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ThawSTAR® CFT2 Automated Thawing System

Automated cell thawing system for consistent thawing performance
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产品优势


  • Obtain reproducible thawing profiles using a standardized thawing method

  • Eliminate risk of contamination from water bath-based thawing techniques

  • Thaw cells directly in the biosafety cabinet

  • Portable solution for the handling and transport of frozen vials

  • Convenient and compact format

  • Document instrument performance and generate an audit trail with ThawSTAR® CFT2 confirmation vials

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    概述
    实验数据
    产品说明书及文档
    应用领域
    相关材料与文献
    Increase confidence in your cell thawing workflow and ensure sample sterility and consistent thawing performance by using the ThawSTAR® CFT2 Automated Thawing System. With a standardized thawing process that replaces manual, water bath-based thawing, ThawSTAR® eliminates the risk of contamination and delivers controlled thawing profiles. Using this convenient and compact system, you can thaw your cells directly in the biosafety cabinet. Simply insert a frozen cryogenic vial and retrieve it once the device alerts you at the end of the thaw cycle, after approximately 2.5 minutes. The ThawSTAR® CFT2 Automated Thawing System can be leveraged early in the R&D phase and scaled into commercial manufacturing.

    During vial transport, protect your cells from transient warming events and ensure maximum cell health by using the ThawSTAR® CFT2 Transporter. It provides a portable solution for the handling and transport of frozen vials, from long-term storage in vapor phase liquid nitrogen or in a -80°C freezer, to downstream processing with the ThawSTAR® CFT2 Automated Thawing System.

    ThawSTAR® CFT2 Automated Thawing System is compatible with a variety of 1.8 - 2.0 mL vials.

    Note: For performance qualification documentation and accessories required to facilitate functional testing, use the ThawSTAR® CFT2 IOPQ Kit (available for separate purchase). Additional ThawSTAR® CFT2 Confirmation Vials to evaluate the performance of the instrument are also available for purchase.

    Data Figures

    Bar graphs comparing the number of live cells recovered and percentage viability for peripheral blood mononuclear cells (PBMCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

    Figure 1. Frozen PBMCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

    Peripheral blood mononuclear cells (PBMCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 3 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.78 x 107 vs. 2.96 x 107 cells, respectively and (B) the mean viability was 92.8% vs. 93.9%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

    Bar graphs comparing the number of live cells recovered and percentage viability for monocytes thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

    Figure 2. Frozen Monocytes Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

    Monocytes cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 2 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.05 x 107 vs. 1.89 x 107 cells, respectively and (B) the mean viability was 95% vs. 94.6%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

    Bar graphs comparing the number of live cells recovered and percentage viability for human pluripotent stem cells (hPSCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

    Figure 3. Frozen hPSCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

    Human pluripotent stem cells (hPSCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 1 x 106 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 9.05 x 105 vs. 9.35 x 105 cells, respectively and (B) the mean viability was 83.04% vs. 82.93%, respectively. The hPSCs were from 3 different cell lines (M001, 1C, and H9) and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

    Protocols and Documentation

    Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

    Document Type
    Product Name
    Catalog #
    Lot #
    Language
    Document Type
    Product Information Sheet 1
    Product Name
    ThawSTAR® CFT2 Automated Thawing System
    Catalog #
    100-0650
    Lot #
    All
    Language
    English
    Document Type
    Product Information Sheet 2
    Product Name
    ThawSTAR® CFT2 Automated Thawing System
    Catalog #
    100-0650
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet
    Product Name
    ThawSTAR® CFT2 Automated Thawing System
    Catalog #
    100-0650
    Lot #
    All
    Language
    English

    Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    Research Area
    Workflow Stages
    PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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