产品号 #60086_C
小鼠单克隆IgM抗体，抗人、黑猩猩CD66b
总览
G10F5抗体识别CD66b（旧称CD67），CD66b是一种~95 kDa的单链糖基磷脂酰肌醇（GPI）连接的糖蛋白，由中性粒细胞和嗜酸性粒细胞表达。CD66b是免疫球蛋白超家族中癌胚抗原（CEA）样蛋白亚家族的成员，具有两个类Ig的C2型结构域和一个类Ig的V型结构域。在中性粒细胞中，CD66b定位于细胞质中的颗粒内，并在质膜上也表达。粒细胞活化时，CD66b 的表面表达水平会升高，这种效应是由刺激物，如肉豆蔻酸酯（PMA）、钙离子载体和N -甲酰基蛋氨酸-亮氨酸-苯丙氨酸（FMLP）诱导的。CD66b似乎作为一种信号受体，通过调节粒细胞活化和多种蛋白（包括CD66c、半乳糖凝集素-3、纤连蛋白和E -选择素）的粘附，参与细胞粘附、吞噬和趋化作用。
亚型
一抗
靶抗原
CD66b
别名
癌胚抗原相关细胞粘附分子 8，CD67，CEACAM8，CGM6，NCA-95
活性物种
Chimpanzee，人
偶联
APC，FITC，Pacific Blue，PE，未偶联的
宿主物种
小鼠
细胞类型
粒细胞及其亚群，髓系细胞
种属
人，非人灵长类
应用
流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
研究领域
免疫
克隆
G10F5
基因编号
1088
同种型
IgM，kappa
Data Figures
Figure 1. Data for Unconjugated
Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD66b Antibody, Clone G10F5, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30 (Catalog #60069), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).
Figure 2. Data for APC-Conjugated
(A) Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD66b Antibody, Clone G10F5, APC (filled histogram) or a mouse IgM, kappa APC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD15 Positive Selection Kit and labeled with Anti-Human CD66b Antibody, Clone G10F5, APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgM, kappa APC isotype control antibody is shown (solid line histogram). (C) Flow cytometry analysis of whole blood nucleated cells processed with the EasySep™ Human Myeloid Positive Selection Kit and labeled with Anti-Human CD66b Antibody, Clone G10F5, APC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgM, kappa APC isotype control antibody is shown (solid line histogram).
Figure 3. Data for FITC-Conjugated
(A) Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD66b Antibody, Clone G10F5, FITC (filled histogram) or a mouse IgM, kappa FITC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ Whole Blood CD66b Positive Selection Kit and labeled with Anti-Human CD66b Antibody, Clone G10F5, FITC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgM, kappa FITC isotype control antibody is shown (solid line histogram). (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD15 Positive Selection Kit and labeled with Anti-Human CD66b Antibody, Clone G10F5, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgM, kappa FITC isotype control antibody is shown (solid line histogram).
Figure 4. Data for PE-Conjugated
(A) Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD66b Antibody, Clone G10F5, PE (filled histogram) or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE (Catalog #60069PE) (solid line histogram). (B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD15 Positive Selection Kit and labeled with Anti-Human CD66b Antibody, Clone G10F5, PE. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgM, kappa Isotype Control Antibody, Clone MM30, PE is shown (solid line histogram). (C) Flow cytometry analysis of whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with AntiHuman CD66b Antibody, Clone G10F5, PE. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE is shown (solid line histogram).
Figure 5. Data for PB-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD66b Antibody, Clone G10F5, Pacific Blue™ (filled histogram) or a mouse IgM, kappa Pacific Blue™ isotype control antibody (solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Human CD66b Antibody, Clone G10F5, APC Catalog #
60086AZ, 60086AZ.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD66b Antibody, Clone G10F5, FITC Catalog #
60086FI, 60086FI.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD66b Antibody, Clone G10F5, PE Catalog #
60086PE, 60086PE.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD66b Antibody, Clone G10F5 Catalog #
60086 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD66b Antibody, Clone G10F5, APC Catalog #
60086AZ, 60086AZ.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD66b Antibody, Clone G10F5, FITC Catalog #
60086FI, 60086FI.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD66b Antibody, Clone G10F5, PE Catalog #
60086PE, 60086PE.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD66b Antibody, Clone G10F5 Catalog #
60086 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Granulocyte Research
Workflow Stages for Myeloid Cell Research
Workflow Stages for Chimerism Analysis
Resources and Publications
Educational Materials (3)
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更多信息
| Species | Human, Non-Human Primate |
|---|---|
| Clone | G10F5 |
| Gene Id | 1088 |
| Alternative Names | Carcinoembryonic antigen-related cell adhesion molecule 8, CD67, CEACAM8, CGM6, NCA-95 |
| Isotype | IgM, kappa |
Legal Statement: Pacific Blue is a trademark of Life Technologies Corporation. Antibodies conjugated to Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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