产品号 #60033_C
Rat (WI) monoclonal IgG2b antibody against mouse TER119
概述
The TER-119 antibody reacts with murine TER119 (Ly-76), an ~52 kDa protein associated with glycophorin A on the surface of cells of the erythroid lineage in embryonic yolk sac, fetal and newborn liver, adult bone marrow, peripheral blood, and lymphoid organs. TER119 is an erythroid-specific marker expressed at all stages of differentation from early proerythroblasts to mature erythrocytes, but not by erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid). The TER-119 antibody is a component of the "lineage cocktail" used to detect, or deplete cells committed to hematopoietic lineages. In adult mice, TER119 is found on ~20 - 25% of bone marrow cells and ~2 - 3% of splenocytes.
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse CD4+ T Cell Isolation Kit (Catalog #19852).
Subtype
Primary Antibodies
Target Antigen
TER119
Alternative Names
Ly-76, TER-119
Reactive Species
Mouse
Conjugation
Biotin, FITC, PE, PerCP-Cyanine5.5
Host Species
Rat
Cell Type
Erythroid Cells, Red Blood Cells
Species
Mouse
Application
Cell Isolation, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
TER-119
Gene ID
104231
Isotype
IgG2b, kappa
Data Figures
Figure 1. Data for Unconjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, followed by goat antimouse IgG, FITC.
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with a rat IgG2b, kappa isotype control antibody followed by goat anti-mouse IgG, FITC.
Figure 2. Data for Alexa Fluor® 488-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, Alexa Fluor® 488 (filled histogram) or a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).
Figure 3. Data for Biotin-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER-119 Antibody, Clone TER-119, Biotin followed by streptavidin (SAV) PE (filled histogram) or a rat IgG2b, kappa isotype control antibody, Biotin followed by streptavidin (SAV) PE (solid line histogram).
Figure 4. Data for PE-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (solid line histogram).
Figure 5. Data for APC-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).
Figure 6. Data for FITC-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).
Figure 7. Data for PerCP-Cy55-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, APC Catalog #
60033AZ.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, Biotin Catalog #
100-0456, 100-0455, 60033BT.3 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, FITC Catalog #
100-0454, 100-0453, 60033FI Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, PE Catalog #
100-0452, 100-0451 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse TER119 Antibody, Clone TER-119 Catalog #
60033 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, APC Catalog #
60033AZ.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, Biotin Catalog #
100-0456, 100-0455, 60033BT.3 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, FITC Catalog #
100-0454, 100-0453, 60033FI Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, PE Catalog #
100-0452, 100-0451 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse TER119 Antibody, Clone TER-119 Catalog #
60033 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Dendritic Cell Research
Resources and Publications
Educational Materials (4)
Request Pricing
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
By submitting this form, you are providing your consent to STEMCELL Technologies Canada Inc. and its subsidiaries and affiliates (“STEMCELL”) to collect and use your information, and send you newsletters and emails in accordance with our
privacy policy. Please contact us with any questions that you may have. You can unsubscribe or change your email preferences at any time.
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
欢迎您关注STEMCELL Technologies的微信公众平台
扫描二维码或搜索微信号STEMCELLTech，即可关注我们的微信平台，第一时间接收丰富的技术资源和最新的活动信息。
如您有任何问题，欢迎发消息给STEMCELLTech微信公众平台，或与我们通过电话/邮件联系：400 885 9050 INFO.CN@STEMCELL.COM。