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抗小鼠CD19抗体，克隆ID3

大鼠单克隆IgG2a抗体，抗小鼠CD19
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产品号 #60112_C

大鼠单克隆IgG2a抗体，抗小鼠CD19

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

1D3抗体识别小鼠CD19，CD19是一种~95 kDa的1型跨膜糖蛋白，在从早期pre-B细胞到浆细胞的所有发育阶段的B细胞表面表达，在终末分化的浆细胞中表达下调但仍然存在。CD19也存在于滤泡树突状细胞中。CD19通过与CD21和CD81相结合，作为B细胞受体的共受体，参与B细胞的活化和分化。CD19的激活伴随着细胞质结构域的磷酸化，从而促进与激酶的结合并诱导细胞内信号级联反应。CD19的突变可导致严重免疫缺陷综合征。交叉竞争分析发现抗体克隆1D3与克隆6D5识别相同表位。

亚型
一抗
 
靶抗原
CD19
 
别名
B4
 
活性物种
小鼠
 
偶联
APC，Biotin 或 生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的
 
宿主物种
大鼠
 
细胞类型
B 细胞
 
种属
小鼠
 
应用
流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀
 
研究领域
免疫
 
克隆
1D3
 
基因编号
12478
 
同种型
IgG2a，kappa
 

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Data Figures

Data for unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or a rat IgG2a, kappa isotype control antibody followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, APC (filled histogram) or a rat IgG2a, kappa isotype control antibody, APC (solid line histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, Biotin, followed by streptavidin (SAV) APC (filled histogram), or a biotinylated rat IgG2a, kappa isotype control antibody followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, FITC (filled histogram) or a rat IgG2a, kappa isotype control antibody, FITC (solid line histogram).

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, PE (filled histogram) or a rat IgG2a, kappa isotype control antibody, PE (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 6. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, APC
Catalog #
60112AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, Biotin
Catalog #
60112BT.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, FITC
Catalog #
60112FI.1, 60112FI.2, 60112FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, PE
Catalog #
60112PE, 60112PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, PerCP-Cy5.5
Catalog #
60112PS, 60112PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3
Catalog #
60112
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, APC
Catalog #
60112AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, Biotin
Catalog #
60112BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, FITC
Catalog #
60112FI.1, 60112FI.2, 60112FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, PE
Catalog #
60112PE, 60112PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, PerCP-Cy5.5
Catalog #
60112PS, 60112PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3
Catalog #
60112
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

更多信息

更多信息
Species Mouse
Clone 1D3
Gene Id 12478
Alternative Names B4
Isotype IgG2a, kappa
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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