EasySep™小鼠CD11c正选试剂盒II

免疫磁珠正选小鼠CD11c+细胞

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产品号 #（选择产品）

产品号 #18780_C

免疫磁珠正选小鼠CD11c+细胞

产品优势

操作简单、快捷，且无需分离柱
纯度高达95%

产品组分包括

EasySep™小鼠CD11c 正选试剂盒II（产品号 #18780）

EasySep™小鼠CD11c 正选试剂盒II组分A, 0.5 mL

EasySep™小鼠CD11c 正选试剂盒II组分B, 0.5 mL

EasySep™ Dextran RapidSpheres™ 50100 磁珠, 2 x 1 mL

EasySep™小鼠FcR阻断剂（产品号 #18731），0.5 mL

RoboSep™ 小鼠CD11c 正选试剂盒II（产品号 #18780RF）

EasySep™小鼠CD11c 正选试剂盒II组分A, 0.5 mL

EasySep™小鼠CD11c 正选试剂盒II组分B, 0.5 mL

EasySep™ Dextran RapidSpheres™ 50100 磁珠, 2 x 1 mL

EasySep™小鼠FcR阻断剂（产品号 #18731），0.5 mL

RoboSep™ 空管

RoboSep™ 缓冲液（产品号 #20104）

RoboSep™过滤吸头（产品号 #20125）

EasySep™小鼠CD11c正选试剂盒II及脾脏解离液(产品号 #18781)

EasySep™小鼠CD11c 正选试剂盒II组分A, 0.5 mL

EasySep™小鼠CD11c 正选试剂盒II组分B, 0.5 mL

EasySep™ Dextran RapidSpheres™ 50100 磁珠, 2 x 1 mL

EasySep™小鼠FcR阻断剂（产品号 #18731），0.5 mL

脾脏解离液（产品号 #07915）。

RoboSep™ 小鼠CD11c正选试剂盒II及脾脏解离液 (产品号#18781RF)

EasySep™小鼠CD11c 正选试剂盒II组分A, 0.5 mL

EasySep™小鼠CD11c 正选试剂盒II组分B, 0.5 mL

EasySep™ Dextran RapidSpheres™ 50100 磁珠, 2 x 1 mL

EasySep™小鼠FcR阻断剂（产品号#18731），0.5 mL

RoboSep™ 空管

脾脏解离液（产品号 #07915）。

RoboSep™ 缓冲液（产品号 #20104）

RoboSep™过滤吸头（产品号 #20125）

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总览

使用EasySep™小鼠CD11c正选试剂盒II，可轻松从小鼠脾细胞或培养的骨髓细胞样本中通过免疫磁珠正选获得高纯度的小鼠CD11c+细胞。EasySep™技术结合单克隆抗体的特异性和免磁柱系统的简便性，已在发表的研究中广泛应用超过20年。

在该 EasySep™正选流程中，通过识别CD11c的抗体复合物及磁珠标记目的细胞。使用EasySep™磁极分离标记细胞，只需倾倒或移除非目的细胞即可。目的细胞将保留在流式管中。免疫磁珠分选获得的 CD11c+细胞可用于下游应用，例如流式细胞术、细胞培养和其它基于细胞的实验。

了解更多EasySep™免疫磁珠技术的工作原理，或者如何通过RoboSep™实现全自动化免疫磁珠细胞分选。探索其他针对您的工作流程优化的产品，包括培养基、补充剂、抗体等。

Data Figures

Figure 1. Typical EasySep™ CD11c Positive Cell Isolation Profile

Starting with mouse splenocytes, the CD11c+ cell content of the enriched fraction is typically 86.8 ± 9.7% (gated on viable singlet cells, mean ± SD) using the purple EasySep™ Magnet. In the example above, the final purities of the start and isolated fraction are 5.7% and 92.3%, respectively.

Figure 2. FACS Data for Anti-Mouse CD11c Antibody, Clone N418, Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes processed with EasySep™ Mouse CD11c Positive Selection Kit II (Catalog #18780) and labeled with Anti-Mouse CD11c Antibody, Clone N418, Alexa Fluor® 488 (Catalog # 60002AD). Histograms show labeling of splenocytes (Start) and isolated cells (Isolated). Labeling of the start cells with an Armenian hamster IgG Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (solid line histogram).

(B) Flow cytometry analysis of C57BL/6 mouse splenocytes processed with EasySep™ Mouse CD11c Positive Selection Kit II and labeled with Anti-Mouse CD11c Antibody, Clone N418, Alexa Fluor® 488 and an anti-mouse CD317 antibody, APC.

(C) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11c Antibody, Clone N418, Alexa Fluor® 488 and an anti-mouse MHC class II antibody, APC.

(D) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with an Armenian hamster IgG Alexa Fluor® 488 isotype control antibody and an anti-mouse MHC class II antibody, APC.

Educational Materials (8)

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x10⁸ cells/mL and a minimum working volume of 100 µL. Samples containing 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

Ethanol Intoxication and Burn Injury Increases Intestinal Regulatory T Cell Population and Regulatory T Cell Suppressive Capability. M. E. Luck et al. Shock (Augusta, Ga.) 2022 feb

Abstract

Traumatic injuries, such as burn, are often complicated by ethanol intoxication at the time of injury. This leads to a myriad of complications and post-burn pathologies exacerbated by aberrant immune responses. Recent findings suggest that immune cell dysfunction in the gastrointestinal system is particularly important in deleterious outcomes associated with burn injuries. In particular, intoxication at the time of burn injury leads to compromised intestinal T cell responses, which can diminish intestinal immunity and promote bacterial translocation, allowing for increased secondary infections in the injured host and associated sequelae, such as multiple organ failure and sepsis. Regulatory T cells (Treg) have been identified as important mediators of suppressing effector T cell function. Therefore, the goal of this study was to assess the effects of ethanol intoxication and burn injury on Treg populations in small intestinal immune organs. We also evaluated the suppressive capability of Tregs isolated from injured animals. Male C57BL/6 mice were gavaged with 2.9?Šg/kg ethanol before receiving a ˆ¼12.5% total body surface area scald burn. One day after injury, we identified a significant increase in Tregs number in small intestine Peyer's patches (ˆ¼?—1.5) and lamina propria (ˆ¼?—2). Tregs-producing cytokine IL-10 were also increased in both tissues. Finally, Tregs isolated from ethanol and burn-injured mice were able to suppress proliferation of effector T cells to a greater degree than sham vehicle Tregs. This was accompanied by increased levels of IL-10 and decreased levels of pro-proliferative cytokine IL-2 in cultures containing ethanol + burn Tregs compared with sham Tregs. These findings suggest that Treg populations are increased in intestinal tissues 1 day following ethanol intoxication and burn injury. Tregs isolated from ethanol and burn-injured animals also exhibit a greater suppression of effector T cell proliferation, which may contribute to altered T cell responses following injury.

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Characterization of the Immunologic Phenotype of Dendritic Cells Infected With Herpes Simplex Virus 1. J. Zhang et al. Frontiers in immunology 2022

Abstract

Due to viral envelope glycoprotein D binding to cellular membrane HVEM receptor, HSV-1 can infect certain dendritic cells, which becomes an event in the viral strategy to interfere with the host's immune system. We previously generated the HSV-1 mutant strain M6, which produced an attenuated phenotype in mice and rhesus monkeys. The attenuated M6 strain was used to investigate how HSV-1 infection of dendritic cells interferes with both innate and adaptive immunity. Our study showed that dendritic cells membrane HVEM receptors could mediate infection of the wild-type strain and attenuated M6 strain and that dendritic cells infected by both viruses in local tissues of animals exhibited changes in transcriptional profiles associated with innate immune and inflammatory responses. The infection of pDCs and cDCs by the two strains promoted cell differentiation to the CD103+ phenotype, but varied transcriptional profiles were observed, implying a strategy that the HSV-1 wild-type strain interferes with antiviral immunity, probably due to viral modification of the immunological phenotype of dendritic cells during processing and presentation of antigen to T cells, leading to a series of deviations in immune responses, ultimately generating the deficient immune phenotype observed in infected individuals in the clinical.

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PDE4B Is a Homeostatic Regulator of Cyclic AMP in Dendritic Cells. A. M. Chinn et al. Frontiers in pharmacology 2022

Abstract

Chronic decreases in the second messenger cyclic AMP (cAMP) occur in numerous settings, but how cells compensate for such decreases is unknown. We have used a unique system-murine dendritic cells (DCs) with a DC-selective depletion of the heterotrimeric GTP binding protein G$\alpha$s-to address this issue. These mice spontaneously develop Th2-allergic asthma and their DCs have persistently lower cAMP levels. We found that phosphodiesterase 4B (PDE4B) is the primary phosphodiesterase expressed in DCs and that its expression is preferentially decreased in G$\alpha$s-depleted DCs. PDE4B expression is dynamic, falling and rising in a protein kinase A-dependent manner with decreased and increased cAMP concentrations, respectively. Treatment of DCs that drive enhanced Th2 immunity with a PDE4B inhibitor ameliorated DC-induced helper T cell response. We conclude that PDE4B is a homeostatic regulator of cellular cAMP concentrations in DCs and may be a target for treating Th2-allergic asthma and other settings with low cellular cAMP concentrations.

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Species	Mouse
Magnet Compatibility	• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
Sample Source	Bone Marrow, Other, Spleen
Selection Method	Positive

EasySep™小鼠CD11c正选试剂盒II

产品号 #（选择产品）

产品号 #18780_C

产品优势

产品组分包括

总览

Data Figures

Protocols and Documentation

Applications

Resources and Publications

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Scientists Helping Scientists™

EasySep™小鼠CD11c正选试剂盒II

产品号 #（选择产品）

产品号 #18780_C

产品优势

产品组分包括

总览

Data Figures

Protocols and Documentation

Applications

Resources and Publications

Educational Materials (8)

Frequently Asked Questions

Can EasySep™ be used for either positive or negative selection?

How does the separation work?

Which columns do I use?

How can I analyze the purity of my enriched sample?

Can EasySep™ separations be automated?

Can EasySep™ be used to isolate rare cells?

Are the EasySep™ magnetic particles FACS-compatible?

Can the EasySep™ magnetic particles be removed after enrichment?

Can I alter the separation time in the magnet?

For positive selection, can I perform more than 3 separations to increase purity?

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Publications (7)

Abstract

Abstract

Abstract

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