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STEMdiff™前脑神经元分化试剂盒

分化试剂盒用于从人胚胎干细胞和iPS细胞衍生的神经祖细胞生成神经前体细胞

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产品号 #（选择产品）

产品号 #08600_C

分化试剂盒用于从人胚胎干细胞和iPS细胞衍生的神经祖细胞生成神经前体细胞

产品优势

支持从hpsc衍生的神经元前体高效生成功能神经元
产生高纯度（≥90%神经元）的混合兴奋性和抑制性神经元，可在培养中长期维持
STEMdiff™SMADi神经诱导试剂盒优化神经祖细胞分化
支持神经元活动的生理学相关结果
使来自多种人类胚胎干细胞和iPS细胞系的神经元前体可再生成熟

产品组分包括

STEMdiff™前脑神经元分化基础培养基，80 mL
STEMdiff™前脑神经元分化补充液，20ml

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Need a high-quality cell source? Use the hiPSC SCTi003-A (female) or SCTi004-A (male) control lines, manufactured with mTeSR™ Plus.

To see all required products for your protocol, please consult the Protocols and Documentation.

STEMdiff™ Neural Rosette Selection Reagent
Enzyme-free reagent for the selective detachment of neural rosettes
STEMdiff™ SMADi Neural Induction Kit
Serum-free medium kit for highly efficient SMAD inhibition-mediated neural induction of human ES and iPS cells
STEMdiff™ Forebrain Neuron Maturation Kit
Maturation kit for generation of functional neurons from human ES and iPS cell-derived neuronal precursor cells
Human iPSC-Derived Neural Progenitor Cells
Frozen human neural progenitor cells differentiated from the human induced pluripotent stem cell line, SCTi003-A
DMEM/F-12 with 15 mM HEPES
Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

总览

实验数据

产品说明书及文档

应用领域

概述

STEMdiff™前脑神经元分化试剂盒与STEMdiff™前脑神经元成熟试剂盒（目录号08605）一起使用，从人类多能干细胞衍生的神经祖细胞中产生混合的前脑型（foxg1阳性）神经元。该试剂盒经过优化，可与STEMdiff™SMADi神经诱导试剂盒配合使用，STEMdiff™SMADi神经诱导试剂盒可提供合适的神经祖细胞。使用这些产品衍生的神经元是模拟人类神经发育和疾病、药物筛选、毒性测试和细胞治疗验证的通用工具。

Subtype
Specialized Media

Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells

Species
Human

Application
Cell Culture, Differentiation

Brand
STEMdiff

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience

Formulation Category
Serum-Free

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Data Figures

Figure 1. Schematic for the Embryoid Body Protocol

Forebrain-type neural precursors can be generated in 18 - 19 days from hPSC-derived NPCs after selecting neural rosettes from replated embryoid bodies. For the maturation of precursors to forebrain-type neurons, see the PIS. hPSC = human pluripotent stem cell; NPCs = neural progenitor cells; PIS = product information sheet

Figure 2. Schematic for the Monolayer Protocol

Forebrain-type neural precursors can be generated from NPC monolayers derived from embryonic and induced pluripotent stem cells after three single-cell passages. For the maturation of precursors to forebrain-type neurons, see the PIS. NPC = neural progenitor cell; PIS = product information sheet

Figure 3. Forebrain-Type Neurons Are Generated After Culture in STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits

NPCs generated from hPSCs in mTeSR 1™ using the STEMdiff™ SMADi Neural Induction Kit EB protocol were differentiated and matured to forebrain-type neurons using the STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits. (A) Forebrain-type neurons were formed after iPS cell-derived NPCs were cultured with the STEMdiff™ Forebrain Neuron Differentiation Kit for 7 days and STEMdiff™ Forebrain Neuron Maturation Kit for 14 days. The resulting cultures contain a highly pure population of (B) class III β-tubulin-positive neurons (green), with (C) fewer than 10% astrocytes (GFAP-positive cells, red). (D) Nuclei are labeled with DAPI (blue). NPCs = neural progenitor cells; hPSC = human pluripotent stem cell; EB = embryoid body; iPS = induced pluripotent stem

Figure 4. Downstream Differentiation of Neural Progenitor Cells to Neurons Is Possible Using the STEMdiff™ Differentiation and Maturation Kits

(A) NPCs generated from STiPS-R038 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit EB protocol were differentiated and matured to cortical neurons using STEMdiff™ Forebrain Neuron Differentiation Kit for 7 days and STEMdiff™ Forebrain Neuron Maturation Kit for 14 days. The resulting cultures contain a highly pure population of (B) class III β-tubulin-positive neurons (green) with less than 10% GFAP-positive astrocytes (not shown). (C) The generated neurons are also positive for FOXG1 expression (red), indicating a forebrain-type identity. (D) Nuclei are labeled with Hoechst (blue). NPCs = neural progenitor cells; hPSC = human pluripotent stem cell

Figure 5. A Mixed Population of Forebrain-Type Cortical Neurons Is Generated Using the STEMdiff™ Differentiation and Maturation Kits

Forebrain-type neurons generated from iPSC-derived NPCs (line AIW002-02) were cultured using the STEMdiff™ Forebrain Neuron Differentiation Kit for 7 days and subsequently matured for the following 6 weeks using STEMdiff™ Forebrain Neuron Maturation Kit. The resulting cultures contain a mixed population of neurons expressing VGLUT1, a glutamatergic marker of excitatory neurons (green), as well as MAP2-positive neurons, indicating mature neurons (magenta). Nuclei are labeled with Hoechst (blue). Data courtesy of Cecilia Rocha, The Neuro's Early Drug Discovery Unit (EDDU), McGill University. iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells

Figure 6. PSC-Derived Astrocytes and Neurons Can Be Co-Cultured to Model Cell-Cell Interactions In Vitro

NPCs generated from the H1 cell line were differentiated to astrocytes using STEMdiff™ Astrocyte Differentiation and Maturation Kits. H9 cell-derived NPCs were differentiated to forebrain-type neurons using STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits. For co-culture, matured astrocytes were seeded onto forebrain neurons that had been in STEMdiff™ Forebrain Neuron Maturation Medium for at least one week. Co-cultures were then switched to STEMdiff™ Forebrain Neuron Maturation Medium the following day and for the remaining co-culture. (A) Neurons cultured alone, following the co-culture feeding schedule, are labeled with DCX (green). (B) DCX-positive neurons (green) and astrocytes (GFAP, red) can be co-cultured for at least 1 - 2 weeks prior to analysis.For a detailed co-culture protocol, please see the Methods Library. NPCs = neural progenitor cells

Figure 7. PSC-Derived Neurons Survive and Mature when Co-Cultured with PSC-Derived Astrocytes

NPCs generated from the STiPS-R038 cell line were differentiated to astrocytes using STEMdiff™ Astrocyte Differentiation and Maturation Kits. STiPS-M001 cell-derived NPCs were differentiated to forebrain-type neurons using STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits. After co-culture for one week, neurons (A) had significantly increased neurite outgrowth as measured on MAP2-positive neurons with the NeuriteTracer plugin for ImageJ (M Pool et al. J Neurosci Methods, 2008) and (B) were more numerous than neurons cultured alone using the same feeding schedule. *, p < 0.05; **, p < 0.01. NPCs = neural progenitor cells

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

STEMdiff™ Forebrain Neuron Differentiation Kit

Catalog #

08600

Lot #

All

Language

English

Document Type

Safety Data Sheet 1

Product Name

STEMdiff™ Forebrain Neuron Differentiation Kit

Catalog #

08600

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

STEMdiff™ Forebrain Neuron Differentiation Kit

Catalog #

08600

Lot #

All

Language

English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area

Workflow Stages

Workflow Stages for Human Pluripotent Stem Cell Research

Reprogramming

Maintenance

Differentiation

Workflow Stages for hPSC-Derived Neural Cell Research

Cell Sourcing

Neural Induction

Expansion

Differentiation and Maturation

Characterization