EasySep™释放人psc衍生的神经嵴细胞阳性选择试剂盒

采用颗粒释放技术的免疫磁阳性选择试剂盒

产品号 #(选择产品)

产品号 #100-0047_C

采用颗粒释放技术的免疫磁阳性选择试剂盒

产品优势

  • 高度纯化的人psc来源的神经嵴细胞在不到20分钟内分离
  • EasySep™releable RapidSpheres™免洗去除
  • 兼容下游差异化协议

产品组分包括

  • EasySep™释放人类psc衍生的神经嵴细胞阳性选择鸡尾酒,2 × 1.0 mL
  • EasySep™分离鸡尾酒增强剂,1.0 mL
  • EasySep™释放缓冲液(浓缩),3 × 1.0 mL
  • EasySep™可释放RapidSpheres™50201,2 x 1.0 mL

概述

EasySep™Release Human PSC -derived Neural Crest Cell Positive Selection Kit旨在通过免疫磁阳性选择从人多能干细胞(PSC)来源的神经嵴细胞培养物中分离出CD271 +细胞。

用抗体和磁性颗粒标记所需细胞,并使用EasySep™磁铁无柱分离。不需要的细胞被简单地倒出,而需要的细胞留在管中。然后,从EasySep™分离的CD271+细胞中去除结合的磁性颗粒,这些细胞立即可用于下游应用。

使用EasySep™释放试剂盒分离细胞后,抗体复合物仍与细胞表面结合,并可能与Brilliant Violet™抗体偶联物、聚乙二醇修饰蛋白或其他化学相关配体相互作用。

Magnet Compatibility

• EasySep™ Magnet (Catalog #18000)
 
Subtype
Cell Isolation Kits
 
Cell Type
Neural Cells, PSC-Derived
 
Species
Human
 
Sample Source
Other
 
Selection Method
Positive
 
Application
Cell Isolation
 
Brand
EasySep
 
Area of Interest
Disease Modeling, Neuroscience
 

Data Figures

A H9 cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 74.9% and 93.8% pure.

Figure 1. Histograms of H9 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of H9 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).

A H7 cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 47.3% and 90.8% pure.

Figure 2. Histograms of H7 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of H7 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).

A 1C cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 18.9% and 86.7% pure.

Figure 3. Histograms of 1C cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of 1C cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0047
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0047
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0047
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0047
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
100-0047
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

更多信息

更多信息
Species Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000)
Sample Source Other
Selection Method Positive
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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