产品号 #60041_C
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD16
概述
The 3G8 antibody reacts with CD16 (FcγRIII or low affinity IgG receptor III), a type 1 transmembrane glycoprotein belonging to the Ig superfamily. CD16 exists in two distinct isoforms: CD16a, a 50 - 65 kDa form expressed on NK cells, activated monocytes, macrophages, and placental trophoblasts, and CD16b, an ~48 kDa glycosylphosphatidylinositol (GPI)-anchored form expressed on neutrophils, basophils and eosinophils and found as at least two polymorphic variants, termed NA1 and NA2. CD16 binds weakly to the Fc region of monomeric, aggregated or complexed IgG, particularly the IgG1 and IgG3 isotypes. Binding of IgG to either CD16 isoform induces signaling pathways that modulate several types of responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release and proliferation. CD16/IgG interactions can result in non-specific labeling in antibody-based detection and cell separation experiments and the 3G8 antibody may be employed as a blocking antibody to reduce non-specific binding.
Subtype
Primary Antibodies
Target Antigen
CD16
Alternative Names
CD16A, CD16B, Fc-gamma RIII; FCG3; FCGR3; FCGRIII; FcγRIII; FCR-10, FcRIII, IGFR3, IMD20
Reactive Species
Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-Topped Tamarin, Cynomolgus, Human, Macaque, Rhesus, Sooty Mangabey, Squirrel Monkey
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
Granulocytes and Subsets, Monocytes
Species
Human, Non-Human Primate
Application
Cell Isolation, CyTOF, Depletion, ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Neutralization and Blocking
Area of Interest
Immunology
Clone
3G8
Gene ID
2214
Isotype
IgG1, kappa
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (4)
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
