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STEMdiff™中脑神经元分化试剂盒

分化试剂盒用于从人类胚胎干细胞和iPS细胞衍生的神经祖细胞中产生中脑模式的神经前体细胞
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产品号 #（选择产品）

产品号 #100-0038_C

分化试剂盒用于从人类胚胎干细胞和iPS细胞衍生的神经祖细胞中产生中脑模式的神经前体细胞

产品优势

  • 明确且无血清
  • 支持高效生成中脑样功能神经元从hpsc衍生的神经元前体
  • 产生可在培养中长期维持的th阳性神经元群（> - 15%）
  • STEMdiff™SMADi神经诱导试剂盒优化神经祖细胞分化
  • 能够从多种人类胚胎干细胞和iPS细胞系中再生产生中脑型神经元前体

产品组分包括

  • STEMdiff™中脑神经元分化基础培养基，80 mL
  • STEMdiff™中脑神经元分化补充液，20ml
main product photo
Need a high-quality cell source? Use the hiPSC SCTi003-A (female) control line, manufactured with mTeSR™ Plus.
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. ACCUTASE™
    ACCUTASE™

    Cell detachment solution

  2. STEMdiff™ SMADi Neural Induction Kit
    STEMdiff™ SMADi Neural Induction Kit

    Serum-free medium kit for highly efficient SMAD inhibition-mediated neural induction of human ES and iPS cells

  3. STEMdiff™ Midbrain Neuron Maturation Kit
    STEMdiff™ Midbrain Neuron Maturation Kit

    Maturation kit for generation of functional midbrain-type neurons from human ES and iPS cell-derived neuronal precursor cells

  4. Human Recombinant Shh (C24II)
    Human Recombinant Shh (C24II)

    Sonic hedgehog

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实验数据
产品说明书及文档
应用领域
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概述

STEMdiff™中脑神经元分化试剂盒（目录#100-0038）用于使用STEMdiff™SMADi神经诱导试剂盒（目录#08581）从人类多能干细胞（hPSCs）衍生的神经祖细胞（npc）产生中脑神经元前体。使用STEMdiff™中脑成熟试剂盒（目录#100-0041），中脑神经元前体进一步成熟为中脑神经元。这些培养基将产生大量中脑神经元(≥15% th阳性多巴胺能神经元；ⅲ类β微管蛋白阳性神经元≥90%；< 10% gmap阳性星形胶质细胞)。使用这些产品衍生的细胞是模拟人类神经发育和疾病、药物筛选、毒性测试和细胞治疗验证的通用工具。

Subtype
Specialized Media
 
Cell Type
Dopaminergic Neurons, Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells
 
Species
Human
 
Application
Cell Culture, Differentiation
 
Brand
STEMdiff
 
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience
 
Formulation Category
Serum-Free
 

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Data Figures

Experimental Protocol for STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits (Embryoid Body Protocol)

Figure 1. Schematic for the Embryoid Body Protocol

Midbrain-type neural precursors can be generated in 18 - 19 days from hPSC-derived neural progenitor cells (NPCs) after selecting neural rosettes from replated embryoid bodies. For the maturation of precursors to midbrain-type neurons, including dopaminergic neurons, see the PIS.

Experimental Protocol for STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits (Monolayer Protocol)

Figure 2. Schematic for the Monolayer Protocol

Midbrain-type neural precursors can be generated from neural progenitor cell (NPC) monolayers derived from embryonic and induced pluripotent stem cells after three single-cell passages. For the maturation of precursors to midbrain-type neurons, including dopaminergic neurons, see the PIS.

Midbrain-Type Neurons Arise From Neural Progenitor Cells After Culture in STEMdiff™ SMADi Neural Induction Kit and STEMdiff™ Midbrain Culture System

Figure 3. Midbrain-Type Neurons Are Generated After Culture in STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits

NPCs generated from H1 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit embryoid body (EB) protocol were differentiated and matured to midbrain-type neurons using the STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits. (A) Midbrain-type neurons were formed after hPSC-derived NPCs were cultured with the STEMdiff™ Midbrain Neuron Differentiation Kit for 12 days and STEMdiff™ Midbrain Neuron Maturation Kit for 14 days. The resulting cultures contain a population of (B) class III β-tubulin-positive neurons (magenta), with (D) more than 15% Tyrosine Hydroxylase-positive cells (green). (C) Nuclei are labeled with DAPI (blue).

Figure 4. Midbrain-Type Neural Precursor Cells Express Characteristic Markers After Culture in STEMdiff™ Midbrain Neuron Differentiation Kit

NPCs generated from STiPS-F016 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit monolayer protocol were differentiated to midbrain-type neural precursors using STEMdiff™ Midbrain Neuron Differentiation Kit for 7 days. (A) Midbrain-type neural precursor cell cultures contain a population of (A) FOXA2-expressing cells (red) and (B) NKX2.1-expressing cells (green). Merge image in (E) showing the bottom half of the same FOV includes DAPI-labeled nuclei (blue) and shows non-overlapping marker expression. The resulting neural precursor cultures also express (C) OTX2 (red) and are negative for (D) central nervous system NPC marker PAX6 (green). Merge image in (F) showing the bottom half of the same FOV includes nuclei that are labeled with DAPI (blue).

Figure 5. Midbrain-Type Neurons Express Dopamine Transporters (DAT) After Differentiation and Maturation in STEMdiff™ Midbrain Neuron Kits

NPCs generated from H9 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit monolayer protocol were differentiated and matured to midbrain-type neurons using the STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits. (A) Midbrain-type neurons were formed after NPCs were cultured with the STEMdiff™ Midbrain Neuron Differentiation Kit for 12 days and STEMdiff™ Midbrain Neuron Maturation Kit for 14 days. The resulting cultures contain a population of (B) class III β-tubulin-positive neurons (magenta), which (C) express DAT in blue, and (E) Tyrosine Hydroxylase-positive cells (green). (D) Nuclei are labeled with DAPI (white).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Midbrain Neuron Differentiation Kit
Catalog #
100-0038
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Midbrain Neuron Differentiation Kit
Catalog #
100-0038
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Midbrain Neuron Differentiation Kit
Catalog #
100-0038
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human
Formulation Category Serum-Free
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