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EasySep™人嗜酸性粒细胞分选试剂盒

从新鲜人多形核细胞 (PMNCs) 中免疫磁珠负选人嗜酸性粒细胞（CD16-CD66b+CD45+）
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产品号 #（选择产品）

产品号 #17956_C

从新鲜人多形核细胞 (PMNCs) 中免疫磁珠负选人嗜酸性粒细胞（CD16-CD66b+CD45+）

产品优势

  • 操作简单、快捷，且无需分离柱
  • 纯度高达99%
  • 获得的活细胞无标记

产品组分包括

  • EasySep™人嗜酸性粒细胞分选试剂盒（产品号 #17956）
    • EasySep™人嗜酸性粒细胞分选抗体混合物，1 mL
    • EasySep™ Dextran RapidSpheres™磁珠，1 mL
  • RoboSep™ 人嗜酸性粒细胞分选试剂盒（含过滤吸头）（产品号 #17956RF）
    • EasySep™人嗜酸性粒细胞分选抗体混合物，1 mL
    • EasySep™ Dextran RapidSpheres™磁珠，1 mL
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号 #20125）
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  1. EasySep™磁铁
    EasySep™磁铁
  2. EasySep™缓冲液
    EasySep™缓冲液
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

使用EasySep™ 人嗜酸性粒细胞分选试剂盒，通过免疫磁珠负选，可轻松高效地从新鲜人外周血多形核细胞（PMNCs）中分离高纯度嗜酸性粒细胞（CD16-CD66b+CD45+）。EasySep™技术结合单克隆抗体的特异性和无柱磁分选系统的简便性，已在发表的研究中广泛应用超过20年。

在该EasySep™负选流程中，非目标细胞被抗体复合物与磁珠标记。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着简单地将目的细胞倾倒或吸取至一个新的试管中。磁珠分选后，目的嗜酸性粒细胞可直接用于下游应用，如流式细胞术、细胞培养或DNA/RNA提取。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化。探索更多为您实验流程优化的其它产品，包括培养基、补充剂、抗体等。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
粒细胞及其亚群
 
种属
人
 
样本来源
PMNC，Whole Blood
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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Data Figures

Figure 1. Typical EasySep™ Human Eosinophil Isolation Profile

Starting with whole blood prepared using RBC lysis or HetaSep™, the eosinophil content (CD16-CD66b+CD45+) of the isolated fraction is typically 96.5 ± 2.5% (mean ± SD) using the purple EasySep™ Magnet. In the above examples, the purities of the start and final isolated fractions prepared using RBC lysis are 1.8% and 97.8% (gated on CD45+), respectively, and when prepared using HetaSep™, the purities are 2.4% and 96.8% (gated on CD45+), respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
RoboSep™ Human Eosinophil Isolation Kit
Catalog #
17956RF
Lot #
All
Language
French
Document Type
Product Information Sheet 2
Product Name
RoboSep™ Human Eosinophil Isolation Kit
Catalog #
17956RF
Lot #
All
Language
English
Document Type
Product Information Sheet 1
Product Name
EasySep™ Human Eosinophil Isolation Kit
Catalog #
17956
Lot #
All
Language
French
Document Type
Product Information Sheet 2
Product Name
EasySep™ Human Eosinophil Isolation Kit
Catalog #
17956
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Eosinophil Isolation Kit
Catalog #
17956RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Eosinophil Isolation Kit
Catalog #
17956RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human Eosinophil Isolation Kit
Catalog #
17956RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Eosinophil Isolation Kit
Catalog #
17956
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Eosinophil Isolation Kit
Catalog #
17956
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

更多信息

更多信息
Species Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
Sample Source PMNC, Whole Blood
Selection Method Negative
标记抗体
  1. 抗人CD16抗体，克隆号3G8
    抗人CD16抗体，克隆号3G8

    小鼠抗人、恒河猴、食蟹猴CD16单克隆IgG1抗体

  2. 抗人CD66b抗体，克隆G10F5
    抗人CD66b抗体，克隆G10F5

    小鼠单克隆IgM抗体，抗人、黑猩猩CD66b

  3. 抗人CD11b抗体，克隆ICRF44
    抗人CD11b抗体，克隆ICRF44

    抗人、恒河猴、食蟹猴CD11b的小鼠单克隆IgG1抗体

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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