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抗人CD16抗体，克隆号3G8

小鼠抗人、恒河猴、食蟹猴CD16单克隆IgG1抗体
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产品号 #60041_C

小鼠抗人、恒河猴、食蟹猴CD16单克隆IgG1抗体

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

3G8抗体与CD16 （FcγRIII或低亲和力IgG受体III）反应，CD16是一种属于Ig超家族的1型跨膜糖蛋白。CD16以两种不同的亚型：CD16a，一种分子量为50 - 65 kDa的形式表达在NK细胞、活化的单核细胞、巨噬细胞和胎盘滋养细胞上；CD16b，一种~48 kDa的糖基磷脂酰肌醇（GPI）锚定形式表达在中性粒细胞、嗜碱性粒细胞和嗜酸性粒细胞上，发现至少有两种多态性变体，称为NA1和NA2。CD16与单体、聚合或复合形式IgG的Fc区结合较弱，特别是IgG1和IgG3同型。IgG与CD16亚型结合可诱导信号通路，调节多种类型的反应，包括抗体依赖性细胞介导的细胞毒性（ADCC）、吞噬、细胞因子释放和增殖。在基于抗体的检测和细胞分离实验中，CD16/IgG相互作用可导致非特异性标记，3G8抗体可作为阻断抗体减少非特异性结合。

亚型
一抗
 
靶抗原
CD16
 
别名
CD16A，CD16B，Fc-gamma RIII; FCG3; FCGR3; FCGRIII; FcγRIII; FCR-10，FcRIII，IGFR3，IMD20
 
活性物种
Baboon，Capuchin Monkey，Chimpanzee，Common Marmoset，Cotton-Topped Tamarin，Cynomolgus，人，Macaque，Rhesus，Sooty Mangabey，Squirrel Monkey
 
偶联
Alexa Fluor 488，APC，Biotin 或 生物素，FITC，PE，未偶联的
 
宿主物种
小鼠
 
细胞类型
粒细胞及其亚群，单核细胞
 
种属
人，非人灵长类
 
应用
细胞分选，CyTOF，删除，ELISAs，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，中和及阻断
 
研究领域
免疫
 
克隆
3G8
 
基因编号
2214
 
同种型
IgG1，kappa
 

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Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, Alexa Fluor® 488 (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (Catalog #60070AD) (solid line histogram).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, APC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60070AZ) (solid line histogram).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin (Catalog #60070BT), followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI) (solid line histogram).

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, PE (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE) (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD16 Antibody, Clone 3G8, Alexa Fluor® 488
Catalog #
60041AD.1, 60041AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD16 Antibody, Clone 3G8, APC
Catalog #
60041AZ.1, 60041AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD16 Antibody, Clone 3G8
Catalog #
60041.1, 60041
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD16 Antibody, Clone 3G8, PE
Catalog #
60041PE.1, 60041PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD16 Antibody, Clone 3G8, FITC
Catalog #
60041FI, 60041FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD16 Antibody, Clone 3G8, Biotin
Catalog #
60041BT.1, 60041BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD16 Antibody, Clone 3G8, Alexa Fluor® 488
Catalog #
60041AD.1, 60041AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD16 Antibody, Clone 3G8, APC
Catalog #
60041AZ.1, 60041AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD16 Antibody, Clone 3G8
Catalog #
60041.1, 60041
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD16 Antibody, Clone 3G8, PE
Catalog #
60041PE.1, 60041PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD16 Antibody, Clone 3G8, FITC
Catalog #
60041FI, 60041FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD16 Antibody, Clone 3G8, Biotin
Catalog #
60041BT.1, 60041BT
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human, Non-Human Primate
Clone 3G8
Gene Id 2214
Alternative Names CD16A, CD16B, Fc-gamma RIII; FCG3; FCGR3; FCGRIII; FcγRIII; FCR-10, FcRIII, IGFR3, IMD20
Isotype IgG1, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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