产品号 #78047_C
Interleukin 4
概述
Interleukin 4 (IL-4) is a type I cytokine produced by Th2 T cells, NK T cells, γ/δ T cells, eosinophils, mast cells, and activated basophils. IL-4 receptors are present on a number of cells, including hematopoietic, endothelial, epithelial, muscle, and brain tissues. IL-4 receptor engagement initiates signaling through the JAK/STAT pathway, and leads to the activation of PI3K and Ras/MAPK pathways (Nelms et al.). IL-4 plays a key role in immunoglobulin class switching in B cells and in regulating the differentiation of naïve T cells into T helper 2 (Th2) cells, while inhibiting Th1 differentiation. IL-4 acts together with tumor necrosis factor to stimulate expression of adhesion molecules on vascular endothelial cells and to downregulate expression of E-selectin, thus recruiting T cells and eosinophils to the site of inflammation (Nelms et al.).
Subtype
Cytokines
Cell Type
B Cells, Dendritic Cells, Hematopoietic Stem and Progenitor Cells, Lymphocytes, Monocytes, Myeloid Cells, Neurons, T Cells
Species
Mouse
Area of Interest
Immunology, Neuroscience, Stem Cell Biology
Purity
≥ 95%
Data Figures
(A) The biological activity of Mouse Recombinant IL-4 was tested by its ability to promote the proliferation of HT-2 cells. Cell proliferation was measured after 67 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 3.8 - 5.7 ng/mL.
(B) 1 μg of Mouse Recombinant IL-4 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant IL-4 has a predicted molecular mass of 13.7 kDa.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Mouse Recombinant IL-4 Catalog #
78047, 78047.2, 78047.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Mouse Recombinant IL-4 Catalog #
78047, 78047.2, 78047.1 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Workflow Stages for Dendritic Cell Research
Workflow Stages for Hematopoietic Stem and Progenitor Cell Research
Workflow Stages for Total Lymphocyte Research
Workflow Stages for Monocyte Research
Workflow Stages for Myeloid Cell Research
Resources and Publications
Educational Materials (3)
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