neural differentiation of hESC and hiPSC colonies Gene 2014 JAN
Abstract
In vitro neural differentiation of human embryonic stem cells (hESCs) is an advantageous system for studying early neural development. The process of early neural differentiation in hESCs begins by initiation of primitive neuroectoderm, which is manifested by rosette formation, with consecutive differentiation into neural progenitors and early glial-like cells. In this study, we examined the involvement of early neural markers - OTX2, PAX6, Sox1, Nestin, NR2F1, NR2F2, and IRX2 - in the onset of rosette formation, during spontaneous neural differentiation of hESC and human induced pluripotent stem cell (hiPSC) colonies. This is in contrast to the conventional way of studying rosette formation, which involves induction of neuronal differentiation and the utilization of embryoid bodies. Here we show that OTX2 is highly expressed at the onset of rosette formation, when rosettes comprise no more than 3-5 cells, and that its expression precedes that of established markers of early neuronal differentiation. Importantly, the rise of OTX2 expression in these cells coincides with the down-regulation of the pluripotency marker OCT4. Lastly, we show that cells derived from rosettes that emerge during spontaneous differentiation of hESCs or hiPSCs are capable of differentiating into dopaminergic neurons in vitro, and into mature-appearing pyramidal and serotonergic neurons weeks after being injected into the motor cortex of NOD-SCID mice. ?? 2013 Elsevier B.V.
更多信息
| Species | Human, Mouse, Rat |
|---|---|
| Clone | 2E1.E9 |
| Gene Id | 2670 |
| Alternative Names | Glial fibrillary acid protein (GFAP) |
| Isotype | IgG2b |
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
