产品号 #100-2062_C
Heregulin-beta 1, animal component-free
概述
Heregulin-beta 1 also known as neuregulin-1 (NRG-1) is a member of the epidermal growth factor (EGF) family of growth factors and acts as a ligand for ErbB family receptor tyrosine kinases (Britsch et al.). Heregulin/neuregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3, and NRG4). Heregulin-beta 1 plays an important role during the development of the nervous system, heart, and mammary glands (Britsch). Heregulin-beta 1 is expressed in neuronal cells, and modulates cell growth and differentiation of the cells during development and wound healing (Mei & Xiong). It has been implicated through in vivo and in vitro studies that heregulin-beta 1/ErbB signaling is crucial for multiple aspects of cardiovascular development and protects the heart from ischemic injury (Odiete et al.). Heregulin-beta 1 also promotes invasiveness and metastasis of breast cancer cells (Hutcheson et al.). It has also been shown that heregulin-beta 1 has a role in the growth and maintenance of human embryonic stem cells (Wang et al.). This product is animal component-free (ACF).
Subtype
Cytokines
Alternative Names
Acetylcholine receptor-inducing activity, ARIA, Breast cancer cell differentiation factor p45, Glial growth factor, Heregulin Neu differentiation factor, HRG, HRG1, HRG1-beta 1, Neuregulin-1, NRG1, NRG1-beta 1, Sensory and motor neuron-derived factor
Cell Type
Cancer Cells and Cell Lines, Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons
Species
Human, Other
Area of Interest
Cancer, Neuroscience, Stem Cell Biology
Formulation Category
Animal Component-Free
Purity
≥ 98% by SDS-PAGE
Data Figures
Figure 1. Biological Activity and Molecular Mass of Human Recombinant Heregulin-beta 1, ACF
(A) The biological activity of Human Recombinant Heregulin-beta 1, ACF was tested by its ability to induce MAP/ERK signaling in transfected MCF-7 cells using a luciferase reporter assay. Firefly luciferase activity is normalized to the control Renilla luciferase activity. The EC50 is defined as the effective concentration of the growth factor at which Heregulin-beta 1 response is at 50% of maximum. The EC50 in the above example is 37.9 pM (0.28 ng/mL).
(B) 3 μg of Human Recombinant Heregulin-beta 1, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant Heregulin-beta 1, ACF has a predicted molecular mass of 7.5 kDa.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Human Recombinant Heregulin-beta 1, ACF Catalog #
100-2062 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant Heregulin-beta 1, ACF Catalog #
100-2062 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Neuronal Research
Workflow Stages for hPSC-Derived Neural Cell Research
Workflow Stages for Human Pluripotent Stem Cell Research
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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