产品号 #78005_C
脑源性神经营养因子
脑源性神经营养因子（BDNF）如神经生长因子（NGF）、神经营养因子-3 （NT-3）和神经营养因子-4 （NT-4）一样，是神经营养因子NGF家族的成员，是中枢和周围神经系统中特定神经元亚群分化和存活所必需的(Minichiello & Klein；Minichiello等人)。BDNF以高亲和力结合原肌球蛋白受体激酶B (TrkB)，激活AKT和ERK通路（Mattson等人）。它在基底前脑的海马体、皮质和突触中表达。BDNF作为人胚胎干细胞的存活因子，被镀于饲养细胞或康宁®Matrigel®（Pyle等人）。BDNF调节中枢神经系统成年突触的突触传递和可塑性，并参与适应性神经元反应，包括长期增强、长期抑制、某些形式的短期突触可塑性和神经元兴奋性的稳态调节（Reichardt）。它还通过促进背根神经节细胞、海马和皮质神经元的存活和生长而在神经发生中发挥作用（Binder & Scharfman）。BDNF 与胶质细胞源性神经营养因子（GDNF）及其他补充因子一起，通常用于将人多能干细胞（hPSC）衍生的神经前体细胞分化为神经元（Brafman）。
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (5)
Publications (1)
A Novel Protocol for Directed Differentiation of C9orf72-Associated Human Induced Pluripotent Stem Cells Into Contractile Skeletal Myotubes STEM CELLS Translational Medicine 2016 NOV
Abstract
: Induced pluripotent stem cells (iPSCs) offer an unlimited resource of cells to be used for the study of underlying molecular biology of disease, therapeutic drug screening, and transplant-based regenerative medicine. However, methods for the directed differentiation of skeletal muscle for these purposes remain scarce and incomplete. Here, we present a novel, small molecule-based protocol for the generation of multinucleated skeletal myotubes using eight independent iPSC lines. Through combinatorial inhibition of phosphoinositide 3-kinase (PI3K) and glycogen synthase kinase 3β (GSK3β) with addition of bone morphogenic protein 4 (BMP4) and fibroblast growth factor 2 (FGF2), we report up to 64% conversion of iPSCs into the myogenic program by day 36 as indicated by MYOG+ cell populations. These cells began to exhibit spontaneous contractions as early as 34 days in vitro in the presence of a serum-free medium formulation. We used this protocol to obtain iPSC-derived muscle cells from frontotemporal dementia (FTD) patients harboring C9orf72 hexanucleotide repeat expansions (rGGGGCC), sporadic FTD, and unaffected controls. iPSCs derived from rGGGGCC carriers contained RNA foci but did not vary in differentiation efficiency when compared to unaffected controls nor display mislocalized TDP-43 after as many as 120 days in vitro. This study presents a rapid, efficient, and transgene-free method for generating multinucleated skeletal myotubes from iPSCs and a resource for further modeling the role of skeletal muscle in amyotrophic lateral sclerosis and other motor neuron diseases. SIGNIFICANCE Protocols to produce skeletal myotubes for disease modeling or therapy are scarce and incomplete. The present study efficiently generates functional skeletal myotubes from human induced pluripotent stem cells using a small molecule-based approach. Using this strategy, terminal myogenic induction of up to 64% in 36 days and spontaneously contractile myotubes within 34 days were achieved. Myotubes derived from patients carrying the C9orf72 repeat expansion show no change in differentiation efficiency and normal TDP-43 localization after as many as 120 days in vitro when compared to unaffected controls. This study provides an efficient, novel protocol for the generation of skeletal myotubes from human induced pluripotent stem cells that may serve as a valuable tool in drug discovery and modeling of musculoskeletal and neuromuscular diseases.
更多信息
| Species | Human |
|---|---|
| Purity | ≥ 95% |
相关产品
-
NeuroCult™SM1神经元补充剂补充（50X）用于无血清培养神经元
-
BrainPhys™神经元介质改善神经功能的无血清神经生理基础培养基
-
N2补充用于小鼠和人胚胎干细胞和iPS细胞的神经和胰腺分化
-
人重组GDNF神经胶质细胞系衍生的神经营养因子
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
