产品号 #19659_C
全血免疫磁珠负选试剂盒
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总览
EasySep™ Direct 人Pan-粒细胞分选试剂盒通过免疫磁珠负选，直接从人全血中分离具有功能的、高纯度的中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞。无需裂解、密度梯度离心或其他处理步骤，分选后的细胞可直接用于流式细胞术、功能分析、细胞培养及其他下游应用。
磁体兼容性
• EasySep™ Magnet (Catalog #18000)，or
• “The Big Easy” EasySep™ Magnet (Catalog #18001)，or
• Easy 50 EasySep™ Magnet (Catalog #18002)，or
• EasyEights™ EasySep™ Magnet (Catalog #18103)
亚型
细胞分选试剂盒
细胞类型
粒细胞及其亚群
种属
人
样本来源
Whole Blood
筛选方法
Negative
应用
细胞分选
品牌
EasySep
研究领域
免疫
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (8)
Frequently Asked Questions
Publications (2)
Nuclear positioning facilitates amoeboid migration along the path of least resistance. Nature 2019 apr
Abstract
During metazoan development, immune surveillance and cancer dissemination, cells migrate in complex three-dimensional microenvironments1-3. These spaces are crowded by cells and extracellular matrix, generating mazes with differently sized gaps that are typically smaller than the diameter of the migrating cell4,5. Most mesenchymal and epithelial cells and some-but not all-cancer cells actively generate their migratory path using pericellular tissue proteolysis6. By contrast, amoeboid cells such as leukocytes use non-destructive strategies of locomotion7, raising the question how these extremely fast cells navigate through dense tissues. Here we reveal that leukocytes sample their immediate vicinity for large pore sizes, and are thereby able to choose the path of least resistance. This allows them to circumnavigate local obstacles while effectively following global directional cues such as chemotactic gradients. Pore-size discrimination is facilitated by frontward positioning of the nucleus, which enables the cells to use their bulkiest compartment as a mechanical gauge. Once the nucleus and the closely associated microtubule organizing centre pass the largest pore, cytoplasmic protrusions still lingering in smaller pores are retracted. These retractions are coordinated by dynamic microtubules; when microtubules are disrupted, migrating cells lose coherence and frequently fragment into migratory cytoplasmic pieces. As nuclear positioning in front of the microtubule organizing centre is a typical feature of amoeboid migration, our findings link the fundamental organization of cellular polarity to the strategy of locomotion.
High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry. Nature communications 2019
Abstract
In life sciences, the material properties of suspended cells have attained significance close to that of fluorescent markers but with the advantage of label-free and unbiased sample characterization. Until recently, cell rheological measurements were either limited by acquisition throughput, excessive post processing, or low-throughput real-time analysis. Real-time deformability cytometry expanded the application of mechanical cell assays to fast on-the-fly phenotyping of large sample sizes, but has been restricted to single material parameters as the Young's modulus. Here, we introduce dynamic real-time deformability cytometry for comprehensive cell rheological measurements at up to 100 cells per second. Utilizing Fourier decomposition, our microfluidic method is able to disentangle cell response to complex hydrodynamic stress distributions and to determine viscoelastic parameters independent of cell shape. We demonstrate the application of our technology for peripheral blood cells in whole blood samples including the discrimination of B- and CD4+ T-lymphocytes by cell rheological properties.
更多信息
| Species | Human |
|---|---|
| Magnet Compatibility | • EasySep™ Magnet (Catalog #18000), or • “The Big Easy” EasySep™ Magnet (Catalog #18001), or • Easy 50 EasySep™ Magnet (Catalog #18002), or • EasyEights™ EasySep™ Magnet (Catalog #18103) |
| Sample Source | Whole Blood |
| Selection Method | Negative |
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EasySep™人总粒细胞分选试剂盒免疫磁珠负选试剂盒
标记抗体
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抗人CD45抗体，克隆HI30小鼠单克隆IgG1抗体，抗人、黑猩猩CD45
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抗人CD16抗体，克隆号3G8小鼠抗人、恒河猴、食蟹猴CD16单克隆IgG1抗体
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抗人CD66b抗体，克隆G10F5小鼠单克隆IgM抗体，抗人、黑猩猩CD66b
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抗人CD123（IL-3Rα）抗体，克隆 6H6抗人、恒河猴、白眉猴CD123 （IL-3Rα）的小鼠单克隆IgG1抗体
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
