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抗大鼠CD90抗体，克隆OX-7

小鼠单克隆IgG1抗体，抗小鼠、大鼠、豚鼠CD90/CD90.1
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产品号 #60024_C

小鼠单克隆IgG1抗体，抗小鼠、大鼠、豚鼠CD90/CD90.1

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总览
实验数据
产品说明书及文档
应用领域
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总览

OX-7抗体识别大鼠CD90 （Thy-1）和小鼠CD90.1 （Thy-1.1），后者是小鼠品系AKR/J、PL和FVB/N表达的CD90等位形式。OX-7抗体不能识别CD90.2（在许多小鼠品系中表达，包括CBA和BALB/c）。CD90是一种GPI连接的膜糖蛋白，是免疫球蛋白超家族的成员。25 kDa的核心蛋白在三个位点被N-糖基化，产生分子量范围在25 - 37 kDa的分子。在大鼠中，CD90在多种细胞类型中表达，包括造血干细胞、未成熟B细胞、胸腺细胞和神经元。在表达CD90.1的小鼠品系中，在骨髓的早期造血细胞、胸腺细胞和循环成熟T细胞中皆发现了CD90.1。OX-7抗体据报导可诱导白细胞活化、肾小球肾炎、肾小球系膜细胞凋亡和血管通透性。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™小鼠T细胞分选试剂盒（产品号 #19851）从兼容的小鼠品系中分选的细胞纯度。

亚型
一抗
 
靶抗原
CD90/CD90.1
 
别名
Thy-1，Thy-1.1
 
活性物种
Guinea Pig，小鼠，兔，大鼠
 
偶联
Alexa Fluor 488，APC，Biotin 或 生物素，FITC，PE，未偶联的
 
宿主物种
小鼠
 
细胞类型
T 细胞
 
种属
小鼠，其它细胞系，大鼠
 
应用
流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
 
研究领域
免疫
 
克隆
OX-7
 
基因编号
21838/24832
 
同种型
IgG1，kappa
 

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Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of Sprague-Dawley rat brain cells labeled with Anti-Rat CD90 Antibody, Clone OX-7, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).
(B) Flow cytometry analysis of Sprague-Dawley rat thymocytes labeled with Anti-Rat CD90 Antibody, Clone OX-7, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of Sprague-Dawley rat brain cells labeled with Anti-Rat CD90 Antibody, Clone OX-7, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).
(B) Flow cytometry analysis of Sprague-Dawley rat thymocytes labeled with Anti-Rat CD90 Antibody, Clone OX-7, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

(A) Flow cytometry analysis of Sprague-Dawley rat brain cells labeled with Anti-Rat CD90 Antibody, Clone OX-7, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram). (B) Flow cytometry analysis of Sprague-Dawley rat thymocytes labeled with Anti-Rat CD90 Antibody, Clone OX-7, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of Sprague-Dawley rat brain cells labeled with Anti-Rat CD90 Antibody, Clone OX-7, APC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60070AZ) (solid line histogram). (B) Flow cytometry analysis of Sprague-Dawley rat thymocytes labeled with Anti-Rat CD90 Antibody, Clone OX-7, APC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of Sprague-Dawley rat brain cells labeled with Anti-Rat CD90 Antibody, Clone OX-7, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin (Catalog #60070BT), followed by SAV APC (solid line histogram). (B) Flow cytometry analysis of Sprague-Dawley rat thymocytes labeled with Anti-Rat CD90 Antibody, Clone OX-7, Biotin, followed by SAV APC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin, followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

(A) Flow cytometry analysis of Sprague-Dawley rat brain cells labeled with Anti-Rat CD90 Antibody, Clone OX-7, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI) (solid line histogram). (B) Flow cytometry analysis of Sprague-Dawley rat thymocytes labeled with Anti-Rat CD90 Antibody, Clone OX-7, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7, Alexa Fluor® 488
Catalog #
60024AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7, APC
Catalog #
60024AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7, FITC
Catalog #
60024FI.1, 60024FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX­7, PE
Catalog #
60024PE, 60024PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7
Catalog #
60024
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7, Alexa Fluor® 488
Catalog #
60024AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7, APC
Catalog #
60024AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7, FITC
Catalog #
60024FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7, FITC
Catalog #
60024FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX­7, PE
Catalog #
60024PE, 60024PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7
Catalog #
60024
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (3)

Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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更多信息
Species Mouse, Other, Rat
Clone OX-7
Gene Id 21838/24832
Alternative Names Thy-1, Thy-1.1
Isotype IgG1, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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