产品号 #100-0047_C
采用颗粒释放技术的免疫磁阳性选择试剂盒
采用颗粒释放技术的免疫磁阳性选择试剂盒
Magnet for column-free immunomagnetic separation
Cell separation buffer
Compatible antibodies for purity assessment of isolated cells
EasySep™Release Human PSC -derived Neural Crest Cell Positive Selection Kit旨在通过免疫磁阳性选择从人多能干细胞(PSC)来源的神经嵴细胞培养物中分离出CD271 +细胞。
用抗体和磁性颗粒标记所需细胞,并使用EasySep™磁铁无柱分离。不需要的细胞被简单地倒出,而需要的细胞留在管中。然后,从EasySep™分离的CD271+细胞中去除结合的磁性颗粒,这些细胞立即可用于下游应用。
使用EasySep™释放试剂盒分离细胞后,抗体复合物仍与细胞表面结合,并可能与Brilliant Violet™抗体偶联物、聚乙二醇修饰蛋白或其他化学相关配体相互作用。
Magnet Compatibility
Figure 1. Histograms of H9 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry
Starting with a single-cell suspension of H9 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).
Figure 2. Histograms of H7 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry
Starting with a single-cell suspension of H7 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).
Figure 3. Histograms of 1C cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry
Starting with a single-cell suspension of 1C cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Species | Human |
---|---|
Magnet Compatibility | • EasySep™ Magnet (Catalog #18000) |
Sample Source | Other |
Selection Method | Positive |
用于MSCs向软骨细胞分化的无动物成分培养基
用于体外诱导人MSC分化为成骨细胞
改善神经功能的无血清神经生理基础培养基
用于建立人造血干细胞来源的神经嵴细胞的细胞培养试剂盒
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