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人ipsc衍生的间充质祖细胞

人诱导多能干细胞系SCTi003-A分化的冻存的间充质祖细胞
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产品号 #200-0781_C

人诱导多能干细胞系SCTi003-A分化的冻存的间充质祖细胞

产品优势

  • 节省时间，使用经过严格鉴定的间充质祖细胞中间群开启实验
  • 通过经充分鉴定的iPSC标准细胞系SCTi003-A分化获得高品质MPCs
  • 解冻后可直接培养，无需复苏培养步骤
  • 兼容MesenCult™分化系统，可定向分化为脂肪细胞、成骨细胞或软骨细胞
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Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Animal Component-Free Cell Dissociation Kit
    Animal Component-Free Cell Dissociation Kit

    Dissociation kit for human stem and progenitor cells

  2. MesenCult™-ACF Plus Culture Kit
    MesenCult™-ACF Plus Culture Kit

    Animal component-free medium and attachment substrate for human mesenchymal stem cells

  3. MesenCult™-ACF Freezing Medium
    MesenCult™-ACF Freezing Medium

    Animal component-free MSC cryopreservation medium

  4. L-Glutamine
    L-Glutamine

    Cell culture supplement

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品
选择高品质、即用型人iPSC来源间充质祖细胞（MPCs），助您轻松开启间充质细胞培养实验。本产品为冻存型多能祖细胞，由健康女性供体外周血单个核细胞 (PBMCs) 来源的人诱导多能干细胞 (iPSC) 标准细胞系SCTi003-A分化获得。解冻后可直接使用，配合MesenCult™培养基体系可实现稳定培养和定向分化。MPCs能高效分化为间充质谱系细胞：如脂肪细胞（使用MesenCult™成脂分化试剂盒[人])）、成骨细胞（使用MesenCult™成骨分化试剂盒[人]）和软骨细胞（使用MesenCult™成软骨分化试剂盒[人]）。

为确保高质量标准，人iPSC衍生的MPCs是采用STEMdiff™间充质祖细胞试剂盒从SCTi003-A细胞分化获得。所得MPCs高表达CD73、CD90和CD105等间充质细胞标志物，在使用MesenCult™-ACF Plus培养基（包含于MesenCult™-ACF Plus培养试剂盒）培养时能够实现长期扩增。这些MPCs还可使用MesenCult™-ACF冻存液进行低温保存，为您的实验进程提供灵活性。MPCs及其分化的脂肪细胞、成骨细胞和软骨细胞，可用于再生医学开发、药物发现和疾病建模研究。

本产品仅供研究使用 (RUO) ，已获得学术和商业使用授权。原始血样采集均通过机构审查委员会 (IRB) 或其他监管机构批准的知情同意书和方案进行，确保符合伦理规范。源细胞库的供体详细信息和细胞质量表征数据请参阅本页面图表。更多详情请参考iPSC细胞系常见问题解答。
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Data Figures

Figure 1

Figure 1. Human iPSC-Derived Mesenchymal Progenitor Cells Show High-Quality Morphology and Robust Expansion for Multiple Passages

(A) Representative microscopy image showing cryopreserved Human iPSC-Derived Mesenchymal Progenitor Cells thawed and maintained in MesenCult™-ACF Plus Medium for 4 days and ready for passaging at ~80% confluency. (B) Cells show robust expansion post-thaw. Data points show the average doubling time over 3 passages post-thaw from 3 technical replicates. C) Human iPSC-Derived Mesenchymal Progenitor Cells were thawed and maintained in MesenCult™-ACF Plus Medium. Cells were passaged every 3-4 days post thaw and cell expansion was analyzed as cumulative cell number over 4 passages from 2 technical replicates.

Figure 2

Figure 2. Human iPSC-Derived Mesenchymal Progenitor Cells Express Characteristic Markers

Human iPSC-Derived Mesenchymal Progenitor Cells were thawed, cultured in MesenCult™-ACF Plus Medium for one passage, characterized using flow cytometry for mesenchymal cell markers, and show high expression of CD73, CD90, and CD105. (A) Percentage marker expression was quantified from the analyses of five technical replicates. Representative flow cytometry plots are displayed for (B) CD73, (C) CD90 and (D) CD105.

Figure 3

Figure 3. Human iPSC-Derived Mesenchymal Progenitor Cells Can Differentiate into Adipocytes, Osteoblasts, and Chondrocytes

(A) Adipocytes were generated from Human iPSC-Derived Mesenchymal Progenitor Cells using MesenCult™ Adipogenic Differentiation Kit. Adipogenic differentiation was assessed by Oil Red O stain at Day 30 post differentiation. (B) Osteoblasts were differentiated using MesenCult™ Osteogenic Differentiation Kit. Osteogenic differentiation was assessed by Alizarin Red S stain at Day 21 post differentiation. (C) Chondrocytes were differentiated using MesenCult™-ACF Chondrogenic Differentiation Kit. Chondrogenic differentiation was assessed by Alcian Blue and Nuclear Fast Red Stain at Day 21 post differentiation.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Human iPSC-Derived Mesenchymal Progenitor Cells
Catalog #
200-0781
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (1)

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Species Human
Contains CryoStor® CS10
Purity ≥ 90% CD73+CD90+CD105+ cells by flow cytometry
Cell And Tissue Source Pluripotent Stem Cells
Donor Status Normal
Legal Statement: These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) shall not be used or administered in (1) human subjects for human clinical use; (2) animals for veterinary use for therapeutic, diagnostic, or prophylactic purposes or (3) any subject in relation to clinical applications, cell therapy, transplantation, and/or regenerative medicines, without limiting the generality of the foregoing. These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for monetization or commercialization purposes, including without limitation, used to, or with the goal to, perform services or supply products or rights, including in the manufacture of cellular therapies or other therapeutics, for monetary gain or the generation of royalties, revenues, sales or other valuable consideration. For clarity, these iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for screening compounds, antibodies, proteins or peptides, except for the purposes of target discovery, target validation, or assay development, provided such activities and the results of such activities are not further used for monetization or commercialization purposes. It may be possible to obtain a further license for the prohibited uses referred to in this Limited Use License. Please contact iPSCrequests@stemcell.com for more details. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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