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STEMdiff™中脑神经元成熟试剂盒

从人胚胎干细胞和iPS细胞衍生的神经元前体细胞中生成功能性中脑型神经元的成熟试剂盒
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产品号 #（选择产品）

产品号 #100-0041_C

从人胚胎干细胞和iPS细胞衍生的神经元前体细胞中生成功能性中脑型神经元的成熟试剂盒

产品优势

  • 明确且无血清
  • 支持高效生成中脑样功能神经元从hpsc衍生的神经元前体
  • 产生可在培养中长期维持的th阳性神经元群（> - 15%）
  • STEMdiff™中脑神经元分化试剂盒优化了中脑神经元前体的成熟
  • 支持神经元活动的生理学相关结果
  • 使来自多种人类胚胎干细胞和iPS细胞系的中脑型神经元前体可再生成熟

产品组分包括

  • STEMdiff™中脑神经元成熟补充液，25 mL
  • BrainPhys™神经元培养基，100 mL
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Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. ACCUTASE™
    ACCUTASE™

    Cell detachment solution

  2. STEMdiff™ SMADi Neural Induction Kit
    STEMdiff™ SMADi Neural Induction Kit

    Serum-free medium kit for highly efficient SMAD inhibition-mediated neural induction of human ES and iPS cells

  3. STEMdiff™ Midbrain Neuron Differentiation Kit
    STEMdiff™ Midbrain Neuron Differentiation K...

    Differentiation kit for the generation of midbrain-patterned neuronal precursors from human ES and iPS cell-derived neural progenitor cells

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实验数据
产品说明书及文档
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概述

STEMdiff™中脑神经元成熟试剂盒（目录#100-0041）与STEMdiff™中脑神经元分化试剂盒（目录#100-0038）一起使用，从人类多能干细胞衍生的神经祖细胞中产生混合的中脑型神经元群体。这些培养基将产生大量中脑神经元(≥15% th阳性多巴胺能神经元；ⅲ类β微管蛋白阳性神经元≥90%；< 10% gmap阳性星形胶质细胞)。使用这些产品衍生的细胞是模拟人类神经发育和疾病、药物筛选、毒性测试和细胞治疗验证的通用工具。

Subtype
Specialized Media
 
Cell Type
Dopaminergic Neurons, Neural Cells, PSC-Derived, Neurons
 
Species
Human
 
Application
Cell Culture, Characterization, Differentiation, Functional Assay, Immunocytochemistry, Phenotyping
 
Brand
STEMdiff
 
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience
 
Formulation Category
Serum-Free
 

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Data Figures

Experimental Protocol for STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits (Embryoid Body Protocol)

Figure 1. Schematic for the Embryoid Body Protocol

Midbrain-type neurons, including dopaminergic neurons, can be generated from neural precursors after 12 days in STEMdiff™ Midbrain Neuron Differentiation Medium. For differentiation of precursors from embryonic and induced pluripotent stem cells, see the PIS.

Experimental Protocol for STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits (Monolayer Protocol)

Figure 2. Schematic for the Monolayer Protocol

Midbrain-type neurons, including dopaminergic neurons, can be generated from neural precursors after 6 days in STEMdiff™ Midbrain Neuron Differentiation Medium. For differentiation of precursors from embryonic and induced pluripotent stem cells, see the PIS.

Midbrain-Type Neurons Arise From Neural Progenitor Cells After Culture in STEMdiff™ SMADi Neural Induction Kit and STEMdiff™ Midbrain Culture System

Figure 3. Midbrain-Type Neurons Are Generated After Culture in STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits

NPCs generated from H1 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit embryoid body (EB) protocol were differentiated and matured to midbrain-type neurons using the STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits. (A) Midbrain-type neurons were formed after hPSC-derived NPCs were cultured with the STEMdiff™ Midbrain Neuron Differentiation Kit for 12 days and STEMdiff™ Midbrain Neuron Maturation Kit for 14 days. The resulting cultures contain a population of (B) class III β-tubulin-positive neurons (magenta), with (D) more than 15% Tyrosine Hydroxylase-positive cells (green). (C) Nuclei are labeled with DAPI (blue).

Figure 4. Midbrain-Type Neural Precursor Cells Express Characteristic Markers After Culture in STEMdiff™ Midbrain Neuron Differentiation Kit

NPCs generated from STiPS-F016 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit monolayer protocol were differentiated to midbrain-type neural precursors using STEMdiff™ Midbrain Neuron Differentiation Kit for 7 days. (A) Midbrain-type neural precursor cell cultures contain a population of (A) FOXA2-expressing cells (red) and (B) NKX2.1-expressing cells (green). Merge image in (E) showing the bottom half of the same FOV includes DAPI-labeled nuclei (blue) and shows non-overlapping marker expression. The resulting neural precursor cultures also express (C) OTX2 (red) and are negative for (D) central nervous system NPC marker PAX6 (green). Merge image in (F) showing the bottom half of the same FOV includes nuclei that are labeled with DAPI (blue).

Figure 5. Midbrain-Type Neurons Express Dopamine Transporters (DAT) After Differentiation and Maturation in STEMdiff™ Midbrain Neuron Kits

NPCs generated from H9 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit monolayer protocol were differentiated and matured to midbrain-type neurons using the STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits. (A) Midbrain-type neurons were formed after NPCs were cultured with the STEMdiff™ Midbrain Neuron Differentiation Kit for 12 days and STEMdiff™ Midbrain Neuron Maturation Kit for 14 days. The resulting cultures contain a population of (B) class III β-tubulin-positive neurons (magenta), which (C) express DAT in blue, and (E) Tyrosine Hydroxylase-positive cells (green). (D) Nuclei are labeled with DAPI (white).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Midbrain Neuron Maturation Kit
Catalog #
100-0041
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Midbrain Neuron Maturation Kit
Catalog #
100-0041
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Midbrain Neuron Maturation Kit
Catalog #
100-0041
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human
Formulation Category Serum-Free
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