hPSC衍生神经细胞研究

神经诱导后神经祖细胞 (NPC) 的扩增可产生更大的细胞池，用于下游应用。 查看更多 When conducting replicate or follow-up experiments, researchers can return to NPCs generated from the original neural induction procedure, removing a significant source of variability. STEMdiff™ Neural Progenitor Medium is optimized for the expansion of NPCs generated using STEMdiff™ SMADi Neural Induction Kit or STEMdiff™ Neural Induction Medium. While preserving multipotency, STEMdiff™ Neural Progenitor Medium minimizes spontaneous neuronal differentiation. NPCs can be frozen at any point following neural induction with reproducibly high recovery rates using STEMdiff™ Neural Progenitor Freezing Medium. Post-thaw, NPCs display healthy morphology, express NPC markers, and retain the potential to expand and differentiate into neurons and glia.

神经嵴诱导后，神经嵴细胞 (NCC) 也可适度扩增。对于难以分化的患者细胞系，可使用 EasySep™ 释放人 PSC 来源的神经嵴正选试剂盒 纯化 STEMdiff™ 神经嵴分化试剂盒 产生的细胞群。细胞可在 STEMdiff™ 神经嵴分化试剂盒中传代最多三次，并可在 CryoStor® CS10 中冻存，用于储存或扩增，以分化为周围神经系统的神经元或神经胶质细胞。对于下游分化为间充质细胞类型（例如软骨细胞、脂肪细胞或成骨细胞），NCC 可在 MesenCult™-ACF Plus 培养基 中扩增最多三次。