EasySep™ HLA T Cell Enrichment Kit

Immunomagnetic positive selection cell isolation kit

产品优势


  • Fast and easy-to-use

  • Up to 99.9% purity

  • No columns required

  • The EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit is designed to isolate CD8+ cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD8 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD8 antigen is expressed on cytotoxic T cells and weakly on a subset of NK cells.

    This product replaces the EasySep™ Human Whole Blood CD8 Positive Selection Kit (Catalog #18083) for even faster cell isolations.

    Data Figures

    Starting with human whole blood, the total CD8+ cell content of the isolated fraction is typically 98.7 ± 1.1% (gated on CD45; mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 2.4% and 95.8%, respectively.

    Protocols and Documentation

    Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

    Document Type
    Product Name
    Catalog #
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    Catalog #
    17889
    Lot #
    1000130607 or higher
    Language
    English
    Catalog #
    17889
    Lot #
    1000130607 or higher
    Language
    English
    Catalog #
    17889RF
    Lot #
    1000130607 or higher
    Language
    English
    Catalog #
    17889RF
    Lot #
    1000130607 or higher
    Language
    English
    Document Type
    Safety Data Sheet 1
    Catalog #
    17889
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 2
    Catalog #
    17889
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 3
    Catalog #
    17889
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 1
    Catalog #
    17889RF
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 2
    Catalog #
    17889RF
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 3
    Catalog #
    17889RF
    Lot #
    All
    Language
    English

    Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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