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EasySep™人浆细胞样DC分选试剂盒

免疫磁珠负选未标记的人浆细胞样DC细胞
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产品号 #（选择产品）

产品号 #17977_C

免疫磁珠负选未标记的人浆细胞样DC细胞

产品优势

  • 操作简单、快捷，且无需分离柱
  • 纯度高达97%
  • 获得的活细胞无标记

产品组分包括

  • EasySep™人浆细胞样DC分选试剂盒（产品号 #17977）
    • EasySep™人浆细胞样DC分选抗体混合物组分 A，2 x 1 mL
    • EasySep™人浆细胞样DC分选抗体混合物组分B，2 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50103 磁珠，4 x 1 mL
    • 抗人CD32 (Fc gamma RII) 阻断剂， 2 x 0.8 mL
main product photo
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用EasySep™人浆细胞样DC细胞分选试剂盒，可轻松高效地从新鲜人外周血单个核细胞（PBMCs）或白细胞单采术样本中，通过免疫磁珠负选获得高纯度人浆细胞样树突状细胞（pDCs）。EasySep™技术结合单克隆抗体的特异性和无柱磁分选系统的简便性，已在发表的研究中广泛应用超过20年。

在该EasySep™负选流程中，非目的细胞被抗体复合物与磁珠标记。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着简单地将目的细胞倾倒或吸取至一个新的试管中。经磁珠分选后，目的pDCs可立即用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。

该产品可替代EasySep™人浆细胞样DC富集试剂盒 (产品号 #19062) 以进行更快地细胞分选，并适配于所有的EasySep™磁极。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化。或直接选用即用型、符合伦理来源的用EasySep™人浆细胞样DC分选试剂盒分选的冻存原代人外周血浆细胞样树突状细胞。探索更多为您实验流程优化的其它产品，包括培养基、补充剂、抗体等。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • Easy 50 EasySep™ Magnet (Catalog #18002) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
树突状细胞（DCs）
 
种属
人
 
样本来源
Leukapheresis，PBMC
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫，感染性疾病(传染病)
 

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Data Figures

FACS Profile Results with EasySep™ Human Plasmacytoid DC Isolation Kit

Figure 1. Typical EasySep™ Human Plasmacytoid DC Isolation Profile

Starting with leukapheresis samples, the pDC content (Lin-HLA-DR+CD123+) of the isolated fraction is typically 90 ± 5.3% (mean ± SD) using the silver “Big Easy” EasySep™ Magnet. In the above example, the purities of the start and final isolated fractions are 0.2% and 89.8%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Plasmacytoid DC Isolation Kit
Catalog #
17977RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Human Plasmacytoid DC Isolation Kit
Catalog #
17977
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Plasmacytoid DC Isolation Kit
Catalog #
17977RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Plasmacytoid DC Isolation Kit
Catalog #
17977RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human Plasmacytoid DC Isolation Kit
Catalog #
17977RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Human Plasmacytoid DC Isolation Kit
Catalog #
17977RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
RoboSep™ Human Plasmacytoid DC Isolation Kit
Catalog #
17977RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Plasmacytoid DC Isolation Kit
Catalog #
17977
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Plasmacytoid DC Isolation Kit
Catalog #
17977
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human Plasmacytoid DC Isolation Kit
Catalog #
17977
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Human Plasmacytoid DC Isolation Kit
Catalog #
17977
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (5)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Cell Enrichment Prior to Cell Sorting
Brochure
Cell Enrichment Prior to Cell Sorting
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Antigen Processing and Presentation
Wallchart
Antigen Processing and Presentation
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation

Publications (2)

HMGB1 Is Involved in IFN-α Production and TRAIL Expression by HIV-1-Exposed Plasmacytoid Dendritic Cells: Impact of the Crosstalk with NK Cells. Saï et al. PLoS pathogens 2016 FEB

Abstract

Plasmacytoid dendritic cells (pDCs) are innate sensors of viral infections and important mediators of antiviral innate immunity through their ability to produce large amounts of IFN-α. Moreover, Toll-like receptor 7 (TLR7) and 9 (TLR9) ligands, such as HIV and CpG respectively, turn pDCs into TRAIL-expressing killer pDCs able to lyse HIV-infected CD4+ T cells. NK cells can regulate antiviral immunity by modulating pDC functions, and pDC production of IFN-α as well as cell-cell contact is required to promote NK cell functions. Impaired pDC-NK cell crosstalk was reported in the setting of HIV-1 infection, but the impact of HIV-1 on TRAIL expression and innate antiviral immunity during this crosstalk is unknown. Here, we report that low concentrations of CCR5-tropic HIV-1Ba-L promote the release of pro-inflammatory cytokines such as IFN-α, TNF-α, IFN-γ and IL-12, and CCR5-interacting chemokines (MIP-1α and MIP-1β) in NK-pDCs co-cultures. At high HIV-1BaL concentrations, the addition of NK cells did not promote the release of these mediators, suggesting that once efficiently triggered by the virus, pDCs could not integrate new activating signals delivered by NK cells. However, high HIV-1BaL concentrations were required to trigger IFN-α-mediated TRAIL expression at the surface of both pDCs and NK cells during their crosstalk. Interestingly, we identified the alarmin HMGB1, released at pDC-NK cell synapse, as an essential trigger for the secretion of IFN-α and IFN-related soluble mediators during the interplay of HIV-1 exposed pDCs with NK cells. Moreover, HMGB1 was found crucial for mTRAIL translocation to the plasma membrane of both pDCs and NK cells during their crosstalk following pDC exposure to HIV-1. Data from serum analyses of circulating HMGB1, HMGB1-specific antibodies, sTRAIL and IP-10 in a cohort of 67 HIV-1+ patients argue for the in vivo relevance of these observations. Altogether, these findings identify HMGB1 as a trigger for IFN-α-mediated TRAIL expression at the surface of pDCs and NK cells, and they suggest a novel mechanism of innate control of HIV-1 infection.
View publication
Plasmacytoid dendritic cells reduce HIV production in elite controllers. Machmach K et al. Journal of virology 2012 APR

Abstract

HIV elite controllers (EC) are a rare group of HIV-infected patients who are able to maintain undetectable viral loads during a long period of time in the absence of antiretroviral treatment. Adaptive immunity and host genetic factors, although implicated, do not entirely explain this phenomenon. On the other hand, plasmacytoid dendritic cells (pDCs) are the principal type I interferon (IFN) producers in response to viral infection, and it is unknown whether pDCs are involved in the control of HIV infection in EC. In our study, we analyzed peripheral pDC levels and IFN-α production by peripheral blood mononuclear cells (PBMCs) in EC compared to other groups of HIV-infected patients, the ability of pDCs to reduce HIV production in vitro, and the mechanisms potentially involved. We showed preserved pDC counts and IFN-α production in EC. We also observed a higher capacity of pDCs from EC to reduce HIV production and to induce T cell apoptosis, whereas pDCs from viremic patients barely responded without previous Toll-like receptor 9 (TLR-9) stimulus. The preserved functionality of pDCs from EC to reduce viral production may be one of the mechanisms involved in the control of HIV viremia in these subjects. These results demonstrate the importance of innate immunity in HIV pathogenesis, and an understanding of pDC mechanisms would be helpful for the design of new therapies.
View publication
View All Publications

更多信息

更多信息
Species Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • Easy 50 EasySep™ Magnet (Catalog #18002) • RoboSep™-S (Catalog #21000)
Sample Source Leukapheresis, PBMC
Selection Method Negative
标记抗体
  1. 抗人CD14抗体，克隆M5E2
    抗人CD14抗体，克隆M5E2

    抗人、恒河猴、食蟹猴CD14的小鼠单克隆IgG2a抗体

  2. 抗人CD20抗体，克隆2H7
    抗人CD20抗体，克隆2H7

    小鼠单克隆IgG2b抗体，抗人、恒河猴、食蟹猴CD20

  3. 抗人CD45抗体，克隆HI30
    抗人CD45抗体，克隆HI30

    小鼠单克隆IgG1抗体，抗人、黑猩猩CD45

  4. 抗人CD16抗体，克隆号3G8
    抗人CD16抗体，克隆号3G8

    小鼠抗人、恒河猴、食蟹猴CD16单克隆IgG1抗体

  5. 抗人CD3抗体，克隆SK7
    抗人CD3抗体，克隆SK7

    抗人、黑猩猩CD3的小鼠单克隆抗体

  6. 抗人HLA-DR抗体，克隆LN3
    抗人HLA-DR抗体，克隆LN3

    抗人、恒河猴HLA-DR的小鼠单克隆IgG2b抗体

  7. 抗人CD123（IL-3Rα）抗体，克隆 6H6
    抗人CD123（IL-3Rα）抗体，克隆 6H6

    抗人、恒河猴、白眉猴CD123 （IL-3Rα）的小鼠单克隆IgG1抗体

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