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抗小鼠TER119抗体，克隆TER-119

抗小鼠TER119的大鼠（WI）单克隆IgG2b抗体
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产品号 #60033_C

抗小鼠TER119的大鼠（WI）单克隆IgG2b抗体

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

TER-119 单克隆抗体可识别小鼠 TER119（又称 Ly-76），这是一种分子量约为 52 kDa 的蛋白质，通常与糖蛋白 A（glycophorin A）相关联，存在于胚胎卵黄囊、胎儿和新生小鼠肝脏、成年骨髓、外周血及淋巴器官中的红系细胞表面。

TER119 是一种红系特异性标志物，在红系细胞从早期红系祖细胞（proerythroblasts）到成熟红细胞的整个分化阶段中均有表达，但不在红系集落形成单位（BFU-E，即爆裂形成单位红系细胞，或 CFU-E，即集落形成单位红系细胞）中表达。

TER-119 抗体是“lineage抗体混合物”中的组分，常用于检测或去除分化为造血系的细胞。在成年小鼠中，TER119 表达于约 20–25% 的骨髓细胞和约 2–3% 的脾细胞（splenocytes）中。

该抗体克隆已被验证可用于评估使用 EasySep™ 试剂盒分选获得的细胞的纯度，包括 EasySep™ 小鼠 CD4⁺ T 细胞分离试剂盒（产品号 #19852）。

亚型
一抗
 
靶抗原
TER119
 
别名
Ly-76，TER-119
 
活性物种
小鼠
 
偶联
Biotin 或 生物素，FITC，PE，PerCP-Cyanine5.5
 
宿主物种
大鼠
 
细胞类型
红系细胞，红细胞
 
种属
小鼠
 
应用
细胞分选，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
 
研究领域
免疫
 
克隆
TER-119
 
基因编号
104231
 
同种型
IgG2b，kappa
 

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Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, followed by goat antimouse IgG, FITC.
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with a rat IgG2b, kappa isotype control antibody followed by goat anti-mouse IgG, FITC.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, Alexa Fluor® 488 (filled histogram) or a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Image showing flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER-119 Antibody, Clone TER-119, Biotin followed by streptavidin (SAV) PE or a rat IgG2b, kappa isotype control antibody, Biotin followed by streptavidin (SAV) PE.

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER-119 Antibody, Clone TER-119, Biotin followed by streptavidin (SAV) PE (filled histogram) or a rat IgG2b, kappa isotype control antibody, Biotin followed by streptavidin (SAV) PE (solid line histogram).

Data for PE-Conjugated

Figure 4. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (solid line histogram).

Data for APC-Conjugated

Figure 5. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, Alexa Fluor® 488
Catalog #
60033AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, APC
Catalog #
60033AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, Biotin
Catalog #
100-0456, 100-0455, 60033BT.3
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, FITC
Catalog #
100-0454, 100-0453, 60033FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, PE
Catalog #
100-0452, 100-0451
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, PerCP-Cy5.5
Catalog #
100-0457
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119
Catalog #
60033
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, Alexa Fluor® 488
Catalog #
60033AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, APC
Catalog #
60033AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, Biotin
Catalog #
100-0456, 100-0455, 60033BT.3
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, FITC
Catalog #
100-0454, 100-0453, 60033FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, PE
Catalog #
100-0452, 100-0451
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, PerCP-Cy5.5
Catalog #
100-0457
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119
Catalog #
60033
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

更多信息

更多信息
Species Mouse
Clone TER-119
Gene Id 104231
Alternative Names Ly-76, TER-119
Isotype IgG2b, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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