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抗小鼠CD11b抗体，克隆M1/70

抗人、小鼠、恒河猴CD11b的大鼠单克隆IgG2b抗体
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产品号 #60001_C

抗人、小鼠、恒河猴CD11b的大鼠单克隆IgG2b抗体

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总览
实验数据
产品说明书及文档
应用领域
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总览

M1/70抗体可特异性识别CD11b，CD11b是一种分子量约170 kDa的1型跨膜糖蛋白，与CD18非共价结合形成异二聚体Mac-1受体。Mac-1通过结合ICAM-1（CD54）、ICAM-2（CD102）、ICAM-4（CD242）、iC3b和纤维蛋白原等配体，在多个过程中发挥作用，包括介导中性粒细胞/单核细胞与活化内皮细胞的粘附以及促进补体包被颗粒的吞噬作用。CD11b表达于粒细胞、单核细胞、NK细胞、树突状细胞、组织巨噬细胞以及T细胞和B细胞亚群的表面，并被用作区分naïve CD8+ T细胞和记忆CD8+ T细胞的标志物。CD11b是一个相对较晚的髓系分化标志物，在大多数髓系单核细胞造血祖细胞和更初始的细胞中检测不到。研究发现，M1/70抗体可抑制iC3b与Mac-1的结合。该抗体克隆已验证可用于通过EasySep™试剂盒（包括EasySep™小鼠单核细胞分选试剂盒，产品号 #19861）分离的细胞的纯度检测。

亚型
一抗
 
靶抗原
CD11b
 
别名
alphaM整合素，C3biR，CR3，Ly-40，Mac-1，Mo1
 
活性物种
Baboon，Chimpanzee，人，兔，Rhesus
 
偶联
Biotin 或 生物素，FITC，PE，未偶联的
 
宿主物种
大鼠
 
细胞类型
粒细胞及其亚群，单核细胞
 
种属
人，小鼠，非人灵长类，其它细胞系
 
应用
细胞分选，CyTOF，FACS，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，放射免疫分析，Western印迹
 
研究领域
免疫
 
克隆
M1/70
 
基因编号
16409
 
同种型
IgG2b，kappa
 

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Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488 and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody and anti-mouse CD45 APC.
(C) Flow cytometry analysis of C57BL/6 mouse splenocytes processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488. Histograms show labeling of splenocytes (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).
(D) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488. Histograms show labeling of bone marrow cells (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PE and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa PE isotype control antibody and anti-mouse CD45 APC.
(C) Flow cytometry analysis of C57BL/6 mouse splenocytes processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PE. Histograms show labeling of splenocytes (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa PE isotype control antibody is shown in the bottom panel (open histogram).
(D) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PE. Histograms show labeling of bone marrow cells (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa PE isotype control antibody is shown in the bottom panel (open histogram).

Data for unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD11b Antibody, Clone M1/70, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or a rat IgG2b, kappa isotype control antibody followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE).
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa APC isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Biotin followed by streptavidin (SAV) APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE).
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa biotin isotype control antibody followed by SAV APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ).
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa FITC isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Figure 8. Data for PB-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Pacific Blue™ and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa Pacific Blue™ isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488
Catalog #
60001AD, 60001AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, APC
Catalog #
60001AZ, 60001AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Biotin
Catalog #
100-0439, 100-0438, 60001BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, FITC
Catalog #
100-0437, 100-0436, 60001FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Pacific Blue™
Catalog #
60001PB, 60001PB.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, PE
Catalog #
100-0435, 100-0434
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, PerCP-Cy5.5
Catalog #
60001PS, 60001PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70
Catalog #
100-0433
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488
Catalog #
60001AD, 60001AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, APC
Catalog #
60001AZ, 60001AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Biotin
Catalog #
100-0439, 100-0438, 60001BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, FITC
Catalog #
100-0437, 100-0436, 60001FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Pacific Blue™
Catalog #
60001PB, 60001PB.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, PE
Catalog #
100-0435, 100-0434
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, PerCP-Cy5.5
Catalog #
60001PS, 60001PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70
Catalog #
100-0433
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human, Mouse, Non-Human Primate, Other
Clone M1/70
Gene Id 16409
Alternative Names alphaM integrin, C3biR, CR3, Ly-40, Mac-1, Mo1
Isotype IgG2b, kappa
Legal Statement: Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® or Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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