切换导航
  • 比较产品
我的购物车

抗人CD4抗体，克隆OKT4

小鼠单克隆IgG2b抗体，抗人、恒河猴、食蟹猴CD4
率先评论此产品
添加并比较

产品号 #（选择产品）

产品号 #60016_C

小鼠单克隆IgG2b抗体，抗人、恒河猴、食蟹猴CD4

main product photo
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

OKT4抗体识别CD4，CD4是一种~59 kDa的单链1型跨膜糖蛋白，是免疫球蛋白（Ig）超家族的成员。CD4包含4个细胞外Ig样结构域（D1 - D4）。OKT4的表位定位于该蛋白的D3结构域，其结构类似于Ig可变区。CD4在大多数胸腺细胞和一些T细胞亚群（辅助T细胞）中表达水平相对较高，而在外周血单核细胞和巨噬细胞中表达水平较低。CD4结合MHC II的非多态性区域，并通过增强TCR与MHC II-抗原复合物之间结合的亲和力，在MHC II限制性抗原识别中作为T细胞受体（TCR）的共受体。CD4还通过胞内结构域与细胞质酪氨酸激酶（如Lck）的相互作用，放大从TCR到胞质的信号。此外，CD4是人类免疫缺陷病毒（HIV）的受体。OKT4抗体与CD4的结合不能阻断HIV结合。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™Direct人CD4+ T细胞分选试剂盒（产品号 #19662），EasySep™人CD4+ T细胞富集试剂盒（产品号 #19052），EasySep™人CD3正选试剂盒II（产品号 #17851）和EasySep™人CD4正选试剂盒II（产品号 #17852）分选的细胞纯度。

亚型
一抗
 
靶抗原
CD4
 
别名
T4
 
活性物种
Chimpanzee，Cynomolgus，人，Rhesus，Sooty Mangabey
 
偶联
Alexa Fluor 488，APC，Biotin 或 生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的
 
宿主物种
小鼠
 
细胞类型
T 细胞，T 细胞，CD4+，T 细胞，调节性细胞
 
种属
人，非人灵长类
 
应用
流式细胞术，功能学筛选，免疫荧光，免疫组化，免疫沉淀
 
研究领域
免疫，移植研究
 
克隆
OKT4
 
基因编号
920
 
同种型
IgG2b，kappa
 

查看更多 显示更少

Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone OKT4, Alexa Fluor® 488 and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human PBMCs isolated with the EasySep™ Human CD3 Positive Selection Kit and labeled with Anti-Human CD4 Antibody, Clone OKT4, Alexa Fluor® 488. Histograms show labeling of total PBMCs (Start) and isolated cells (Isolated). Labeling with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488 (Catalog #60072AD) is shown in the bottom panel (solid line histogram). (C) Flow cytometry analysis of human PBMCs isolated with the EasySep™ Human CD4 Positive Selection Kit and labeled with Anti-Human CD4 Antibody, Clone OKT4, Alexa Fluor® 488. Histograms show labeling of total PBMCs (Start) and isolated cells (Isolated). Labeling with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488 is shown in the bottom panel (solid line histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone OKT4, APC and Anti-Human CD45 Antibody, Clone HI30, FITC (Catalog #60018FI). (B) Flow cytometry analysis of human PBMCs labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, APC (Catalog #60072AZ) and Anti-Human CD45 Antibody, Clone HI30, FITC.

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone OKT4, Biotin followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Biotin (Catalog #60072BT), followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone OKT4, FITC and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human PBMCs labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, FITC (Catalog #60072FI) and Anti-Human CD45 Antibody, Clone HI30, APC.

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone OKT4, PE and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human PBMCs isolated with the EasySep™ Human CD4 Positive Selection Kit and labeled with Anti-Human CD4 Antibody, Clone OKT4, PE. Histograms show labeling of total PBMCs (Start) and isolated cells (Isolated). Labeling with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE (Catalog #60072PE) is shown (solid line histogram).

Data for Unconjugated

Figure 6. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone OKT4, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11 (Catalog #60072), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone OKT4, PerCP-Cy5.5 (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PerCP-Cy5.5 (Catalog #60072PS; solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD4 Antibody, Clone OKT4, Alexa Fluor® 488
Catalog #
60016AD, 60016AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD4 Antibody, Clone OKT4, APC
Catalog #
60016AZ, 60016AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD4 Antibody, Clone OKT4, Biotin
Catalog #
60016BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD4 Antibody, Clone OKT4, FITC
Catalog #
60016FI, 60016FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD4 Antibody, Clone OKT4, PE
Catalog #
60016PE, 60016PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD4 Antibody, Clone OKT4, PerCP-Cy5.5
Catalog #
60016PS, 60016PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD4 Antibody, Clone OKT4
Catalog #
60016
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD4 Antibody, Clone OKT4, Alexa Fluor® 488
Catalog #
60016AD, 60016AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD4 Antibody, Clone OKT4, APC
Catalog #
60016AZ, 60016AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD4 Antibody, Clone OKT4, Biotin
Catalog #
60016BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD4 Antibody, Clone OKT4, FITC
Catalog #
60016FI, 60016FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD4 Antibody, Clone OKT4, PE
Catalog #
60016PE, 60016PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD4 Antibody, Clone OKT4, PerCP-Cy5.5
Catalog #
60016PS, 60016PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD4 Antibody, Clone OKT4
Catalog #
60016
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

更多信息

更多信息
Species Human, Non-Human Primate
Clone OKT4
Gene Id 920
Alternative Names T4
Isotype IgG2b, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
版权 © 2025 STEMCELL Technologies 技术有限公司。保留所有权利。