The EasySep™ Mouse Pan-DC Enrichment Kit II is designed to isolate dendritic cells (DCs) from mouse splenocytes by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
This product replaces the EasySep™ Mouse Pan-DC Enrichment Kit (Catalog #19763), for improved yield and even faster cell isolations.
This product replaces the EasySep™ Mouse Pan-DC Enrichment Kit (Catalog #19763), for improved yield and even faster cell isolations.
Data Figures
Figure 1. Typical EasySep™ Mouse Pan-DC Enrichment Profile
Starting with mouse splenocytes, the dendritic cell content (CD11c+lineage-) of the isolated fraction is typically 57.3 ± 5.5% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final enriched fractions are 3.9% and 62.4%, respectively.
Figure 2. Pre-enrichment with EasySep™ Reduces Sorting Time
Starting with mouse splenocytes, DCs and DC subsets were analyzed in parallel from unenriched or EasySep™-enriched samples by flow cytometry. Each sample was run for an equivalent amount of time with a consistent flow rate and the data was used to extrapolate the time required to sort 1 million pan-DCs, conventional DCs (cDCs), or plamacytoid DCs (pDCs). In all cases, the required FACS times were reduced with EasySep™-enriched samples compared to unenriched samples (n = 2, SD).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863RF
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Educational Materials (6)
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