产品号 #02038_C
用于小鼠白细胞介素4的检测和测定
概述
小鼠白细胞介素4 （IL-4）酶联免疫吸附测定试剂盒是专为定量检测和测量小鼠白细胞介素4在生物液体，如血清，血浆和细胞培养上清。IL-4由Th2细胞、NK细胞、γ/δ T细胞、嗜酸性细胞、肥大细胞和活化的嗜碱性细胞产生。IL-4诱导B细胞中的Ig同型转换，调节naïve T细胞向Th2细胞的分化，并与TNF一起将T细胞和嗜酸性粒细胞募集到炎症部位。
该检测基于夹心ELISA方法，将样品添加到酶联免疫吸附试验（ELISA）带材板上，该带材板上预先涂有捕获的细胞因子特异性抗体。捕获的细胞因子通过添加生物素化检测抗体进行检测，然后添加链亲和素-辣根过氧化物酶（SA-HRP），该酶与生物素化抗体结合。添加显色酶底物3,3 ‘,5,5 ’四甲基联苯胺（TMB）可得到着色产物，其强度与样品中细胞因子的浓度成正比。细胞因子的浓度是通过与平行分析的细胞因子标准品的连续稀释进行比较来确定的。
Subtype
Complete Kits
Cell Type
Granulocytes and Subsets, T Cells
Species
Mouse
Area of Interest
Immunology
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (2)
更多信息
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
