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SepMate™-15 (RUO)

密度梯度离心管，仅供研究使用
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产品号 #（选择产品）

产品号 #86415_C

密度梯度离心管，仅供研究使用

产品优势

  • 无需缓慢而费力地将血液加于密度梯度离心液面（例如 Ficoll-Paque™、Lymphoprep™）
  • 对于新鲜样本，将离心总时间缩短至 10 分钟，并无需关闭刹车下进行
  • 只需倾倒上清液即可快速轻松地收集分离的单核细胞
  • 可与RosetteSep™富集试剂配合使用，仅需25分钟即可分离特定细胞类型

产品组分包括

  • SepMate™-15（RUO），100支装（产品号#86415）
    • 分装盒含四包管，每包25支
  • SepMate™-15（RUO），500支装（产品号#86420）
    • 分装盒含四包管，每包25支（产品号#86415）×5
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Try SepMate™-15 tubes for your density gradient centrifugation. Request a Sample
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

SepMate™ 是一种用于通过密度梯度离心法分离外周血单个核细胞（PBMCs）或特定细胞类型的管子。SepMate™管包含一个插件，作为将血液与离心液分隔开的屏障，让您无需仔细将血液（或骨髓、脐带血）铺在离心液层上。该设计使得单个核细胞的分离过程更加快速便捷，仅需离心后简单倾倒即可收集PBMCs。SepMate™ 可与 RosetteSep™ 富集试剂联合使用，可在 30 分钟内实现特定细胞类型的快速分离。

SepMate™-15 适用于处理 0.5 至 5 mL 的样本。

SepMate™-15 (RUO)（产品号 #86415 和 #86420）的生产符合现行药品生产质量管理规范（cGMP），仅供科研使用（RUO）。

查阅SepMate™常见问题解答(FAQs)。

包含
Polypropylene tube containing an insert
 
亚型
离心管
 
细胞类型
B 细胞，树突状细胞（DCs），单核细胞，单个核细胞，NK 细胞，T 细胞，T 细胞，CD4+，T 细胞，CD8+，T 细胞，其他亚群，T 细胞，调节性细胞
 
种属
人
 
样本来源
Bone Marrow，Cord Blood，Whole Blood
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
SepMate
 
研究领域
嵌合体，HLA，免疫
 

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Data Figures

PBMC recovery from fresh whole blood using SepMate™-50 versus standard density gradient centrifugation. Graph also shows PBMC recovery from a 48 hour-old sample using SepMate™. n in each group = 7

Figure 1. Recovery of mononuclear cells (MNCs) from peripheral blood using SepMate™-50 versus standard density gradient centrifiguation. Recovery of MNCs from fresh and 48-hour post blood draw enriched by density gradient centrifugation with SepMate™ (purple) or without (grey). There was no significant difference in the recovery of MNCS with and without SepMate™.

PBMC recovery from fresh whole blood using SepMate™-50 versus standard density gradient centrifugation. Graph also shows PBMC recovery from a 48 hour-old sample using SepMate™. n in each group = 7

Figure 2. Human CD4+ T Cell Isolation using SepMate™-50 and RosetteSep™ Human CD4+ T Cell Enrichment Cocktail

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
SepMate™-15 (RUO)
Catalog #
86415, 86420
Lot #
All
Language
English

Resources and Publications

Educational Materials (9)

SepMate™ Hassle-Free PBMCs in Just 15 Minutes
Brochure
SepMate™ Hassle-Free PBMCs in Just 15 Minutes
How to Isolate Mononuclear Cells from Blood in 15 Minutes with SepMate™
Protocol
How to Isolate Mononuclear Cells from Blood in 15 Minutes with SepMate™
How SepMate™ PBMC Isolation Tubes Work: From Whole Blood to PBMCs in 15 Minutes
2:28
Video
How SepMate™ PBMC Isolation Tubes Work: From Whole Blood to PBMCs in 15 Minutes
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...
Cell Isolation in One Spin Using SepMate™ Tubes and RosetteSep™ Cell Separation Reagents
1:02
Video
Cell Isolation in One Spin Using SepMate™ Tubes and RosetteSep™ Cell Separation Reagents
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
1:46
Video
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
A Specialized Tube to Make Rosettesep™ Enrichment of Specific Cell Subsets Faster and Easier
Scientific Poster
A Specialized Tube to Make Rosettesep™ Enrichment of Specific Cell Subsets Faster and Easier
A Specialized Tube to Make Enrichment of Specific Cell Subsets Faster and Easier
Scientific Poster
A Specialized Tube to Make Enrichment of Specific Cell Subsets Faster and Easier
A Specialized Tube for Rapid PBMC Preparation, Pre-Enrichment, or Isolation of Specific Cell Populations
Scientific Poster
A Specialized Tube for Rapid PBMC Preparation, Pre-Enrichment, or Isolation of Specific Cell Populat...

Publications (16)

A Comprehensive Structure-Function Study of Neurogenin3 Disease-Causing Alleles during Human Pancreas and Intestinal Organoid Development. X. Zhang et al. Developmental cell 2019 aug

Abstract

Neurogenin3 (NEUROG3) is required for endocrine lineage formation of the pancreas and intestine. Patients with NEUROG3 mutations are born with congenital malabsorptive diarrhea due to complete loss of enteroendocrine cells, whereas endocrine pancreas development varies in an allele-specific manner. These findings suggest a context-dependent requirement for NEUROG3 in pancreas versus intestine. We utilized human tissue differentiated from NEUROG3-/- pluripotent stem cells for functional analyses. Most disease-associated alleles had hypomorphic or null phenotype in both tissues, whereas the S171fsX68 mutation had reduced activity in the pancreas but largely null in the intestine. Biochemical studies revealed NEUROG3 variants have distinct molecular defects with altered protein stability, DNA binding, and gene transcription. Moreover, NEUROG3 was highly unstable in the intestinal epithelium, explaining the enhanced sensitivity of intestinal defects relative to the pancreas. These studies emphasize that studies of human mutations in the endogenous tissue context may be required to assess structure-function relationships.
View publication
Oral administration of hydroxylated-graphene quantum dots induces intestinal injury accompanying the loss of intestinal stem cells and proliferative progenitor cells. L. Yu et al. Nanotoxicology 2019

Abstract

Graphene quantum dots (GQDs) have gained significant attention in various biomedical applications. The physicochemical properties of these nanoparticles, including toxic effects, are largely determined by their surface modifications. Previous studies have demonstrated high in vitro cytotoxicity of the hydroxylated GQDs (OH-GQDs). The focus of this study was on the intestinal toxicity of OH-GQDs. Briefly, C57BL/6J mice were given daily oral gavage of 0.05, 0.5 or 5 mg/kg OH-GQD for 7 days, and the indices of intestinal damage were evaluated. Higher doses of the OH-GQDs caused significant intestinal injuries, such as enhanced intestinal permeability, shortened villi and crypt loss. The number of Lgr5+ intestinal stem cells also decreased dramatically upon OH-GQDs exposure, which also inhibited the Ki67+ proliferative progenitor cells. In addition, an increased number of crypt cells harboring the oxidized DNA base 8-OHdG and $\gamma$H2AX foci were also detected in the intestines of OH-GQD-treated mice. Mechanistically, the OH-GQDs up-regulated both total and phosphorylated p53. Consistent with this, the average number of TUNEL+ and cleaved caspase-3+ apoptotic intestinal epithelial cells were significantly increased after OH-GQDs treatment. Finally, a 3-dimensional organoid culture was established using isolated crypts, and OH-GQDs treatment significantly reduced the size of the surviving intestinal organoids. Taken together, the intestinal toxicity of the OH-GQDs should be taken into account during biomedical applications.
View publication
IL-27 amplifies cytokine responses to Gram-negative bacterial products and Salmonella typhimurium infection. C. Petes et al. Scientific Reports 2018 SEP

Abstract

Cytokine responses from monocytes and macrophages exposed to bacteria are of particular importance in innate immunity. Focusing on the impact of the immunoregulatory cytokine interleukin (IL)-27 on control of innate immune system responses, we examined human immune responses to bacterial products and bacterial infection by E. coli and S. typhimurium. Since the effect of IL-27 treatment in human myeloid cells infected with bacteria is understudied, we treated human monocytes and macrophages with IL-27 and either LPS, flagellin, or bacteria, to investigate the effect on inflammatory signaling and cytokine responses. We determined that simultaneous stimulation with IL-27 and LPS derived from E. coli or S. typhimurium resulted in enhanced IL-12p40, TNF-$\alpha$, and IL-6 expression compared to that by LPS alone. To elucidate if IL-27 manipulated the cellular response to infection with bacteria, we infected IL-27 treated human macrophages with S. typhimurium. While IL-27 did not affect susceptibility to S. typhimurium infection or S. typhimurium-induced cell death, IL-27 significantly enhanced proinflammatory cytokine production in infected cells. Taken together, we highlight a role for IL-27 in modulating innate immune responses to bacterial infection.
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更多信息

更多信息
Species Human
Contains Polypropylene tube containing an insert
Sample Source Bone Marrow, Cord Blood, Whole Blood
Selection Method Negative
Legal Statement: Ficoll-Paque™ PLUS is a trademark of GE Healthcare Ltd.Lymphoprep™ is a trademark of AXIS-SHIELD. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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