CELL TYPE T Cells, T Cells, CD4+, T Cells, Regulatory
SPECIES Human
SAMPLE SOURCE Leukapheresis, PBMC
SELECTION METHOD Positive
APPLICATION Cell Isolation
BRAND EasySep, RoboSep
AREA OF INTEREST Immunology
实验数据
Figure 1. Typical EasySep™ Human Regulatory T Cell Isolation Profile When Processing Up to 1x 10^9 Cells Per Isolation
Starting with fresh or previously frozen PBMCs, the regulatory T cell content (CD4+CD25+FOXP3+) of the isolated fraction is typically 85.0 ± 4.8% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 1.8% and 88.2%, respectively.
EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit Protocol
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