产品号 #78066_C
声波刺猬
Sonic hedgehog (Shh)是hedgehog家族的一员,是一种分泌信号蛋白,在哺乳动物早期发育过程中具有重要的模式作用(Ho & Scott)。它在胚胎发育早期在多种组织中表达(Finco等人)。在中枢神经系统的模式化过程中,Shh直接作用于细胞,指定神经细胞的命运,调节少突胶质前体细胞和神经嵴细胞的增殖和存活,刺激增殖、分化和轴突生长(Ho & Scott;马蒂)。Shh也调节T细胞的分化和激活(Crompton等人)。重组小鼠Shh是一种具有完全生物活性的分子。
Subtype
Cytokines
Cell Type
Neural Stem and Progenitor Cells, Pluripotent Stem Cells
Species
Mouse
Area of Interest
Neuroscience, Stem Cell Biology
Purity
≥ 95%
(A) The biological activity of Mouse Recombinant Shh was tested by its ability to promote alkaline phosphatase production of CCL-226 cells. Alkaline phosphatase production was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.8 μg/mL.
(B) 2 μg of Mouse Recombinant Shh was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant Shh has a predicted molecular mass of 19.8 kDa.
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Species | Mouse |
---|---|
Purity | ≥ 95% |
白介素4
白血病抑制因子
‘诺金’
声波刺猬
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