Medium for the differentiation of human MSCs into adipocytes
产品优势
Robust and versatile human MSC differentiation to adipocytes
Optimized for differentiation of bone marrow- and adipose tissue-derived human MSCs previously cultured in serum-containing or serum-free media, such as MesenCult™-ACF Plus Medium
Compatible with human MSCs previously cultured in platelet lysate media
产品组分包括
MesenCult™ MSC Basal Medium (Human), 225 mL
MesenCult™ 10X Adipogenic Differentiation Supplement (Human), 25 mL
MesenCult™ 500X Adipogenic Differentiation Supplement (Human), 0.5 mL
MesenCult™ Adipogenic Differentiation Medium (Human) is specifically formulated for the in vitro differentiation of primary human mesenchymal stromal cells, and hPSC-derived mesenchymal progenitor cells (also known as mesenchymal stem cells or MSCs) into adipogenic lineage cells. This kit is suitable for the differentiation of MSCs derived from human bone marrow (BM), adipose tissue, umbilical cord, or pluripotent stem cells (PSCs) that have been previously culture-expanded in serum- and animal component-free medium (e.g. MesenCult™ ACF Plus Medium [Catalog #05445]), serum-containing medium (e.g. MesenCult™ Proliferation Kit [Catalog #05411]), or platelet lysate medium (e.g. MesenCult™-hPL Medium [Catalog #05439]).
Subtype Specialized Media
Cell Type Adipocytes, Mesenchymal Stem and Progenitor Cells
Species Human
Application Cell Culture, Differentiation
Brand MesenCult
Area of Interest Stem Cell Biology
Data Figures
Figure 1. Adipogenic Differentiation of Human Bone Marrow-Derived MSCs
Adipogenic differentiation of human bone marrow-derived MSCs using MesenCult™ Adipogenic Differentiation Medium (Human) or a competitor medium. Prior to differentiation, MSCs were cultured for 2 passages in either serum- and xeno-free media (MesenCult™-XF or a 10% platelet lysate-based formulation) or serum-containing medium (MesenCult™) before undergoing differentiation. Even though differentation results are donor dependent, MesenCult™ Adipogenic Differentiation Medium (Human) consistently performed as well as, or better than the competitor medium. This trend was consistent for MSCs previously cultured in MesenCult™-XF, 10% Platelet Lysate or MesenCult™ medium.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Differential expression of drug resistance genes in CD146 positive dental pulp derived stem cells and CD146 negative fibroblasts. M. S. Tavangar et al. Clinical and experimental dental research 2020 may
Abstract
INTRODUCTION The stem cell portion of the dental pulp derived cultures (DPSCs) showed a higher resistance to cytotoxic effect of restorative dental materials compared to pulpal fibroblasts (DPFs). Here, we aimed to compare the expression of some drug resistant genes between these cells. METHODS AND MATERIALS To separate DPSCs from DPFs, we used magnetic cell sorting technique based on CD146 expression. To assess the stem cell properties, the positive and negative portions underwent colony forming assays and were induced to be differentiated into the adipocytes, osteoblasts, hepatocytes, and neural cells. Cell surface antigen panels were checked using immune fluorescence and flow-cytometry techniques. The mRNA expression of 14 ABC transporters including ABCA2, ABCB1, ABCB11, ABCC1, ABCC2, ABCC3, ABCC4, ABCC5-2, ABCC5-4,ABCC5-13, ABCC6, ABCC10, ABCC11, and ABCG2 genes was assessed, using quantitative RT-PCR technique. RESULTS Only the CD146 positive portion could be differentiated into the desired fates, and they formed higher colonies (16.7 ± 3.32 vs. 1.7 ± 1.67, p {\textless} .001). The cell surface antigen panels were the same, except for CD146 and STRO-1 markers which were expressed only in the positive portion. Among the ABC transporter genes studied, the positive portion showed a higher expression (approximately two-fold) of ABCA2, ABCC5-13, and ABCC5-2 genes. CONCLUSION Dental pulp stem cells which can be separated from dental pulp fibroblasts based on CD146 expression, express higher levels of some drug resistance genes which probably accounts for their features of more resistance to cytotoxic effects of some dental materials. This needs to be more validated in future.
In situ repair abilities of human umbilical cord-derived mesenchymal stem cells and autocrosslinked hyaluronic acid gel complex in rhesus monkeys with intrauterine adhesion. L. Wang et al. Science advances 2020 may
Abstract
Increasing occurrence of moderate to severe intrauterine adhesion (IUA) is seriously affecting the quality of human life. The aim of the study was to establish IUA models in nonhuman primates and to explore the dual repair effects of human umbilical cord-derived mesenchymal stem cells (huMSCs) loaded on autocrosslinked hyaluronic acid gel (HA-GEL) on endometrial damage and adhesion. Here, we recorded the menstrual cycle data in detail with uterine cavities observed and endometrial tissues detected after intervention, and the thicker endometria, decreased amount of fibrotic formation, increased number of endometrium glands, etc., suggested that both HA-GEL and huMSC/HA-GEL complexes could partially repair IUA caused by mechanical injury, but huMSC/HA-GEL complex transplantation had notable dual repair effects: a reliable antiadhesion property and the promotion of endometrial regeneration.
Intravenous administration of iPS-MSCSPIONs mobilized into CKD parenchyma and effectively preserved residual renal function in CKD rat. J.-J. Sheu et al. Journal of cellular and molecular medicine 2020 mar
Abstract
This study traced intravenously administered induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (MSC) and assessed the impact of iPSC-MSC on preserving renal function in SD rat after 5/6 nephrectomy. The results of in vitro study showed that FeraTrack™Direct contrast particles (ie intracellular magnetic labelling) in the iPSC-MSC (ie iPS-MSCSPIONs ) were clearly identified by Prussian blue stain. Adult-male SD rats (n = 40) were categorized into group 1 (SC), group 2 [SC + iPS-MSCSPIONs (1.0 × 106 cells)/intravenous administration post-day-14 CKD procedure], group 3 (CKD), group 4 [CKD + iPS-MSCSPIONs (0.5 × 106 cells)] and group 5 [CKD + iPS-MSCSPIONs (1.0 × 106 cells)]. By day-15 after CKD induction, abdominal MRI demonstrated that iPS-MSCSPIONs were only in the CKD parenchyma of groups 4 and 5. By day 60, the creatinine level/ratio of urine protein to urine creatinine/kidney injury score (by haematoxylin and eosin stain)/fibrotic area (Masson's trichrome stain)/IF microscopic finding of kidney injury molecule-1 expression was lowest in groups 1 and 2, highest in group 3, and significantly higher in group 4 than in group 5, whereas IF microscopic findings of podocyte components (ZO-1/synaptopodin) and protein levels of anti-apoptosis ((Bad/Bcl-xL/Bcl-2) exhibited an opposite pattern to creatinine level among the five groups (all P {\textless} .0001). The protein expressions of cell-proliferation signals (PI3K/p-Akt/m-TOR, p-ERK1/2, FOXO1/GSK3$\beta$/p90RSK), apoptotic/DNA-damage (Bax/caspases8-10/cytosolic-mitochondria) and inflammatory (TNF-$\alpha$/TNFR1/TRAF2/NF-$\kappa$B) biomarkers displayed an identical pattern to creatinine level among the five groups (all P {\textless} .0001). The iPS-MSCSPIONs that were identified only in CKD parenchyma effectively protected the kidney against CKD injury.
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