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MSC表征抗体面板

人间充质间质细胞多色流式细胞仪
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产品号 #（选择产品）

产品号 #100-0354_C

人间充质间质细胞多色流式细胞仪

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Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Round-Bottom Polystyrene Tubes with Caps, 5 mL
    Round-Bottom Polystyrene Tubes with Caps, 5 m...

    Sterile polystyrene round-bottom tubes; 5mL with caps

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

  3. Corning® 96-Well Round-Bottom Microplate
    Corning® 96-Well Round-Bottom Microplate

    Clear polystyrene round-bottom untreated microplate with lid

  4. Anti-Human CD32 Antibody, Clone IV.3
    Anti-Human CD32 Antibody, Clone IV.3

    Mouse monoclonal IgG2b antibody against human CD32

  5. DAPI (Hydrochloride)
    DAPI (Hydrochloride)

    DNA-labeling dye

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概述

MSC表征抗体面板设计用于人间充质间质细胞（MSCs）的表型分析。本试剂盒包含三种荧光染料偶联抗体，用于鉴定人间充质干细胞，以及一种荧光染料偶联抗体，用于鉴定非间充质干细胞。当流式细胞术测量时，≥95%的间充质干细胞群体必须表达CD73、CD90和CD105，并且这些细胞必须缺乏CD45的表达（≤2%阳性）。这些试剂为验证人间充质干细胞提供单步标记。MSC表征抗体面板可用于鉴定来自各种组织的人间充质干细胞，如骨髓间充质干细胞、多能干细胞来源的间充质祖细胞、脂肪来源的间充质干细胞、牙髓来源的间充质干细胞和脐带来源的间充质干细胞。

Subtype
Primary Antibodies
 
Cell Type
Mesenchymal Stromal Cells
 
Application
Flow Cytometry
 
Area of Interest
Endothelial Cell Biology, Stem Cell Biology
 

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Data Figures

Flow cytometry dot plots comparing the difference in expression of CD45, CD73, CD90, and CD105 in human bone marrow-derived mesenchymal stromal cells (MSCs) labeled with the hMSC CD45/CD73/CD90/CD105 Antibody Panel and cultured either in MesenCult™-ACF Plus Medium or in serum-containing medium.

Figure 1. Flow Cytometry Analysis of Human Bone Marrow-Derived Mesenchymal Stromal Cells Labeled Using the hMSC CD45/CD73/CD90/CD105 Antibody Panel

(A) When cultured in MesenCult™-ACF Plus Medium, the labeled human bone marrow (BM)-derived mesenchymal stromal cells (MSCs), demonstrated positive expression of CD73, CD105, and CD90 as well as negative expression of CD45 at P0. (B) When cultured in serum-containing medium, the BM-derived MSCs exhibit reduced CD73, CD105, and CD90 expression as well as increased CD45 expression at P0 due to contaminating CD45+ cells.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
MSC Characterization Antibody Panel
Catalog #
100-0354
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
MSC Characterization Antibody Panel
Catalog #
100-0354
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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Workflow Stages

Resources and Publications

Educational Materials (2)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
MesenCult™-ACF Plus
Brochure
MesenCult™-ACF Plus
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