人重组IL-4, ACF

白细胞介素4,不含动物成分

产品号 #(选择产品)

产品号 #78147_C

白细胞介素4,不含动物成分

总览

人白细胞介素4(IL-4)对蠕虫感染的免疫反应以及过敏反应至关重要(Olpihant et al.)。IL-4受体由IL-4Ra异二聚体和共同的γ链组成。IL-4受体结合可激活JAK1/3并募集STAT6和IRS1/2(Nelms et al.)。IL-4驱动B细胞免疫球蛋白类别转换(IgG4转换为 IgE、IgG4)、肥大细胞增生、粘液产生以及naïve T细胞分化为辅助性T细胞2(Th2),后者可产生IL-4、 IL-5、 IL-10和IL-13 (Bao et al.; Olpihant et al.; Nelms et al.)。除Th2 T细胞外,IL-4还可由CD4+ NK T细胞、γ/δ T细胞、活化的嗜碱性粒细胞、嗜酸性粒细胞和肥大细胞产生。IL-4由130个氨基酸组成,预计分子量为15.1 kDa。人IL-4不与小鼠细胞发生交叉反应(Park et al.)。本产品不含动物成分。

亚型
细胞因子
 
细胞类型
B 细胞,树突状细胞(DCs),造血干/祖细胞,淋巴细胞,单核细胞,髓系细胞,神经元,T 细胞
 
种属

 
研究领域
免疫,神经科学,干细胞生物学
 
制剂类别
Animal Component-Free
 
纯度
≥ 95 %
 

Data Figures

(A) The biological activity of Human Recombinant IL-4, ACF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured after 72 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.2 ng/mL. (B) 1 μg of Human Recombinant IL-4, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78147, 78147.1, 78147.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78147, 78147.1, 78147.2
Lot #
All
Language
English

更多信息

更多信息
Species Human
Formulation Category Animal Component-Free
Purity ≥ 95%
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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