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抗小鼠Ly-6G抗体，克隆1A8

大鼠单克隆IgG2a抗体，抗小鼠Ly-6G
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产品号 #（选择产品）

产品号 #60031_C

大鼠单克隆IgG2a抗体，抗小鼠Ly-6G

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

1A8抗体识别Ly-6G，Ly-6G是一种21 - 25 kDa的GPI锚定蛋白，与结构上相关的Ly-6C蛋白一起组成粒细胞受体-1抗原（Gr-1）。Gr-1在单核细胞、中性粒细胞、巨噬细胞的亚群、浆细胞样树突状细胞和T细胞上表达。骨髓单核细胞在发育过程中短暂表达Gr-1，其表达水平与粒细胞分化和成熟密切相关。在外周，Gr-1主要存在于中性粒细胞上，是中性粒细胞的实用标志物。1A8抗体特异性地与Ly-6G结合，而另一种常用的抗体克隆RB6-8C5，既可以与Ly-6G又可以与Ly-6C结合。据报导，1A8抗体可用于检测外周血中表达Ly-6G的粒细胞，而RB6-8C5抗体也可以结合表达Ly-6C的淋巴细胞、单核细胞和树突状细胞。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™小鼠中性粒细胞富集试剂盒（产品号 #19762））分选的细胞纯度。

亚型
一抗
 
靶抗原
Ly-6G
 
别名
Gr-1，GR1，Ly6G
 
活性物种
小鼠
 
偶联
APC，Biotin 或 生物素，FITC，Pacific Blue，PE，PerCP-Cyanine5.5，未偶联的
 
宿主物种
大鼠
 
细胞类型
粒细胞及其亚群，髓系细胞
 
种属
小鼠
 
应用
流式细胞术，功能学筛选，免疫细胞化学，免疫沉淀
 
研究领域
免疫
 
克隆
1A8
 
基因编号
546644
 
同种型
IgG2a，kappa
 

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Data Figures

Data for FITC-Conjugated

Figure 1. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, FITC (filled histogram) or a rat IgG2a, kappa FITC isotype control antibody (solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, PE (filled histogram) or a rat IgG2a, kappa PE isotype control antibody (solid line histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram). (B) Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse uterine tissue labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, followed by anti-mouse, HRP. DAB substrate was used for visualization.

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) (solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 6. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Figure 7. Data for PB-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, Pacific Blue™ (filled histogram) or a rat IgG2a, kappa Pacific Blue™ isotype control antibody (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, APC
Catalog #
60031AZ, 60031AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, Biotin
Catalog #
60031BT.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, FITC
Catalog #
60031FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, Pacific Blue™
Catalog #
60031PB, 60031PB.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, PE
Catalog #
60031PE, 60031PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, PerCP-Cy5.5
Catalog #
60031PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8
Catalog #
60031
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, APC
Catalog #
60031AZ, 60031AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, Biotin
Catalog #
60031BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, FITC
Catalog #
60031FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, Pacific Blue™
Catalog #
60031PB, 60031PB.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, PE
Catalog #
60031PE, 60031PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, PerCP-Cy5.5
Catalog #
60031PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8
Catalog #
60031
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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更多信息
Species Mouse
Clone 1A8
Gene Id 546644
Alternative Names Gr-1, GR1, Ly6G
Isotype IgG2a, kappa
Legal Statement: Pacific Blue™ is a trademark of Life Technologies Corporation. Antibodies conjugated to Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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