产品号 #60027_C
Rat monoclonal IgG2a antibody against mouse F4/80
概述
The BM8 antibody reacts with the F4/80 antigen (also termed Ly-71 in mouse and EMR-1 in human), an ~160 kDa transmembrane glycoprotein belonging to the EGF-TM7 family of G-protein-coupled receptors. F4/80 is considered a marker of choice for the identification of mature tissue macrophages, being broadly but variably expressed by this cell type in the liver (Kupffer cells), skin (Langerhans cells), bone marrow stroma, pancreas, thymus, spleen (red pulp), lung, and other tissues. It is also expressed by circulating monocytes, eosinophils and a subset of dendritic cells. F4/80 expression levels increase following activation of macrophages. F4/80 is reportedly the only macrophage marker suitable for distinguishing destructive from non-destructive inflammatory processes in the pancreas. The protein is thought to play a role in the generation of CD8+ regulatory T cells involved in immune tolerance.
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Monocyte Enrichment Kit (Catalog #19761) and EasySep™ Mouse CD11b Positive Selection Kit (Catalog #18770).
Subtype
Primary Antibodies
Target Antigen
F4/80
Alternative Names
EMR1, Ly71
Reactive Species
Mouse
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Rat
Cell Type
Monocytes, Other
Species
Mouse
Application
Cell Isolation, ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
BM8
Gene ID
13733
Isotype
IgG2a, kappa
Data Figures
Figure 1. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, Alexa Fluor® 488 and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse CD11b Positive Selection Kit and labeled with Anti-Mouse F4/80 Antibody, Clone BM8, Alexa Fluor® 488.
(C) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody and antimouse CD45 APC.
Figure 2. Data for PE-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, PE and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with a rat IgG2a, kappa PE isotype control antibody and anti-mouse CD45 APC.
Figure 3. Data for Unconjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, followed by a mouse anti-rat IgG2a antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.
Figure 4. Data for APC-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.
Figure 5. Data for Biotin-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.
Figure 6. Data for FITC-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.
Figure 7. Data for PerCP-Cy55-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, PerCP-Cy5.5 and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Mouse F4/80 Antibody, Clone BM8, PE Catalog #
60027PE, 60027PE.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse F4/80 Antibody, Clone BM8 Catalog #
60027 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse F4/80 Antibody, Clone BM8, Biotin Catalog #
60027BT, 60027BT.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse F4/80 Antibody, Clone BM8, FITC Catalog #
60027FI.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse F4/80 Antibody, Clone BM8, APC Catalog #
60027AZ.1, 60027AZ Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse F4/80 Antibody, Clone BM8, PerCP-Cy5.5 Catalog #
60027PS.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse F4/80 Antibody, Clone BM8, PE Catalog #
60027PE, 60027PE.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse F4/80 Antibody, Clone BM8 Catalog #
60027 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse F4/80 Antibody, Clone BM8, Biotin Catalog #
60027BT, 60027BT.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse F4/80 Antibody, Clone BM8, FITC Catalog #
60027FI.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse F4/80 Antibody, Clone BM8, APC Catalog #
60027AZ.1, 60027AZ Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse F4/80 Antibody, Clone BM8, PerCP-Cy5.5 Catalog #
60027PS.1 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Dendritic Cell Research
Resources and Publications
Educational Materials (3)
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Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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