产品号 #76001_C
Cas9 核酸酶，用于在 CRISPR-Cas9 基因组编辑中产生双链断裂
总览
ArciTect™ Cas9 核酸酶需要与向导RNA（例如 ArciTect™ sgRNA（目录号：200-0013）或由 ArciTect™ tracrRNA（目录号：76016）和 ArciTect™ crRNA（目录号：76010）组成的双链）结合，形成核糖核蛋白 (RNP) 复合物。该 RNP 复合物随后可在基因组中的特定位点产生双链断裂。ArciTect™ Cas9 核酸酶在 N 端含有核定位信号，确保 RNP 复合物转位至细胞核，从而提高基因组编辑效率。由于该 RNP 复合物在转染后即具备完全功能，它能在进入细胞核后立即发挥作用。RNP 复合物在约 48 小时内被降解，这为基因编辑提供了充足的时间，同时减少了因 RNP 持续存在而引起的脱靶效应。使用 RNP 系统还可以避免构建稳定表达 Cas9 的细胞系的繁琐过程，从而节省时间并降低因可诱导表达系统泄漏而导致脱靶效应的风险。化脓性链球菌的 Cas9 蛋白识别的原间隔区相邻基序（PAM）为 NGG（其中 N 表示任意核苷酸）。如果目标序列下游没有基因组PAM位点，该酶将无法切割。
细胞类型
多能干细胞
种属
人
应用
基因组编辑
研究领域
干细胞生物学
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (13)
更多信息
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