产品号 #76005_C
增强型绿色荧光蛋白 (eGFP) 标记的 Cas9 核酸酶，用于在 CRISPR-Cas9 基因组编辑中产生双链断裂
总览
ArciTect™ Cas9-eGFP 核酸酶是一种融合蛋白，由增强型绿色荧光蛋白 (eGFP) 和来自化脓性链球菌 (Streptococcus pyogenes) 的野生型 Cas9 重组蛋白组成。ArciTect™ Cas9-eGFP 核酸酶含有一个 C 端连接的 eGFP 分子。ArciTect™ Cas9-eGFP 核酸酶需要与向导 RNA（例如 ArciTect™ sgRNA，目录号 #200-0013，或由 ArciTect™ tracrRNA（目录号 #76016）和 ArciTect™ crRNA（目录号 #76010）组成的双链 RNA）结合，以形成核糖核蛋白（RNP）复合物。该 RNP 复合物能够在基因组中特定位点产生双链断裂。ArciTect™ Cas9-eGFP 核酸酶的 N 端还包含核定位信号，确保 RNP 复合物转位至细胞核，从而提高基因组编辑的效率。由于该 RNP 复合物在转染后即具备完全功能，它能在进入细胞核后立即发挥作用。RNP 复合物在约 48 小时内被降解，这为基因编辑提供了充足的时间，同时减少了因 RNP 持续存在而引起的脱靶效应。使用 RNP 系统还可以避免构建稳定表达 Cas9 的细胞系的繁琐过程，从而节省时间并降低因可诱导表达系统泄漏而导致脱靶效应的风险。化脓性链球菌的 Cas9 蛋白识别的原间隔区相邻基序（PAM）为 NGG（其中 N 表示任意核苷酸）。如果目标序列下游没有基因组PAM位点，该酶将无法切割。
细胞类型
多能干细胞
种属
人
应用
基因组编辑
研究领域
干细胞生物学
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
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Resources and Publications
Educational Materials (14)
更多信息
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Legal Statement: The purchase of the ArciTect™ products conveys to the purchaser the limited, non-transferable right to use, in accordance with all applicable laws and regulations, the ArciTect™ products and any related material solely for research purposes only, not for any clinical, human, agricultural, veterinary, livestock, or commercial purpose, with no warranty (express or implied) from the owners or assignees of the Patents or STEMCELL Technologies each of whom expressly disclaim any warranty regarding results obtained through the use of the ArciTect™ products, and without any exception the owners or assignees of the Patents or STEMCELL Technologies, or as applicable a director, trustee, officer, employee, agent, faculty, official investigator or student thereof, will not suffer or be exposed to any liability, damages, loss, or expense of any kind whatsoever arising from or related to the use of the ArciTect™ products by a purchaser of same. Distribution of ArciTect™ products by STEMCELL Technologies is covered under at least US 8,697,359, US 8,771,945, US 8,795,965, US 8,865,406, US 8,871,445, US 8,889,356, US 8,889,418, US 8,895,308, US 8,906,616 and foreign equivalents (“Patents”). PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
