切换导航
  • 比较产品
我的购物车

ArciTect™ T7 核酸内切酶 I 试剂盒

用于评估CRISPR-Cas9基因组编辑效率
率先评论此产品
添加并比较

产品号 #（选择产品）

产品号 #76021_C

用于评估CRISPR-Cas9基因组编辑效率

产品组分包括

  • ArciTect™ T7 核酸内切酶 I 试剂盒，25 Reactions（产品号 #76021）
    • ArciTect™ T7 核酸内切酶 I，25 Reactions
    • ArciTect™ T7 核酸内切酶 I 缓冲液 (10X)，1 mL
  • ArciTect™ T7 核酸内切酶 I 试剂盒，125 Reactions（产品号 #76022）
    • ArciTect™ T7 核酸内切酶 I，125 Reactions
    • ArciTect™ T7 核酸内切酶 I 缓冲液 (10X)，1 mL
main product photo
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

ArciTect™ T7 核酸内切酶 I 是检测由 CRISPR-Cas9 产生的插入或缺失（INDELs）等基因组编辑的首选酶。ArciTect™ T7 核酸内切酶 I 试剂盒包含 ArciTect™ T7 核酸内切酶 I 和 ArciTect™ T7 核酸内切酶 I 缓冲液 (10X)，经测试和验证，可与 ArciTect™ CRISPR-Cas9 基因组编辑系统配合使用。ArciTect™ T7 核酸内切酶 I 能识别并切割错配 DNA、十字形 DNA 结构、Holliday 结构或连接区、异源双链 DNA，以及低效切割的缺口双链 DNA。由于酶切效率与特定位点产生的 INDEL 数量成正比，因此 ArciTect™ T7 核酸内切酶 I 试剂盒可用于快速、经济地评估基因编辑效率。

细胞类型
多能干细胞
 
种属
人
 
应用
基因组编辑
 
研究领域
干细胞生物学
 

查看更多 显示更少

Data Figures

Figure 1. INDEL Detection by T7 Endonuclease I Assay

Human embryonic stem (ES) and induced pluripotent stem (iPS) cells (A) and T cells (B) were edited using ArciTect™ Cas9 Nuclease (Catalog #76002) and ArciTect™ Human HPRT Positive Control Kit (Catalog #76013), and INDEL formation was assessed using ArciTect™ T7 Endonuclease I Kit. Following CRISPR-mediated editing at the HPRT locus, genomic DNA was isolated and a 1 kb region surrounding the target site was amplified by PCR using ArciTect™ Human HPRT Primer Mix (included with Catalog #76013). PCR products were purified, then denatured, re annealed, and cut with ArciTect™ T7 Endonuclease I. Samples were resolved on a 1% agarose gel, and band intensities were determined using a ChemiDoc™ MP Imaging System (Bio-Rad). Relative intensities of the full length (1083 base pairs [bp]) and T7 cleavage product bands (827 and 256 bp) were used to calculate the cleavage efficiency (%). Control: Uncut PCR product (no T7 added); Test, Donor 1, and Donor 2: T7-digested PCR product.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ArciTect™ T7 Endonuclease I Kit
Catalog #
76021, 76022
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
ArciTect™ T7 Endonuclease I Kit
Catalog #
76021, 76022
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
ArciTect™ T7 Endonuclease I Kit
Catalog #
76021, 76022
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

更多信息

更多信息
Species Human
Legal Statement: The purchase of the ArciTect™ products conveys to the purchaser the limited, non-transferable right to use, in accordance with applicable laws and regulations, such purchased products and any related material solely for research purposes only, not for any commercial purposes, with no warranty from the owner or assignee of the Patents, and with no liability to the owner or assignee of the Patents, or a director, trustee, officer, employee, agent, faculty, official investigator or student thereof. Distribution of ArciTect™ products is covered under at least US 8,697,359, US 8,771,945, US 8,795,965, US 8,865,406, US 8,871,445, US 8,889,356, US 8,889,418, US 8,895,308, US 8,906,616 and foreign equivalents (“Patents”). PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
版权 © 2025 STEMCELL Technologies 技术有限公司。保留所有权利。