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EasySep™人Pan-ILC富集试剂盒

免疫磁珠负选细胞富集试剂盒
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产品号 #（选择产品）

产品号 #17975_C

免疫磁珠负选细胞富集试剂盒

产品优势

  • 操作简单、快捷，且无需分离柱
  • 获得的活细胞无标记
  • 利于快速流式分选ILC

产品组分包括

  • EasySep™ 人Pan-ILC富集试剂盒（产品号 #17975）
    • EasySep™人Pan-ILC2富集抗体混合物，0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，1 mL
  • RoboSep™ 人Pan-ILC富集试剂盒（产品号 #17975 RF）
    • EasySep™人Pan-ILC2富集抗体混合物，0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，1 mL
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号 #20125）
main product photo
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

EasySep™人Pan-ILC富集试剂盒通过免疫磁珠负选，从白细胞单采术样本中富集Group1、2和3 先天淋巴细胞（ILC1、2和3）。非目的细胞通过与抗非ILCs的抗体四聚体复合物及葡聚糖包被的磁珠（RapidSpheres™）结合而被去除。通过EasySep™磁极分离被标记的细胞，无需使用分离柱。富集的ILCs可被倾倒或吸取至一个新的试管中，非目标细胞仍保留在原管中。分选的细胞可被立即用于下游应用。

磁体兼容性
• “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
先天性淋巴细胞
 
种属
人
 
样本来源
Leukapheresis，PBMC
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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Data Figures

Typical EasySep™ Human Pan-ILC Enrichment Profile

Figure 1. Typical EasySep™ Human Pan-ILC Enrichment Profile

Starting with fresh leukapheresis samples, the total ILC content (Lin-CD45+CD127+) of the enriched fraction typically ranges from 17 - 85%. In the above example, the percentages of ILCs in the start and final enriched fractions are 0.09% and 45% (or 0.1% and 54% of CD45+ cells), respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Pan-ILC Enrichment Kit
Catalog #
17975
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Pan-ILC Enrichment Kit
Catalog #
17975RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Pan-ILC Enrichment Kit
Catalog #
17975
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Pan-ILC Enrichment Kit
Catalog #
17975
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Pan-ILC Enrichment Kit
Catalog #
17975RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Pan-ILC Enrichment Kit
Catalog #
17975RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human Pan-ILC Enrichment Kit
Catalog #
17975RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (12)

Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Cell Enrichment Prior to Cell Sorting
Brochure
Cell Enrichment Prior to Cell Sorting
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Innate Lymphoid Cells
Wallchart
Innate Lymphoid Cells
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Pipette Off Samples During Cell Isolation Using the EasyEights™ EasySep™ Cell Separation Magnet
1:16
Video
How to Pipette Off Samples During Cell Isolation Using the EasyEights™ EasySep™ Cell Separation ...
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
1:23
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
0:57
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
Pre-enrichment of Human ILCs Improves Sorting Efficiency While Maintaining Functionality
Scientific Poster
Pre-enrichment of Human ILCs Improves Sorting Efficiency While Maintaining Functionality
Efficient Enrichment of Functional ILC Subsets from Human PBMCs by Immunomagnetic Selection
Scientific Poster
Efficient Enrichment of Functional ILC Subsets from Human PBMCs by Immunomagnetic Selection

Publications (1)

Endothelin-A Receptor Antagonist Alleviates Allergic Airway Inflammation via the Inhibition of ILC2 Function. X. Zhang et al. Frontiers in immunology 2022

Abstract

Allergic airway inflammation is a universal airway disease that is driven by hyperresponsiveness to inhaled allergens. Group 2 innate lymphoid cells (ILC2s) produce copious amounts of type 2 cytokines, which lead to allergic airway inflammation. Here, we discovered that both peripheral blood of human and mouse lung ILC2s express the endothelin-A receptor (ETAR), and the expression level of ETAR was dramatically induced upon interleukin-33 (IL-33) treatment. Subsequently, both preventive and therapeutic effects of BQ123, an ETAR antagonist, on allergic airway inflammation were observed, which were associated with decreased proliferation and type 2 cytokine productions by ILC2s. Furthermore, ILC2s from BQ123 treatment were found to be functionally impaired in response to an interleukin IL-33 challenged. And BQ123 treatment also affected the phosphorylation level of the extracellular signal-regulated kinase (ERK), as well as the level of GATA binding protein 3 (GATA3) in activated ILC2s. Interestingly, after BQ123 treatment, both mouse and human ILC2s in vitro exhibited decreased function and downregulation of ERK signaling and GATA3 stability. These observations imply that ETAR is an important regulator of ILC2 function and may be involved in ILC2-driven pulmonary inflammation. Therefore, blocking ETAR may be a promising therapeutic strategy for allergic airway inflammation.
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更多信息

更多信息
Species Human
Magnet Compatibility • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
Sample Source Leukapheresis, PBMC
Selection Method Negative
标记抗体
  1. 抗人CD123（IL-3Rα）抗体，克隆 6H6
    抗人CD123（IL-3Rα）抗体，克隆 6H6

    抗人、恒河猴、白眉猴CD123 （IL-3Rα）的小鼠单克隆IgG1抗体

  2. 抗人CD16抗体，克隆号3G8
    抗人CD16抗体，克隆号3G8

    小鼠抗人、恒河猴、食蟹猴CD16单克隆IgG1抗体

  3. 抗人CD3抗体，克隆UCHT1
    抗人CD3抗体，克隆UCHT1

    小鼠（BALB/c）单克隆IgG1抗体，抗人、黑猩猩CD3

  4. 抗人CD45抗体，克隆HI30
    抗人CD45抗体，克隆HI30

    小鼠单克隆IgG1抗体，抗人、黑猩猩CD45

  5. 抗人CD14抗体，克隆M5E2
    抗人CD14抗体，克隆M5E2

    抗人、恒河猴、食蟹猴CD14的小鼠单克隆IgG2a抗体

  6. 抗人CD19抗体，克隆HIB19
    抗人CD19抗体，克隆HIB19

    抗人、黑猩猩CD19的小鼠单克隆IgG1抗体

  7. 抗人CD34抗体，克隆581
    抗人CD34抗体，克隆581

    小鼠单克隆IgG1抗体，抗人CD34

  8. 抗人CD117（c-Kit）抗体，克隆104D2
    抗人CD117（c-Kit）抗体，克隆104D2

    抗人、食蟹猴、牛CD117（c-Kit）的小鼠单克隆IgG1抗体

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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ISO 13485 和 ISO 9001 质量管理体系认证

ISO 14001 环境管理体系认证

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