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ThawSTAR® CFT2全自动细胞解冻系统

全自动细胞解冻系统，确保一致的解冻性能
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产品号 #100-0650_C

全自动细胞解冻系统，确保一致的解冻性能

产品优势

  • 使用标准化的解冻方案获得可重复的解冻曲线
  • 避免水浴解冻方案带来的污染风险
  • 可在生物安全柜中直接解冻细胞
  • 配有处理和运输冻存管的便携式解决方案
  • 方便、小巧、灵活度高
  • 利用ThawSTAR® CFT2测试样品可记录仪器性能并生成审计跟踪

产品组分包括

  • Thawstar® CFT2全自动细胞解冻系统，1台（产品号 #100-0640）
  • ThawSTAR® CFT2转运盒，1个（产品号 #100-0641）
  • ThawSTAR® CFT2电源和适配器（产品号 #100-0644）
  • ThawSTAR® CFT2测试样品管，2管
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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

"通过使用ThawSTAR® CFT2全自动细胞解冻系统，提高细胞解冻实验成功率，确保样品无菌性且解冻性能一致性高。ThawSTAR®采用标准化解冻流程，取代手动水浴解冻，避免了样品污染的风险，并提供受控的解冻曲线。这款方便紧凑的全自动细胞解冻系统，允许用户直接在生物安全柜里解冻细胞。只需插入冻存管，并在解冻周期结束时（大约2.5分钟）设备发出提示音后取出即可。ThawSTAR® CFT2全自动细胞解冻系统可以在研发阶段早期使用，并放大到到商业化生产中。

在解冻样品运输过程中，使用ThawSTAR® CFT2转运盒保护细胞免受瞬时升温事件的影响，并确保细胞处于最优的健康状态。它为处理和运输冻存管提供了便携式解决方案，适用于从长期储存在气相液氮或-80°C冰箱中，到使用ThawSTAR® CFT2全自动细胞解冻系统进行下游处理。

ThawSTAR® CFT2全自动细胞解冻系统与多种1.8 - 2.0 mL冻存管兼容。

注：如需性能认证文件和功能测试所需的配件，请使用ThawSTAR® CFT2 IOPQ检测试剂盒（可单独购买）。此外，用于评估仪器性能的ThawSTAR® CFT2测试样品管也可单独购买。"

应用
冻存
 
品牌
ThawSTAR
 
研究领域
脐带血库，免疫
 

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Data Figures

Bar graphs comparing the number of live cells recovered and percentage viability for peripheral blood mononuclear cells (PBMCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 1. Frozen PBMCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Peripheral blood mononuclear cells (PBMCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 3 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.78 x 107 vs. 2.96 x 107 cells, respectively and (B) the mean viability was 92.8% vs. 93.9%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for monocytes thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 2. Frozen Monocytes Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Monocytes cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 2 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.05 x 107 vs. 1.89 x 107 cells, respectively and (B) the mean viability was 95% vs. 94.6%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for human pluripotent stem cells (hPSCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 3. Frozen hPSCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Human pluripotent stem cells (hPSCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 1 x 106 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 9.05 x 105 vs. 9.35 x 105 cells, respectively and (B) the mean viability was 83.04% vs. 82.93%, respectively. The hPSCs were from 3 different cell lines (M001, 1C, and H9) and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
ThawSTAR® CFT2 Automated Thawing System
Catalog #
100-0650
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
ThawSTAR® CFT2 Automated Thawing System
Catalog #
100-0650
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ThawSTAR® CFT2 Automated Thawing System
Catalog #
100-0650
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
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