产品号 #76005_C
增强的绿色荧光蛋白（eGFP）标记的Cas9核酸酶在CRISPR-Cas9基因组编辑中产生双链断裂
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
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ArciTect™ sgRNACustom-designed single guide RNA for CRISPR-Cas9 genome editing
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ArciTect™ crRNACustom-designed CRISPR RNA for guide RNA generation in CRISPR-Cas9 genome editing
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ArciTect™ tracrRNA KitTrans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing
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Nuclease-Free WaterDNase- and RNase-free water for molecular biology applications
概述
architect™Cas9-eGFP核酸酶是一种融合蛋白，由增强型绿色荧光蛋白（eGFP）和来自化脓性链球菌的野生型Cas9重组蛋白组成。architect™Cas9-eGFP核酸酶含有一个c端连接的eGFP分子。architect™Cas9-eGFP核酸酶需要与一个引导rna结合。architect™sgRNA（目录#200-0013）或由architect™tracrRNA（目录#76016）和architect™crRNA（目录#76010）组成的双工，形成核糖核蛋白（RNP）复合物。这种RNP复合体在基因组的特定位置产生双链断裂。architect™Cas9-eGFP核酸酶还在n端包含一个核定位信号，确保RNP复合体易位到细胞核，从而提高基因组编辑的效率。由于RNP复合体在转染时功能完全，它允许在易位到细胞核后立即活动。RNP复合物在48小时内降解，为基因组编辑提供了足够的时间，同时减少了RNP复合物持续存在可能引起的脱靶效应。使用RNP系统还避免了生成稳定表达cas9的细胞系的繁琐过程，节省了时间并降低了由于诱导表达系统泄漏而导致脱靶效应的风险。化脓性链球菌Cas9使用原间隔器相邻基序（PAM）序列NGG（其中N可以是任何核苷酸）。如果没有目标序列下游的基因组PAM位点，该酶将无法切割
Cell Type
Pluripotent Stem Cells
Species
Human
Application
Genome Editing
Area of Interest
Stem Cell Biology
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (14)
更多信息
| Species | Human |
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