切换导航
  • 比较产品
我的购物车

抗人CD34抗体，克隆581

小鼠单克隆IgG1抗体，抗人CD34
率先评论此产品
添加并比较

产品号 #（选择产品）

产品号 #60013_C

小鼠单克隆IgG1抗体，抗人CD34

main product photo
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

581抗体可识别大多数人造血干祖细胞（HSPCs）以及间充质干细胞、胚胎成纤维细胞、内皮细胞、神经元和某些肿瘤细胞系表面表达的1型跨膜糖蛋白CD34。CD34仅在造血过程中短暂表达，因此CD34+细胞在骨髓或脐带血中的频率较低（~1 - 5%），在外周血中的频率极低（~0.1 - 0.5%）。CD34是一种用于鉴定和分选具有细胞植入能力的造血干细胞的标志物。CD34被认为在早期造血过程中介导干细胞与骨髓细胞外基质或直接与基质细胞的附着，并通过结合配体L-和E-选择素参与淋巴细胞募集。CD34的表位基于对酶裂解的敏感性可分成不同的表位组，581抗体识别III类表位（对神经氨酸酶和O-糖蛋白酶具有抗性）。

该抗体克隆已被验证可用于评估EasySep™人CD34正选试剂盒（产品号 #18056）分选的细胞纯度，以及标记使用MesenCult™增殖试剂盒（人；产品号 #05411）培养的人间充质干细胞。

亚型
一抗
 
靶抗原
CD34
 
别名
Gp105-120，My10
 
活性物种
人
 
偶联
Alexa Fluor 488，APC，Biotin 或 生物素，FITC，PE，未偶联的
 
宿主物种
小鼠
 
细胞类型
造血干/祖细胞
 
种属
人
 
应用
细胞分选，CyTOF，流式细胞术，免疫细胞化学，免疫荧光，免疫组化
 
研究领域
干细胞生物学
 
克隆
581
 
基因编号
947
 
同种型
IgG1，kappa
 

查看更多 显示更少

Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD34 Antibody, Clone 581, Alexa Fluor® 488 and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of PBMCs labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (Catalog #60070AD)and Anti-Human CD45 Antibody, Clone HI30, APC. (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD34 Positive Selection Kit and labeled with Anti-Human CD34 Antibody, Clone 581, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 is shown (solid line histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD34 Antibody, Clone 581, APC and Anti-Human CD45 Antibody, Clone HI30, PE (Catalog #60018PE). (B) Flow cytometry analysis of human PBMCs labeled with a mouse IgG1, kappa APC isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, PE. (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD34 Positive Selection Kit and labeled with Anti-Human CD34 Antibody, Clone 581, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (solid line histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD34 Antibody, Clone 581, Biotin followed by streptavidin (SAV) APC and Anti-Human CD45 Antibody, Clone HI30, PE (Catalog #60018PE).
(B) Flow cytometry analysis of human PBMCs labeled with a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC and Anti-Human CD45 Antibody, Clone HI30, PE.

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD34 Antibody, Clone 581, FITC and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human PBMCs labeled with a mouse IgG1, kappa FITC isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, APC. (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD34 Positive Selection Kit and labeled with Anti-Human CD34 Antibody, Clone 581, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (solid line histogram).

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD34 Antibody, Clone 581, PE and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of PBMCs labeled with a mouse IgG1, kappa PE isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, APC. (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD34 Positive Selection Kit and labeled with Anti-Human CD34 Antibody, Clone 581, PE. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).

Data for Unconjugated

Figure 6. Data for Unconjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD34 Antibody, Clone 581, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human PBMCs labeled with a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC and Anti-Human CD45 Antibody, Clone HI30, APC. (C) Flow cytometry analysis of human KG1a myeloid cells labeled with Anti-Human CD34 Antibody, Clone 581, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (filled histogram), or a mouse IgG1, kappa isotype control antibody followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD34 Antibody, Clone 581, Alexa Fluor® 488
Catalog #
60013AD, 60013AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD34 Antibody, Clone 581, FITC
Catalog #
60013FI, 60013FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD34 Antibody, Clone 581, Biotin
Catalog #
60013BT, 60013BT.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD34 Antibody, Clone 581
Catalog #
60013
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD34 Antibody, Clone 581, APC
Catalog #
60013AZ.1, 60013AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD34 Antibody, Clone 581, PE
Catalog #
60013PE.1, 60013PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD34 Antibody, Clone 581, Alexa Fluor® 488
Catalog #
60013AD, 60013AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD34 Antibody, Clone 581, FITC
Catalog #
60013FI, 60013FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD34 Antibody, Clone 581, Biotin
Catalog #
60013BT, 60013BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD34 Antibody, Clone 581
Catalog #
60013
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD34 Antibody, Clone 581, APC
Catalog #
60013AZ.1, 60013AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD34 Antibody, Clone 581, PE
Catalog #
60013PE.1, 60013PE
Lot #
All
Language
English

更多信息

更多信息
Species Human
Clone 581
Gene Id 947
Alternative Names Gp105-120, My10
Isotype IgG1, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
版权 © 2025 STEMCELL Technologies 技术有限公司。保留所有权利。