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EasySep™小鼠Pan-DC富集试剂盒II

免疫磁珠负选试剂盒
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产品号 #（选择产品）

产品号 #19863_C

免疫磁珠负选试剂盒

产品优势

  • 操作简单、快捷，且无需分离柱
  • 纯度高达65%
  • 分选得到的细胞不带标记
  • 为高得率进行优化
  • 便于快速流式分选DC亚型

产品组分包括

  • EasySep™小鼠Pan-DC富集试剂盒（产品号 #19863）
    • EasySep™小鼠Pan-DC富集抗体混合物II，1 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001磁珠，1 mL
main product photo
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

EasySep™ 小鼠Pan-DC富集试剂盒 II 旨在通过免疫磁珠负选，从小鼠脾细胞中分离树突细胞（DCs）。在该EasySep™分选流程中，非目的细胞被抗体复合物与磁珠标记。通过EasySep™磁极将标记的细胞与未被标记的目的细胞分离，接着简单地将目的细胞倾倒或吸取至一个新的试管中。

本产品替代了 EasySep™ 小鼠Pan-DC富集试剂盒（产品号 #19763），可提高得率并实现更快速的细胞分选。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
树突状细胞（DCs）
 
种属
小鼠
 
样本来源
Spleen
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫，感染性疾病(传染病)
 

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Data Figures

Flow Cytometry Profile Results with EasySep™ Mouse Pan-DC Enrichment Kit II

Figure 1. Typical EasySep™ Mouse Pan-DC Enrichment Profile

Starting with mouse splenocytes, the dendritic cell content (CD11c+lineage-) of the isolated fraction is typically 57.3 ± 5.5% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final enriched fractions are 3.9% and 62.4%, respectively.

Reduce sort time using EasySep™ Mouse Pan-DC Enrichment Kit II

Figure 2. Pre-enrichment with EasySep™ Reduces Sorting Time

Starting with mouse splenocytes, DCs and DC subsets were analyzed in parallel from unenriched or EasySep™-enriched samples by flow cytometry. Each sample was run for an equivalent amount of time with a consistent flow rate and the data was used to extrapolate the time required to sort 1 million pan-DCs, conventional DCs (cDCs), or plamacytoid DCs (pDCs). In all cases, the required FACS times were reduced with EasySep™-enriched samples compared to unenriched samples (n = 2, SD).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

更多信息

更多信息
Species Mouse
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
Sample Source Spleen
Selection Method Negative
标记抗体
  1. 抗小鼠CD3e抗体，克隆145-2C11
    抗小鼠CD3e抗体，克隆145-2C11

    亚美尼亚仓鼠单克隆IgG1抗体，抗小鼠CD3e

  2. 抗小鼠Ly-6G抗体，克隆1A8
    抗小鼠Ly-6G抗体，克隆1A8

    大鼠单克隆IgG2a抗体，抗小鼠Ly-6G

  3. 抗小鼠TER119抗体，克隆TER-119
    抗小鼠TER119抗体，克隆TER-119

    抗小鼠TER119的大鼠（WI）单克隆IgG2b抗体

  4. 抗小鼠CD19抗体，克隆ID3
    抗小鼠CD19抗体，克隆ID3

    大鼠单克隆IgG2a抗体，抗小鼠CD19

  5. 抗小鼠F4/80抗体，克隆BM8
    抗小鼠F4/80抗体，克隆BM8

    大鼠单克隆IgG2a抗体，抗小鼠F4/80

  6. 抗小鼠NK1.1（CD161）抗体，克隆PK136
    抗小鼠NK1.1（CD161）抗体，克隆PK136

    抗小鼠 NK1.1 (CD161) 的 (C3H x BALB/c) F1 杂交单克隆IgG2a抗体

  7. 抗小鼠CD11c抗体，克隆N418
    抗小鼠CD11c抗体，克隆N418

    抗小鼠CD11c的仓鼠（亚美尼亚）单克隆IgG抗体

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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