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抗人CD117（c-Kit）抗体，克隆104D2

抗人、食蟹猴、牛CD117（c-Kit）的小鼠单克隆IgG1抗体
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产品号 #60087_C

抗人、食蟹猴、牛CD117（c-Kit）的小鼠单克隆IgG1抗体

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总览
实验数据
产品说明书及文档
应用领域
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总览

104D2抗体与CD117（c-Kit）反应。CD117是c-Kit配体（干细胞因子/钢因子）的1型跨膜受体，分子量约为145 kDa，广泛表达于骨髓造血干细胞，包括多能祖细胞、红系祖细胞、B淋巴细胞和T淋巴细胞前体细胞，以及肥大细胞和黑素细胞。CD117在约1 - 4%的骨髓细胞上表达，其中大多数（约50 - 70%）共表达CD34，包括祖细胞及其前体。CD117属于酪氨酸激酶受体家族，具有5个免疫球蛋白样C2型结构域和1个胞质蛋白激酶结构域。c-Kit配体与CD117结合可诱导二聚化和自磷酸化，从而激活几种对造血干细胞增殖和分化至关重要的细胞内信号通路。CD117信号传导通过磷酸酶和受体的快速内吞和降解来调控。104D2抗体的结合不会阻断c-Kit配体或抗体克隆SR1的结合。

亚型
一抗
 
靶抗原
CD117 (c-Kit)
 
别名
c-KIT，cKIT，干细胞因子受体（SCFR）
 
活性物种
Cow，Cynomolgus，人
 
偶联
APC，Biotin 或 生物素，PE，未偶联的
 
宿主物种
小鼠
 
细胞类型
内胚层，PSC衍生，造血干/祖细胞
 
种属
人，非人灵长类，其它细胞系
 
应用
CyTOF，流式细胞术，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀
 
研究领域
干细胞生物学
 
克隆
104D2
 
基因编号
3815
 
同种型
IgG1，kappa
 

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Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human induced pluripotent stem (iPS) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram). (C) Flow cytometry analysis of human embryonic stem (ES) cells following differentiation to definitive endoderm using the STEMdiff™ Definitive Endoderm Kit. Cells were labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC and Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, PE (Catalog #60089PE). The emergence of definitive endoderm can be monitored by co-expression of CD117 and CD184. (D) Flow cytometry analysis of human ES cells following differentiation to definitive endoderm using the STEMdiff™ Definitive Endoderm Kit. Cells were labeled with a mouse IgG1, kappa APC isotype control antibody and Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, Biotin, followed by streptavidin (SAV) APC (filled histogram), or a mouse IgG1, kappa biotin isotype control antibody, followed by SAV APC (solid line histogram).

Data for PE-Conjugated

Figure 4. Data for PE-Conjugated

Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, Biotin
Catalog #
60087BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC
Catalog #
60087AZ, 60087AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, PE
Catalog #
60087PE, 60087PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD117 (c-Kit) Antibody, Clone 104D2
Catalog #
60087, 60087.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, Biotin
Catalog #
60087BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC
Catalog #
60087AZ, 60087AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, PE
Catalog #
60087PE, 60087PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD117 (c-Kit) Antibody, Clone 104D2
Catalog #
60087, 60087.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (1)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research

更多信息

更多信息
Species Human, Non-Human Primate, Other
Clone 104D2
Gene Id 3815
Alternative Names c-KIT, cKIT, stem cell factor receptor (SCFR)
Isotype IgG1, kappa
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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