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抗人CD3抗体，克隆UCHT1

小鼠（BALB/c）单克隆IgG1抗体，抗人、黑猩猩CD3
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产品号 #60011_C

小鼠（BALB/c）单克隆IgG1抗体，抗人、黑猩猩CD3

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应用领域
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总览

UCHT1抗体识别人T细胞受体(TCR)/CD3复合物的~ 20kda CD3ε亚基，该亚基在~95%的成熟T细胞和NKT细胞表面表达，在胸腺细胞上呈可变表达。大多数T细胞瘤也表达CD3。CD3复合物由CD3γ、δ、ε、η和ζ亚基组合而成，与TCR非共价结合，参与将抗原识别信号转导到T细胞的胞质中，并调节TCR的细胞表面表达。TCR介导的T细胞激活涉及CD3亚基的胞质尾部，CD3 亚基是结构相关的 1 型跨膜蛋白，属于免疫球蛋白超家族。CD3亚基的突变与多种免疫缺陷疾病有关，包括严重的联合免疫缺陷（SCID）。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™Direct人T细胞分选试剂盒（产品号 #19661），EasySep™人CD3正选试剂盒II（产品号 #17851；可能观察到部分阻断），EasySep™HLA全血T细胞富集试剂盒（产品号 #19951HLA）和EasySep™HLA全血CD2正选试剂盒（产品号 #18687HLA））分选的细胞纯度。

亚型
一抗
 
靶抗原
CD3
 
别名
CD3e，CD3epsilon，T3
 
活性物种
Chimpanzee，人
 
偶联
APC，FITC，PE
 
宿主物种
小鼠
 
细胞类型
T 细胞
 
种属
人，非人灵长类
 
应用
CyTOF，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
 
研究领域
免疫
 
克隆
UCHT1
 
基因编号
915
 
同种型
IgG1，kappa
 

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Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD3 Positive Selection Kit and labeled with Anti-Human CD3 Antibody, Clone UCHT1, APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (open histogram).
(C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood T Cell Enrichment Kit (Catalog #19951HLA). Histograms show labeling of HetaSep™-treated whole blood cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (open histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, Biotin followed by streptavidin (SAV) APC (filled histogram) or a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC (black line histogram).
(B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD2 Positive Selection Kit and labeled with Anti-Human CD3 Antibody, Clone UCHT1, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibodyfollowed by SAV APC is shown (open histogram).
(C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD3 Positive Selection Kit and labeled with Anti-Human CD3 Antibody, Clone UCHT1, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibodyfollowed by SAV APC is shown (open histogram).

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood T Cell Enrichment Kit and labeled with Anti-Human CD3 Antibody, Clone UCHT1, FITC. Histograms show labeling of HetaSep™-treated whole blood cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (open histogram).
(C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD3 Positive Selection Kit and labeled with Anti-Human CD3 Antibody, Clone UCHT1, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (open histogram).

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).

Data for Unconjugated

Figure 6. Data for Unconjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, followed by goat anti-mouse IgG, FITC (filled histogram). Labeling with a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC is shown (solid line histogram).
(B) Flow cytometry analysis of PBMCs prior to being processed with EasySep™ Human CD3 Positive Selection Kit (Catalog #18051), which uses Anti-Human CD3 Antibody, Clone UCHT1, to select CD3+ cells. The cells were labeled with Anti-Human CD4 Antibody, Clone OKT4, Alexa Fluor® 488 (Catalog #60016AD) and Anti-Human CD8 Antibody, Clone RPA-T8, PE (Catalog #60022PE).
(C) Flow cytometry analysis of PBMCs processed with EasySep™ Human CD3 Positive Selection Kit and labeled with Anti-Human CD4 Antibody, Clone OKT4, Alexa Fluor® 488 (Catalog #60016AD) and Anti-Human CD8 Antibody, Clone RPA-T8 (Catalog #60022PE).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, PerCP-Cy5.5 (filled histogram) or a mouse IgG1, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD3 Positive Selection Kit (Catalog #18051) and labeled with Anti-Human CD3 Antibody, Clone UCHT1, PerCP-Cy5.5. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa isotype control antibody, PerCP-Cy5.5 is shown in the bottom panel (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 8. Data for PerCP-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, PerCP (filled histogram) or a mouse IgG1, kappa isotype control antibody, PerCP (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD3 Antibody, Clone UCHT1, PE
Catalog #
100-0449, 60011PE.1, 60011PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD3 Antibody, Clone UCHT1, APC
Catalog #
60011AZ.1, 60011AZ, 100-0448
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD3 Antibody, Clone UCHT1, Biotin
Catalog #
60011BT, 60011BT.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD3 Antibody, Clone UCHT1, FITC
Catalog #
60011FI, 60011FI.1, 100-0447
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD3 Antibody, Clone UCHT1, Alexa Fluor® 488
Catalog #
60011AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD3 Antibody, Clone UCHT1
Catalog #
60011
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD3 Antibody, Clone UCHT1, PerCP
Catalog #
60011PR.1, 60011PR
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD3 Antibody, Clone UCHT1, PerCP-Cy5.5
Catalog #
60011PS.1, 60011PS
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD3 Antibody, Clone UCHT1, PE
Catalog #
100-0449, 60011PE.1, 60011PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD3 Antibody, Clone UCHT1, APC
Catalog #
60011AZ.1, 60011AZ, 100-0448
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD3 Antibody, Clone UCHT1, Biotin
Catalog #
60011BT, 60011BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD3 Antibody, Clone UCHT1, FITC
Catalog #
60011FI, 60011FI.1, 100-0447
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD3 Antibody, Clone UCHT1, Alexa Fluor® 488
Catalog #
60011AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD3 Antibody, Clone UCHT1
Catalog #
60011
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD3 Antibody, Clone UCHT1, PerCP
Catalog #
60011PR.1, 60011PR
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD3 Antibody, Clone UCHT1, PerCP-Cy5.5
Catalog #
60011PS.1, 60011PS
Lot #
All
Language
English

更多信息

更多信息
Species Human, Non-Human Primate
Clone UCHT1
Gene Id 915
Alternative Names CD3e, CD3epsilon, T3
Isotype IgG1, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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