产品号 #60002_C
抗小鼠CD11c的仓鼠（亚美尼亚）单克隆IgG抗体
总览
N418抗体可靶向CD11c（αX整合素）。CD11c是一种150 kDa的1型跨膜糖蛋白，其通过与CD18（β2整合素）非共价结合形成异二聚体细胞表面黏附受体。CD11c/CD18受体通过结合iC3b、纤维蛋白原和CD54等配体，参与多种免疫应答过程，包括细胞迁移、刺激单核细胞和巨噬细胞产生细胞因子、T细胞扩增、白细胞募集和吞噬作用。在小鼠中，CD11c在树突状细胞、巨噬细胞、单核细胞、粒细胞、NK细胞和部分T细胞上表达。
N418抗体克隆已通过验证，适用于EasySep™试剂盒（包括EasySep™小鼠CD11c正选试剂盒II，产品号 #18780）分离的细胞纯度检测。
亚型
一抗
靶抗原
CD11c
别名
alphaX整合素，CR4，整合素alphaX链，p150
活性物种
小鼠
偶联
Biotin 或 生物素，FITC，PE，未偶联的
宿主物种
Hamster
细胞类型
树突状细胞（DCs）
种属
小鼠
应用
CyTOF，流式细胞术，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀
研究领域
免疫
克隆
N418
基因编号
16411
同种型
IgG
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (3)
Publications (1)
Simple suspension culture system of human iPS cells maintaining their pluripotency for cardiac cell sheet engineering. Journal of Tissue Engineering and Regenerative Medicine 2015 DEC
Abstract
In this study, a simple three-dimensional (3D) suspension culture method for the expansion and cardiac differentiation of human induced pluripotent stem cells (hiPSCs) is reported. The culture methods were easily adapted from two-dimensional (2D) to 3D culture without any additional manipulations. When hiPSCs were directly applied to 3D culture from 2D in a single-cell suspension, only a few aggregated cells were observed. However, after 3 days, culture of the small hiPSC aggregates in a spinner flask at the optimal agitation rate created aggregates which were capable of cell passages from the single-cell suspension. Cell numbers increased to approximately 10-fold after 12 days of culture. The undifferentiated state of expanded hiPSCs was confirmed by flow cytometry, immunocytochemistry and quantitative RT-PCR, and the hiPSCs differentiated into three germ layers. When the hiPSCs were subsequently cultured in a flask using cardiac differentiation medium, expression of cardiac cell-specific genes and beating cardiomyocytes were observed. Furthermore, the culture of hiPSCs on Matrigel-coated dishes with serum-free medium containing activin A, BMP4 and FGF-2 enabled it to generate robust spontaneous beating cardiomyocytes and these cells expressed several cardiac cell-related genes, including HCN4, MLC-2a and MLC-2v. This suggests that the expanded hiPSCs might maintain the potential to differentiate into several types of cardiomyocytes, including pacemakers. Moreover, when cardiac cell sheets were fabricated using differentiated cardiomyocytes, they beat spontaneously and synchronously, indicating electrically communicative tissue. This simple culture system might enable the generation of sufficient amounts of beating cardiomyocytes for use in cardiac regenerative medicine and tissue engineering.
更多信息
| Species | Mouse |
|---|---|
| Clone | N418 |
| Gene Id | 16411 |
| Alternative Names | alphaX integrin, CR4, integrin alphaX chain, p150 |
| Isotype | IgG |
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