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人iPSC衍生的神经祖细胞

冻存的人神经祖细胞由人诱导多能干细胞系SCTi003-A分化而来
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产品号 #200-0620_C

冻存的人神经祖细胞由人诱导多能干细胞系SCTi003-A分化而来

产品优势

  • 复苏后可立即用STEMdiff™神经祖细胞培养基扩增
  • 节省时间，使用高度鉴定的神经祖细胞中间体开始您的分化工作流程
  • 可使用STEMdiff™神经系统分化为前脑神经元和/或星形胶质细胞
  • 确保与相同遗传背景下产生的神经元-星形胶质细胞共培养物具有生理相关性
    • 发
  • 获得高品质的NPCs，其来源于高度鉴定的对照细胞系SCTi003-A

产品组分包括

  • 人iPSC衍生的神经祖细胞（产品号 #200-0620）
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Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. STEMdiff™ Neural Progenitor Medium
    STEMdiff™ Neural Progenitor Medium

    Medium for maintenance and expansion of neural progenitor cells derived from human ES and iPS cells

  2. STEMdiff™ Forebrain Neuron Differentiation Kit
    STEMdiff™ Forebrain Neuron Differentiation ...

    Differentiation kit for the generation of neuronal precursors from human ES and iPS cell-derived neural progenitor cells

  3. STEMdiff™ Astrocyte Differentiation Kit
    STEMdiff™ Astrocyte Differentiation Kit

    Serum-free differentiation kit for generating astrocyte precursors from hPSC-derived neural progenitor cells

  4. STEMdiff™ Midbrain Neuron Differentiation Kit
    STEMdiff™ Midbrain Neuron Differentiation K...

    Differentiation kit for the generation of midbrain-patterned neuronal precursors from human ES and iPS cell-derived neural progenitor cells

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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使用高品质、即用型人神经祖细胞 (NPCs)，自信地开始您的神经研究工作流程。这些特殊冻存的中枢神经系统 (CNS) 型祖细胞由人诱导多能干细胞 (iPSC) 对照系SCTi003-A分化而来，该细胞系源自健康女性供体的外周血单分核细胞 (PBMCs)。这些人NPCs复苏后可直接使用，且具有多能性，适用于使用STEMdiff™试剂盒使其下游定制化地分化为各种中枢神经系统细胞类型，如前脑神经元, 中脑神经元,星形胶质细胞。

为了确保细胞的高质量，这些NPCs通过使用无血清的STEMdiff™SMADi神经诱导试剂盒从稳定且经过广泛测试的SCTi003-A系分化而来。NPCs可以使用STEMdiff™神经祖细胞培养基进行扩增，从而实现规模化生产并降低需要大量细胞的工作流程的成本。使用STEMdiff™神经祖细胞冻存液冻存扩增的神经祖细胞 (NPCs)，为您的实验计划提供灵活性。

本产品仅供研究使用 (RUO)，已获得学术和商业用途许可。血液样本的采集符合伦理，并遵循机构审查委员会 (IRB) 或其他监管机构批准的知情同意书和方案。有关供体详情和来源细胞库的细胞质量鉴定，请参阅本页的数据图。SCTi003-A源自αβ T细胞，并经过VDJ序列重排。SCTi003-A核型稳定，具有三谱系分化潜能，表达未分化的细胞标志物，并使用非整合重编程技术进行重编程。已在hPSCreg®注册以确保符合基于社会标准的伦理和生物学合规性。如需更多细节，请参阅特定批次的分析证书和关于iPSC细胞系的常见问题解答。
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Data Figures

Thawed and plated Human iPSC-Derived Neural Progenitor Cells exhibit high-quality, characteristic NPC morphology.

Figure 1. Human iPSC-Derived Neural Progenitor Cells Exhibit High-Quality Morphology Characteristic of Multipotent Central Nervous System Progenitor Cells

Cryopreserved Human iPSC-Derived Neural Progenitor Cells were thawed and plated onto Corning® Matrigel®-coated plates at 200,000 cells/cm². NPCs were incubated for 24 hours in STEMdiff™ Neural Progenitor Medium at 37℃ and subsequently analyzed by brightfield microscopy. NPCs display the small, teardrop-shaped morphology expected for NPCs. (A) 4X magnification, (B) 10X magnification. iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Immunocytochemistry images show Human iPSC-Derived Neural Progenitor Cells expressing neural progenitor markers SOX1 and PAX6, with low βIII-TUB expression.

Figure 2. Human iPSC-Derived Neural Progenitor Cells Express Characteristic Markers

Human iPSC-Derived Neural Progenitor Cells generated from SCTi003-A iPSCs were thawed, established in culture, and fixed for immunocytochemistry. The NPCs express neural progenitor markers (A) SOX1 and (B) PAX6 with low expression of (C) βIII-TUB. (D) In addition, they display the expected small, teardrop-shaped morphology. (E) The percentage expression of these markers were quantified. Neural progenitor markers PAX6 and SOX1 were found to be expressed in 90% of NPCs, while mature neuronal marker βIII-TUB was expressed in less than 10% of NPCs. Error bars represent standard deviation (n = 2 biological replicates). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Immunocytochemistry images show Human iPSC-Derived Neural Progenitor Cells differentiated to forebrain neurons expressing neural identity marker βIII-TUB.

Figure 3. Human iPSC-Derived Neural Progenitor Cells Can Effectively Differentiate into Forebrain Neurons

Forebrain neurons were generated from Human iPSC-Derived Neural Progenitor Cells using STEMdiff™ Forebrain Neuron Differentiation Medium. NPCs were cultured for six days at 37℃ in STEMdiff™ Forebrain Neuron Differentiation Medium. Cells were subsequently cultured in STEMdiff™ Forebrain Neuron Maturation medium for 14 days. Resulting cells were processed for immunocytochemistry. (A) The resulting forebrain neuron cultures contain a population of cells expressing (B) neuronal identity marker βIII-TUB (magenta), (C) but not astrocyte marker GFAP (green). (D) Nuclei are labeled with DAPI (gray). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Immunocytochemistry images show Human iPSC-Derived Neural Progenitor Cells differentiated to midbrain neurons expressing neural identity marker, βIII-TUB, and dopaminergic neuron marker, TH, but not astrocyte marker, GFAP.

Figure 4. Human iPSC-Derived Neural Progenitor Cells Can Effectively Differentiate into Midbrain Neurons

Midbrain neurons were generated from Human iPSC-Derived Neural Progenitor Cells using STEMdiff™ Midbrain Neuron Differentiation Medium. NPCs were cultured for six days at 37℃ in STEMdiff™ Midbrain Neuron Differentiation Medium. Cells were subsequently cultured in STEMdiff™ Midbrain Neuron Maturation medium for 14 days. Resulting cells were processed for immunocytochemistry. (A) The resulting midbrain neuron cultures contain a population of cells expressing (B) neuronal identity marker βIII-TUB (red) and (C) dopaminergic neuron marker TH (green), (D) but not astrocyte marker GFAP (magenta). (D) Nuclei are labeled with DAPI (blue). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Immunocytochemistry images show Human iPSC-Derived Neural Progenitor Cells differentiated to astrocytes expressing astrocyte identity marker S100β and GFAP, but not neuronal marker DCX.

Figure 5. Human iPSC-Derived Neural Progenitor Cells Can Effectively Differentiate into Astrocytes

Astrocytes were generated from Human iPSC-Derived Neural Progenitor Cells using STEMdiff™ Astrocyte Differentiation Medium. NPCs were cultured for 21 days at 37℃ in STEMdiff™ Astrocyte Differentiation Medium. Cells were subsequently cultured in STEMdiff™ Astrocyte Maturation medium for seven days. Resulting cells were processed for immunocytochemistry. (A) The resulting astrocyte cultures contain a population of cells expressing (B) astrocyte marker S100β (green) and (C) GFAP (red), but not neuronal marker DCX (magenta). Nuclei are labeled with DAPI (blue). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Bar graph showing expansion rate of Human iPSC-Derived Neural Progenitor Cells to 5 passages.

Figure 6. Human iPSC-Derived Neural Progenitor Cells Can Be Expanded for Multiple Passages

Human iPSC-Derived Neural Progenitor Cells were thawed and maintained in STEMdiff™ Neural Progenitor Medium at 37℃ and passaged roughly once every seven days for a total of five weeks. The average fold increase of NPCs was 2.8 ± 0.5 (mean ± SEM) per passage, demonstrating cells can be effectively expanded.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Human iPSC-Derived Neural Progenitor Cells
Catalog #
200-0620, 200-0621
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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Workflow Stages

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Species Human
Contains STEMdiff™ Neural Progenitor Freezing Medium
Cell And Tissue Source Pluripotent Stem Cells
Donor Status Normal
Legal Statement: These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) shall not be used or administered in (1) human subjects for human clinical use; (2) animals for veterinary use for therapeutic, diagnostic, or prophylactic purposes or (3) any subject in relation to clinical applications, cell therapy, transplantation, and/or regenerative medicines, without limiting the generality of the foregoing. These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for monetization or commercialization purposes, including without limitation, used to, or with the goal to, perform services or supply products or rights, including in the manufacture of cellular therapies or other therapeutics, for monetary gain or the generation of royalties, revenues, sales or other valuable consideration. For clarity, these iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for screening compounds, antibodies, proteins or peptides, except for the purposes of target discovery, target validation, or assay development, provided such activities and the results of such activities are not further used for monetization or commercialization purposes. It may be possible to obtain a further license for the prohibited uses referred to in this Limited Use License. Please contact iPSCrequests@stemcell.com for more details. UNLESS OTHERWISE STATED, PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES. FOR PRODUCT-SPECIFIC COMPLIANCE AND INTENDED USE INFORMATION, REFER TO THE PRODUCT INFORMATION SHEET. GENERAL INFORMATION ON QUALITY AT STEMCELL MAY BE FOUND AT WWW.STEMCELL.COM/COMPLIANCE.
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